Nucleic Acid Analysis - Stiffness Analysis
(Using Curves+ program)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||||||||||||
| 5' | C | - | G | - | C | - | G | - | T | - | X | - | G | - | A | - | C | - | G | - | C | - | G | 3' |
| | | x | | | x | | | x | | | x | | | x | | | x | | | x | | | x | | | x | | | ||||||
| 3' | G | - | C | - | G | - | C | - | A | - | G | - | X | - | T | - | G | - | C | - | G | - | C | 5' |
| 24 | 23 | 22 | 21 | 20 | 19 | 18 | 17 | 16 | 15 | 14 | 13 |
Interactive Sequence (see Help)
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 1.075 | 0.000 | 0.000 | -0.052 | 0.000 | 0.000 |
| Slide | 0.000 | 3.869 | 3.152 | 0.000 | 0.013 | -0.063 |
| Rise | 0.000 | 3.152 | 10.567 | 0.000 | 0.070 | -0.232 |
| Tilt | -0.052 | 0.000 | 0.000 | 0.040 | 0.000 | 0.000 |
| Roll | 0.000 | 0.013 | 0.070 | 0.000 | 0.027 | 0.010 |
| Twist | 0.000 | -0.063 | -0.232 | 0.000 | 0.010 | 0.042 |
 |
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 1.192 | 0.000 | 0.000 | -0.153 | 0.000 | 0.000 |
| Slide | 0.000 | 2.602 | 1.351 | 0.000 | 0.024 | -0.084 |
| Rise | 0.000 | 1.351 | 7.750 | 0.000 | -0.059 | -0.217 |
| Tilt | -0.153 | 0.000 | 0.000 | 0.034 | 0.000 | 0.000 |
| Roll | 0.000 | 0.024 | -0.059 | 0.000 | 0.014 | 0.003 |
| Twist | 0.000 | -0.084 | -0.217 | 0.000 | 0.003 | 0.025 |
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[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 1.182 | 0.000 | 0.000 | -0.146 | 0.000 | 0.000 |
| Slide | 0.000 | 2.601 | 1.515 | 0.000 | 0.019 | -0.099 |
| Rise | 0.000 | 1.515 | 7.587 | 0.000 | -0.053 | -0.202 |
| Tilt | -0.146 | 0.000 | 0.000 | 0.034 | 0.000 | 0.000 |
| Roll | 0.000 | 0.019 | -0.053 | 0.000 | 0.014 | 0.002 |
| Twist | 0.000 | -0.099 | -0.202 | 0.000 | 0.002 | 0.023 |
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[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Slide | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Rise | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Tilt | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Roll | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Twist | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
|
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 1.284 | 0.000 | 0.000 | -0.136 | 0.000 | 0.000 |
| Slide | 0.000 | 2.781 | 2.067 | 0.000 | 0.061 | -0.041 |
| Rise | 0.000 | 2.067 | 10.420 | 0.000 | 0.115 | -0.187 |
| Tilt | -0.136 | 0.000 | 0.000 | 0.042 | 0.000 | 0.000 |
| Roll | 0.000 | 0.061 | 0.115 | 0.000 | 0.026 | 0.010 |
| Twist | 0.000 | -0.041 | -0.187 | 0.000 | 0.010 | 0.030 |
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[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 1.331 | 0.000 | 0.000 | -0.146 | 0.000 | 0.000 |
| Slide | 0.000 | 2.757 | 2.392 | 0.000 | 0.071 | -0.044 |
| Rise | 0.000 | 2.392 | 11.195 | 0.000 | 0.141 | -0.214 |
| Tilt | -0.146 | 0.000 | 0.000 | 0.043 | 0.000 | 0.000 |
| Roll | 0.000 | 0.071 | 0.141 | 0.000 | 0.027 | 0.008 |
| Twist | 0.000 | -0.044 | -0.214 | 0.000 | 0.008 | 0.031 |
|
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 0.905 | 0.000 | 0.000 | -0.054 | 0.000 | 0.000 |
| Slide | 0.000 | 3.758 | 3.194 | 0.000 | 0.004 | -0.052 |
| Rise | 0.000 | 3.194 | 10.470 | 0.000 | 0.049 | -0.229 |
| Tilt | -0.054 | 0.000 | 0.000 | 0.040 | 0.000 | 0.000 |
| Roll | 0.000 | 0.004 | 0.049 | 0.000 | 0.028 | 0.012 |
| Twist | 0.000 | -0.052 | -0.229 | 0.000 | 0.012 | 0.043 |
|
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
Download Raw Data |
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Slide | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Rise | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Tilt | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Roll | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Twist | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
|
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
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| Shift | Slide | Rise | Tilt | Roll | Twist | |
| Shift | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Slide | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Rise | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Tilt | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Roll | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| Twist | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
|
[Image courtesy of Curves+] |
Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Ų), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)
|
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| Mean | Stdev | |
| -acgt.8.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -cgcg.3.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -cgcg.9.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -gatx.7.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -gcgc.10.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -gcgc.2.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -gtac.4.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -txga.5.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| -xgxg.6.cte | 0.000 | 0.000 |
| Mean | Stdev | |
| rise-10-GCGC | 3.374 | 0.284 |
| Mean | Stdev | |
| roll-10-GCGC | -1.211 | 5.552 |
| Mean | Stdev | |
| shift-10-GCGC | 0.000 | 0.834 |
| Mean | Stdev | |
| slide-10-GCGC | -0.259 | 0.506 |
| Mean | Stdev | |
| tilt-10-GCGC | 0.000 | 4.617 |
| Mean | Stdev | |
| twist-10-GCGC | 32.897 | 5.349 |
| Mean | Stdev | |
| rise-2-GCGC | 3.381 | 0.282 |
| Mean | Stdev | |
| roll-2-GCGC | -1.681 | 5.346 |
| Mean | Stdev | |
| shift-2-GCGC | 0.000 | 0.837 |
| Mean | Stdev | |
| slide-2-GCGC | -0.307 | 0.520 |
| Mean | Stdev | |
| tilt-2-GCGC | 0.000 | 4.641 |
| Mean | Stdev | |
| twist-2-GCGC | 33.435 | 5.041 |
| Mean | Stdev | |
| rise-3-CGCG | 3.383 | 0.326 |
| Mean | Stdev | |
| roll-3-CGCG | 5.592 | 6.672 |
| Mean | Stdev | |
| shift-3-CGCG | 0.000 | 1.077 |
| Mean | Stdev | |
| slide-3-CGCG | 0.093 | 0.522 |
| Mean | Stdev | |
| tilt-3-CGCG | 0.000 | 6.352 |
| Mean | Stdev | |
| twist-3-CGCG | 38.078 | 5.806 |
| Mean | Stdev | |
| rise-4-GTAC | 3.371 | 0.299 |
| Mean | Stdev | |
| roll-4-GTAC | -0.855 | 5.037 |
| Mean | Stdev | |
| shift-4-GTAC | 0.000 | 0.839 |
| Mean | Stdev | |
| slide-4-GTAC | -0.671 | 0.461 |
| Mean | Stdev | |
| tilt-4-GTAC | 0.000 | 3.980 |
| Mean | Stdev | |
| twist-4-GTAC | 33.003 | 4.276 |
| Mean | Stdev | |
| rise-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| roll-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| shift-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| slide-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| tilt-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| twist-5-TXGA | 0.000 | 0.000 |
| Mean | Stdev | |
| rise-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| roll-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| shift-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| slide-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| tilt-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| twist-6-XGXG | 0.000 | 0.000 |
| Mean | Stdev | |
| rise-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| roll-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| shift-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| slide-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| tilt-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| twist-7-GATX | 0.000 | 0.000 |
| Mean | Stdev | |
| rise-8-ACGT | 3.408 | 0.295 |
| Mean | Stdev | |
| roll-8-ACGT | -1.343 | 5.072 |
| Mean | Stdev | |
| shift-8-ACGT | 0.000 | 0.763 |
| Mean | Stdev | |
| slide-8-ACGT | -0.726 | 0.448 |
| Mean | Stdev | |
| tilt-8-ACGT | 0.000 | 3.943 |
| Mean | Stdev | |
| twist-8-ACGT | 33.506 | 4.338 |
| Mean | Stdev | |
| rise-9-CGCG | 3.398 | 0.328 |
| Mean | Stdev | |
| roll-9-CGCG | 5.324 | 6.587 |
| Mean | Stdev | |
| shift-9-CGCG | 0.000 | 1.035 |
| Mean | Stdev | |
| slide-9-CGCG | 0.074 | 0.537 |
| Mean | Stdev | |
| tilt-9-CGCG | 0.000 | 6.134 |
| Mean | Stdev | |
| twist-9-CGCG | 38.122 | 6.084 |
