NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.537	0.000	0.000	-0.022	0.000	0.000
Slide	0.000	2.548	1.518	0.000	-0.098	-0.118
Rise	0.000	1.518	6.307	0.000	-0.071	-0.155
Tilt	-0.022	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.098	-0.071	0.000	0.024	0.011
Twist	0.000	-0.118	-0.155	0.000	0.011	0.030

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.266	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	2.252	1.357	0.000	-0.045	-0.128
Rise	0.000	1.357	7.114	0.000	-0.051	-0.173
Tilt	-0.062	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.045	-0.051	0.000	0.019	0.005
Twist	0.000	-0.128	-0.173	0.000	0.005	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.160	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	2.334	1.354	0.000	-0.088	-0.136
Rise	0.000	1.354	6.675	0.000	-0.052	-0.152
Tilt	-0.028	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.088	-0.052	0.000	0.022	0.008
Twist	0.000	-0.136	-0.152	0.000	0.008	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.214	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	2.533	1.305	0.000	-0.072	-0.160
Rise	0.000	1.305	6.858	0.000	-0.056	-0.177
Tilt	-0.055	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.072	-0.056	0.000	0.021	0.008
Twist	0.000	-0.160	-0.176	0.000	0.008	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.431	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.872	1.280	0.000	-0.058	-0.189
Rise	0.000	1.280	8.093	0.000	-0.041	-0.181
Tilt	-0.059	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.058	-0.041	0.000	0.023	0.008
Twist	0.000	-0.189	-0.181	0.000	0.008	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.245	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	3.270	2.727	0.000	0.014	-0.116
Rise	0.000	2.727	10.567	0.000	0.066	-0.199
Tilt	-0.053	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.014	0.066	0.000	0.025	0.010
Twist	0.000	-0.116	-0.199	0.000	0.010	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.013	0.000	0.000	-0.111	0.000	0.000
Slide	0.000	2.563	0.540	0.000	0.007	-0.123
Rise	0.000	0.540	7.605	0.000	-0.073	-0.221
Tilt	-0.111	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.007	-0.073	0.000	0.016	0.007
Twist	0.000	-0.123	-0.221	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.781	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.186	1.238	0.000	-0.055	-0.131
Rise	0.000	1.238	7.252	0.000	-0.059	-0.154
Tilt	-0.088	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.055	-0.059	0.000	0.022	0.011
Twist	0.000	-0.131	-0.154	0.000	0.011	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.249	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	3.157	2.111	0.000	0.052	-0.056
Rise	0.000	2.111	10.184	0.000	0.123	-0.137
Tilt	-0.105	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.052	0.123	0.000	0.024	0.005
Twist	0.000	-0.056	-0.137	0.000	0.005	0.019

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-acgt.10.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
rise-10-ACGT	3.402	0.276

	Mean	Stdev
roll-10-ACGT	-1.561	5.229

	Mean	Stdev
shift-10-ACGT	0.000	0.707

	Mean	Stdev
slide-10-ACGT	-0.765	0.490

	Mean	Stdev
tilt-10-ACGT	0.000	3.927

	Mean	Stdev
twist-10-ACGT	33.061	4.307

	Mean	Stdev
rise-2-GCGC	3.399	0.279

	Mean	Stdev
roll-2-GCGC	-1.539	5.444

	Mean	Stdev
shift-2-GCGC	0.000	0.779

	Mean	Stdev
slide-2-GCGC	-0.314	0.474

	Mean	Stdev
tilt-2-GCGC	0.000	4.408

	Mean	Stdev
twist-2-GCGC	31.931	6.326

	Mean	Stdev
rise-3-CGCG	3.116	0.335

	Mean	Stdev
roll-3-CGCG	5.645	6.724

	Mean	Stdev
shift-3-CGCG	0.000	1.082

	Mean	Stdev
slide-3-CGCG	0.123	0.589

	Mean	Stdev
tilt-3-CGCG	0.000	7.042

	Mean	Stdev
twist-3-CGCG	32.600	7.642

	Mean	Stdev
rise-4-GATC	3.384	0.305

	Mean	Stdev
roll-4-GATC	3.691	5.679

	Mean	Stdev
shift-4-GATC	0.000	1.040

	Mean	Stdev
slide-4-GATC	0.167	0.605

	Mean	Stdev
tilt-4-GATC	0.000	5.121

	Mean	Stdev
twist-4-GATC	36.113	5.209

	Mean	Stdev
rise-5-AATT	3.352	0.339

	Mean	Stdev
roll-5-AATT	2.551	5.649

	Mean	Stdev
shift-5-AATT	0.000	0.621

	Mean	Stdev
slide-5-AATT	-0.555	0.573

	Mean	Stdev
tilt-5-AATT	0.000	4.287

	Mean	Stdev
twist-5-AATT	33.095	5.275

	Mean	Stdev
rise-6-AATT	3.359	0.318

	Mean	Stdev
roll-6-AATT	1.727	5.760

	Mean	Stdev
shift-6-AATT	0.000	0.718

	Mean	Stdev
slide-6-AATT	-0.436	0.614

	Mean	Stdev
tilt-6-AATT	0.000	4.688

	Mean	Stdev
twist-6-AATT	34.422	5.941

	Mean	Stdev
rise-7-AATT	3.334	0.323

	Mean	Stdev
roll-7-AATT	2.401	5.610

	Mean	Stdev
shift-7-AATT	0.000	0.718

	Mean	Stdev
slide-7-AATT	-0.545	0.631

	Mean	Stdev
tilt-7-AATT	0.000	4.382

	Mean	Stdev
twist-7-AATT	33.085	5.734

	Mean	Stdev
rise-8-AATT	3.384	0.319

	Mean	Stdev
roll-8-AATT	1.739	5.696

	Mean	Stdev
shift-8-AATT	0.000	0.722

	Mean	Stdev
slide-8-AATT	-0.503	0.603

	Mean	Stdev
tilt-8-AATT	0.000	4.357

	Mean	Stdev
twist-8-AATT	35.058	5.616

	Mean	Stdev
rise-9-AATT	3.297	0.287

	Mean	Stdev
roll-9-AATT	0.397	5.315

	Mean	Stdev
shift-9-AATT	0.000	0.671

	Mean	Stdev
slide-9-AATT	-0.548	0.562

	Mean	Stdev
tilt-9-AATT	0.000	4.855

	Mean	Stdev
twist-9-AATT	35.215	5.192

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