NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.394	0.000	0.000	0.004	0.000	0.000
Slide	0.000	2.420	0.478	0.000	-0.118	-0.145
Rise	0.000	0.478	7.287	0.000	-0.124	-0.098
Tilt	0.004	0.000	0.000	0.016	0.000	0.000
Roll	0.000	-0.118	-0.124	0.000	0.018	0.014
Twist	0.000	-0.145	-0.098	0.000	0.014	0.028

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.288	0.000	0.000	-0.148	0.000	0.000
Slide	0.000	3.436	1.720	0.000	-0.049	-0.181
Rise	0.000	1.720	6.726	0.000	-0.070	-0.231
Tilt	-0.148	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.049	-0.070	0.000	0.021	0.005
Twist	0.000	-0.181	-0.231	0.000	0.005	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.423	0.000	0.000	0.004	0.000	0.000
Slide	0.000	4.245	1.986	0.000	-0.060	-0.225
Rise	0.000	1.986	5.006	0.000	-0.109	-0.110
Tilt	0.004	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.060	-0.109	0.000	0.032	0.006
Twist	0.000	-0.225	-0.110	0.000	0.006	0.067

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.713	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	5.646	2.640	0.000	-0.039	-0.103
Rise	0.000	2.640	7.621	0.000	-0.130	-0.171
Tilt	-0.028	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.039	-0.130	0.000	0.017	0.007
Twist	0.000	-0.103	-0.171	0.000	0.007	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.830	0.000	0.000	0.000	0.000	0.000
Slide	0.000	4.408	2.867	0.000	-0.037	-0.254
Rise	0.000	2.867	7.692	0.000	0.025	-0.124
Tilt	0.000	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.037	0.025	0.000	0.032	-0.002
Twist	0.000	-0.254	-0.124	0.000	-0.002	0.068

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.451	0.000	0.000	-0.019	0.000	0.000
Slide	0.000	3.415	-0.348	0.000	0.025	-0.096
Rise	0.000	-0.348	7.830	0.000	-0.192	-0.248
Tilt	-0.019	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.025	-0.192	0.000	0.019	0.012
Twist	0.000	-0.096	-0.248	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.877	0.000	0.000	-0.096	0.000	0.000
Slide	0.000	2.704	1.231	0.000	-0.024	-0.074
Rise	0.000	1.231	7.705	0.000	-0.168	-0.237
Tilt	-0.096	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.024	-0.168	0.000	0.024	0.011
Twist	0.000	-0.074	-0.237	0.000	0.011	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.233	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	5.964	3.774	0.000	-0.008	-0.188
Rise	0.000	3.774	11.693	0.000	0.083	-0.188
Tilt	-0.033	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.008	0.083	0.000	0.026	-0.000
Twist	0.000	-0.188	-0.188	0.000	-0.000	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.837	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	4.429	3.517	0.000	0.102	-0.092
Rise	0.000	3.517	11.988	0.000	0.181	-0.212
Tilt	-0.009	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.102	0.181	0.000	0.026	0.001
Twist	0.000	-0.092	-0.212	0.000	0.001	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.366	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	5.849	2.514	0.000	-0.070	-0.255
Rise	0.000	2.514	10.135	0.000	-0.004	-0.078
Tilt	-0.074	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.070	-0.004	0.000	0.029	0.009
Twist	0.000	-0.255	-0.078	0.000	0.009	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.501	0.000	0.000	-0.003	0.000	0.000
Slide	0.000	5.005	-0.359	0.000	0.018	-0.139
Rise	0.000	-0.359	6.162	0.000	-0.217	-0.128
Tilt	-0.003	0.000	0.000	0.010	0.000	0.000
Roll	0.000	0.018	-0.217	0.000	0.027	0.011
Twist	0.000	-0.139	-0.128	0.000	0.011	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.201	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	2.670	-0.547	0.000	-0.042	-0.103
Rise	0.000	-0.547	5.604	0.000	-0.231	-0.040
Tilt	-0.011	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.042	-0.231	0.000	0.020	0.002
Twist	0.000	-0.103	-0.040	0.000	0.002	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.928	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	4.673	1.400	0.000	0.006	-0.220
Rise	0.000	1.400	7.081	0.000	0.045	-0.076
Tilt	-0.120	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.006	0.045	0.000	0.018	-0.005
Twist	0.000	-0.220	-0.076	0.000	-0.005	0.076

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.575	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	3.485	-0.045	0.000	0.021	0.055
Rise	0.000	-0.045	6.258	0.000	-0.161	-0.041
Tilt	-0.079	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.021	-0.161	0.000	0.013	0.005
Twist	0.000	0.055	-0.041	0.000	0.005	0.059

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.4.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-acgt.5.cte	0.000	0.000

	Mean	Stdev
-atat.9.cte	0.000	0.000

	Mean	Stdev
-catg.3.cte	0.000	0.000

	Mean	Stdev
-catg.6.cte	0.000	0.000

	Mean	Stdev
-gcgc.15.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gtac.12.cte	0.000	0.000

	Mean	Stdev
-tgca.11.cte	0.000	0.000

	Mean	Stdev
-tgca.14.cte	0.000	0.000

	Mean	Stdev
-ttaa.10.cte	0.000	0.000

	Mean	Stdev
-ttaa.13.cte	0.000	0.000

	Mean	Stdev
rise-10-TTAA	3.482	0.299

	Mean	Stdev
roll-10-TTAA	-4.590	5.748

	Mean	Stdev
shift-10-TTAA	0.000	1.071

	Mean	Stdev
slide-10-TTAA	-0.158	0.392

	Mean	Stdev
tilt-10-TTAA	0.000	5.567

	Mean	Stdev
twist-10-TTAA	36.348	3.007

	Mean	Stdev
rise-11-TGCA	3.349	0.377

	Mean	Stdev
roll-11-TGCA	24.777	6.329

	Mean	Stdev
shift-11-TGCA	0.000	1.082

	Mean	Stdev
slide-11-TGCA	-0.314	0.435

	Mean	Stdev
tilt-11-TGCA	0.000	7.495

	Mean	Stdev
twist-11-TGCA	15.593	9.009

	Mean	Stdev
rise-12-GTAC	3.202	0.255

	Mean	Stdev
roll-12-GTAC	2.087	4.688

	Mean	Stdev
shift-12-GTAC	0.000	1.599

	Mean	Stdev
slide-12-GTAC	-0.169	0.402

	Mean	Stdev
tilt-12-GTAC	0.000	4.823

	Mean	Stdev
twist-12-GTAC	31.315	5.073

	Mean	Stdev
rise-13-TTAA	3.439	0.370

	Mean	Stdev
roll-13-TTAA	10.864	8.170

	Mean	Stdev
shift-13-TTAA	0.000	1.348

	Mean	Stdev
slide-13-TTAA	0.897	0.415

	Mean	Stdev
tilt-13-TTAA	0.000	6.494

	Mean	Stdev
twist-13-TTAA	32.767	3.215

	Mean	Stdev
rise-14-TGCA	3.957	0.505

	Mean	Stdev
roll-14-TGCA	34.545	8.436

	Mean	Stdev
shift-14-TGCA	0.000	1.725

	Mean	Stdev
slide-14-TGCA	0.700	0.590

	Mean	Stdev
tilt-14-TGCA	0.000	4.431

	Mean	Stdev
twist-14-TGCA	25.234	6.131

	Mean	Stdev
rise-15-GCGC	3.248	0.258

	Mean	Stdev
roll-15-GCGC	-0.909	4.824

	Mean	Stdev
shift-15-GCGC	0.000	0.517

	Mean	Stdev
slide-15-GCGC	-0.290	0.379

	Mean	Stdev
tilt-15-GCGC	0.000	4.519

	Mean	Stdev
twist-15-GCGC	24.477	5.157

	Mean	Stdev
rise-2-GCGC	3.270	0.269

	Mean	Stdev
roll-2-GCGC	-2.204	5.122

	Mean	Stdev
shift-2-GCGC	0.000	0.838

	Mean	Stdev
slide-2-GCGC	-0.084	0.425

	Mean	Stdev
tilt-2-GCGC	0.000	3.986

	Mean	Stdev
twist-2-GCGC	32.541	4.252

	Mean	Stdev
rise-3-CATG	3.125	0.353

	Mean	Stdev
roll-3-CATG	12.174	7.212

	Mean	Stdev
shift-3-CATG	0.000	1.159

	Mean	Stdev
slide-3-CATG	0.083	0.482

	Mean	Stdev
tilt-3-CATG	0.000	5.041

	Mean	Stdev
twist-3-CATG	27.464	7.225

	Mean	Stdev
rise-4-AATT	3.262	0.303

	Mean	Stdev
roll-4-AATT	4.966	8.013

	Mean	Stdev
shift-4-AATT	0.000	1.222

	Mean	Stdev
slide-4-AATT	-0.272	0.628

	Mean	Stdev
tilt-4-AATT	0.000	6.012

	Mean	Stdev
twist-4-AATT	32.814	6.183

	Mean	Stdev
rise-5-ACGT	3.409	0.329

	Mean	Stdev
roll-5-ACGT	7.398	6.423

	Mean	Stdev
shift-5-ACGT	0.000	0.922

	Mean	Stdev
slide-5-ACGT	-0.596	0.355

	Mean	Stdev
tilt-5-ACGT	0.000	4.285

	Mean	Stdev
twist-5-ACGT	27.624	4.386

	Mean	Stdev
rise-6-CATG	3.180	0.334

	Mean	Stdev
roll-6-CATG	11.307	5.588

	Mean	Stdev
shift-6-CATG	0.000	1.083

	Mean	Stdev
slide-6-CATG	0.031	0.491

	Mean	Stdev
tilt-6-CATG	0.000	6.467

	Mean	Stdev
twist-6-CATG	31.878	5.665

	Mean	Stdev
rise-7-AATT	3.562	0.338

	Mean	Stdev
roll-7-AATT	6.224	5.445

	Mean	Stdev
shift-7-AATT	0.000	1.021

	Mean	Stdev
slide-7-AATT	-0.505	0.485

	Mean	Stdev
tilt-7-AATT	0.000	6.674

	Mean	Stdev
twist-7-AATT	39.231	4.778

	Mean	Stdev
rise-8-AATT	3.527	0.390

	Mean	Stdev
roll-8-AATT	2.342	4.505

	Mean	Stdev
shift-8-AATT	0.000	0.492

	Mean	Stdev
slide-8-AATT	-1.375	0.446

	Mean	Stdev
tilt-8-AATT	0.000	4.382

	Mean	Stdev
twist-8-AATT	29.403	3.269

	Mean	Stdev
rise-9-ATAT	3.570	0.320

	Mean	Stdev
roll-9-ATAT	-2.947	4.356

	Mean	Stdev
shift-9-ATAT	0.000	0.840

	Mean	Stdev
slide-9-ATAT	-1.508	0.474

	Mean	Stdev
tilt-9-ATAT	0.000	3.764

	Mean	Stdev
twist-9-ATAT	31.944	3.335

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