NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.787	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.496	1.074	0.000	-0.056	-0.151
Rise	0.000	1.074	8.970	0.000	-0.028	-0.145
Tilt	-0.095	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.056	-0.028	0.000	0.022	0.008
Twist	0.000	-0.151	-0.145	0.000	0.008	0.042

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.678	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.811	0.883	0.000	-0.082	-0.167
Rise	0.000	0.883	9.905	0.000	-0.035	-0.178
Tilt	-0.084	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.082	-0.035	0.000	0.023	0.010
Twist	0.000	-0.167	-0.178	0.000	0.010	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.560	0.000	0.000	-0.065	0.000	0.000
Slide	0.000	4.362	3.606	0.000	0.020	-0.124
Rise	0.000	3.606	11.490	0.000	0.106	-0.197
Tilt	-0.065	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.020	0.106	0.000	0.030	0.008
Twist	0.000	-0.124	-0.197	0.000	0.008	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.142	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	1.714	1.762	0.000	0.002	-0.116
Rise	0.000	1.762	7.610	0.000	-0.007	-0.163
Tilt	-0.103	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.002	-0.007	0.000	0.019	0.003
Twist	0.000	-0.116	-0.163	0.000	0.003	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.797	0.000	0.000	-0.118	0.000	0.000
Slide	0.000	2.161	2.071	0.000	-0.005	-0.150
Rise	0.000	2.071	7.906	0.000	-0.051	-0.174
Tilt	-0.118	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.005	-0.051	0.000	0.023	0.005
Twist	0.000	-0.150	-0.174	0.000	0.005	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.288	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	1.728	1.792	0.000	-0.001	-0.106
Rise	0.000	1.792	8.171	0.000	-0.021	-0.177
Tilt	-0.106	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.001	-0.021	0.000	0.022	0.004
Twist	0.000	-0.106	-0.177	0.000	0.004	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.753	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	2.334	1.499	0.000	-0.050	-0.113
Rise	0.000	1.499	9.752	0.000	-0.033	-0.185
Tilt	-0.045	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.050	-0.033	0.000	0.021	0.006
Twist	0.000	-0.113	-0.185	0.000	0.006	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.712	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	2.445	1.649	0.000	-0.050	-0.118
Rise	0.000	1.649	9.828	0.000	-0.038	-0.200
Tilt	-0.051	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.050	-0.038	0.000	0.019	0.005
Twist	0.000	-0.118	-0.200	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.417	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	2.924	0.713	0.000	0.015	-0.164
Rise	0.000	0.713	8.710	0.000	-0.132	-0.254
Tilt	-0.054	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.015	-0.132	0.000	0.020	0.014
Twist	0.000	-0.164	-0.254	0.000	0.014	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.404	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.642	0.558	0.000	0.012	-0.149
Rise	0.000	0.558	9.075	0.000	-0.117	-0.221
Tilt	-0.048	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.012	-0.117	0.000	0.019	0.013
Twist	0.000	-0.149	-0.221	0.000	0.013	0.028

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-acgt.6.cte	0.000	0.000

	Mean	Stdev
-ccgg.2.cte	0.000	0.000

	Mean	Stdev
-ccgg.7.cte	0.000	0.000

	Mean	Stdev
-ccgg.8.cte	0.000	0.000

	Mean	Stdev
-ctag.3.cte	0.000	0.000

	Mean	Stdev
-ctag.9.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.098	0.301

	Mean	Stdev
roll-10-TATA	4.974	7.670

	Mean	Stdev
shift-10-TATA	0.000	1.395

	Mean	Stdev
slide-10-TATA	0.258	0.629

	Mean	Stdev
tilt-10-TATA	0.000	5.737

	Mean	Stdev
twist-10-TATA	33.630	7.761

	Mean	Stdev
rise-11-AATT	3.250	0.266

	Mean	Stdev
roll-11-AATT	0.112	5.403

	Mean	Stdev
shift-11-AATT	0.000	1.083

	Mean	Stdev
slide-11-AATT	0.037	0.558

	Mean	Stdev
tilt-11-AATT	0.000	5.404

	Mean	Stdev
twist-11-AATT	36.332	4.344

	Mean	Stdev
rise-2-CCGG	3.521	0.320

	Mean	Stdev
roll-2-CCGG	3.246	5.616

	Mean	Stdev
shift-2-CCGG	0.000	0.832

	Mean	Stdev
slide-2-CCGG	-0.525	0.739

	Mean	Stdev
tilt-2-CCGG	0.000	4.694

	Mean	Stdev
twist-2-CCGG	35.171	5.170

	Mean	Stdev
rise-3-CTAG	3.138	0.270

	Mean	Stdev
roll-3-CTAG	1.413	5.571

	Mean	Stdev
shift-3-CTAG	0.000	0.924

	Mean	Stdev
slide-3-CTAG	-0.288	0.585

	Mean	Stdev
tilt-3-CTAG	0.000	4.659

	Mean	Stdev
twist-3-CTAG	31.246	6.077

	Mean	Stdev
rise-4-TATA	3.064	0.286

	Mean	Stdev
roll-4-TATA	4.652	7.496

	Mean	Stdev
shift-4-TATA	0.000	1.385

	Mean	Stdev
slide-4-TATA	0.295	0.655

	Mean	Stdev
tilt-4-TATA	0.000	5.700

	Mean	Stdev
twist-4-TATA	33.779	7.962

	Mean	Stdev
rise-5-AATT	3.193	0.255

	Mean	Stdev
roll-5-AATT	1.150	5.471

	Mean	Stdev
shift-5-AATT	0.000	1.123

	Mean	Stdev
slide-5-AATT	0.062	0.540

	Mean	Stdev
tilt-5-AATT	0.000	5.073

	Mean	Stdev
twist-5-AATT	35.537	4.867

	Mean	Stdev
rise-6-ACGT	3.386	0.273

	Mean	Stdev
roll-6-ACGT	-0.848	4.656

	Mean	Stdev
shift-6-ACGT	0.000	0.633

	Mean	Stdev
slide-6-ACGT	-0.805	0.433

	Mean	Stdev
tilt-6-ACGT	0.000	3.836

	Mean	Stdev
twist-6-ACGT	33.546	4.220

	Mean	Stdev
rise-7-CCGG	3.647	0.318

	Mean	Stdev
roll-7-CCGG	4.843	5.174

	Mean	Stdev
shift-7-CCGG	0.000	0.638

	Mean	Stdev
slide-7-CCGG	-1.004	0.676

	Mean	Stdev
tilt-7-CCGG	0.000	4.326

	Mean	Stdev
twist-7-CCGG	35.906	4.671

	Mean	Stdev
rise-8-CCGG	3.488	0.310

	Mean	Stdev
roll-8-CCGG	3.539	5.232

	Mean	Stdev
shift-8-CCGG	0.000	0.768

	Mean	Stdev
slide-8-CCGG	-0.670	0.714

	Mean	Stdev
tilt-8-CCGG	0.000	4.469

	Mean	Stdev
twist-8-CCGG	34.711	5.224

	Mean	Stdev
rise-9-CTAG	3.152	0.272

	Mean	Stdev
roll-9-CTAG	1.804	5.796

	Mean	Stdev
shift-9-CTAG	0.000	0.968

	Mean	Stdev
slide-9-CTAG	-0.284	0.574

	Mean	Stdev
tilt-9-CTAG	0.000	4.727

	Mean	Stdev
twist-9-CTAG	31.957	5.914

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