NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.669	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	4.184	2.787	0.000	0.005	-0.105
Rise	0.000	2.787	10.495	0.000	0.086	-0.206
Tilt	-0.029	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.005	0.086	0.000	0.021	0.006
Twist	0.000	-0.105	-0.207	0.000	0.006	0.030

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.338	0.000	0.000	0.003	0.000	0.000
Slide	0.000	4.706	3.125	0.000	-0.059	-0.066
Rise	0.000	3.125	10.820	0.000	0.071	-0.135
Tilt	0.003	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.059	0.071	0.000	0.027	0.010
Twist	0.000	-0.066	-0.135	0.000	0.010	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.661	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.346	1.066	0.000	0.013	-0.097
Rise	0.000	1.066	6.959	0.000	-0.063	-0.187
Tilt	-0.077	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.013	-0.063	0.000	0.016	0.008
Twist	0.000	-0.097	-0.187	0.000	0.008	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.100	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	2.123	1.424	0.000	0.012	-0.082
Rise	0.000	1.424	6.229	0.000	-0.032	-0.169
Tilt	-0.120	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.012	-0.032	0.000	0.013	0.003
Twist	0.000	-0.082	-0.169	0.000	0.003	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.817	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	2.229	1.602	0.000	-0.048	-0.125
Rise	0.000	1.602	7.514	0.000	-0.036	-0.170
Tilt	-0.098	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.048	-0.036	0.000	0.018	0.002
Twist	0.000	-0.125	-0.170	0.000	0.002	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.107	0.000	0.000	-0.115	0.000	0.000
Slide	0.000	2.895	2.374	0.000	0.067	-0.069
Rise	0.000	2.374	10.400	0.000	0.130	-0.203
Tilt	-0.115	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.067	0.130	0.000	0.025	0.007
Twist	0.000	-0.069	-0.203	0.000	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.589	0.000	0.000	-0.135	0.000	0.000
Slide	0.000	2.225	2.267	0.000	0.035	-0.050
Rise	0.000	2.267	10.731	0.000	0.121	-0.191
Tilt	-0.135	0.000	0.000	0.035	0.000	0.000
Roll	0.000	0.035	0.121	0.000	0.026	0.007
Twist	0.000	-0.050	-0.191	0.000	0.007	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.441	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	2.423	0.668	0.000	0.015	-0.139
Rise	0.000	0.668	8.176	0.000	-0.110	-0.222
Tilt	-0.051	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.015	-0.110	0.000	0.018	0.013
Twist	0.000	-0.139	-0.222	0.000	0.013	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.749	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	2.153	1.304	0.000	0.006	-0.097
Rise	0.000	1.304	5.988	0.000	-0.057	-0.163
Tilt	-0.074	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.006	-0.057	0.000	0.015	0.006
Twist	0.000	-0.097	-0.163	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.9.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-catg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.10.cte	0.000	0.000

	Mean	Stdev
-ctag.7.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gcgc.6.cte	0.000	0.000

	Mean	Stdev
-tata.8.cte	0.000	0.000

	Mean	Stdev
-tgca.5.cte	0.000	0.000

	Mean	Stdev
rise-10-CGCG	3.399	0.358

	Mean	Stdev
roll-10-CGCG	5.009	6.912

	Mean	Stdev
shift-10-CGCG	0.000	0.985

	Mean	Stdev
slide-10-CGCG	-0.008	0.598

	Mean	Stdev
tilt-10-CGCG	0.000	6.083

	Mean	Stdev
twist-10-CGCG	38.308	6.030

	Mean	Stdev
rise-2-GCGC	3.405	0.288

	Mean	Stdev
roll-2-GCGC	-1.192	5.550

	Mean	Stdev
shift-2-GCGC	0.000	0.865

	Mean	Stdev
slide-2-GCGC	-0.317	0.512

	Mean	Stdev
tilt-2-GCGC	0.000	4.509

	Mean	Stdev
twist-2-GCGC	33.924	5.030

	Mean	Stdev
rise-3-CATG	3.294	0.328

	Mean	Stdev
roll-3-CATG	6.671	6.848

	Mean	Stdev
shift-3-CATG	0.000	1.151

	Mean	Stdev
slide-3-CATG	-0.073	0.573

	Mean	Stdev
tilt-3-CATG	0.000	5.666

	Mean	Stdev
twist-3-CATG	35.305	6.573

	Mean	Stdev
rise-4-ATAT	3.201	0.275

	Mean	Stdev
roll-4-ATAT	-0.477	5.189

	Mean	Stdev
shift-4-ATAT	0.000	0.662

	Mean	Stdev
slide-4-ATAT	-0.717	0.406

	Mean	Stdev
tilt-4-ATAT	0.000	3.742

	Mean	Stdev
twist-4-ATAT	29.250	4.479

	Mean	Stdev
rise-5-TGCA	3.429	0.357

	Mean	Stdev
roll-5-TGCA	7.617	6.850

	Mean	Stdev
shift-5-TGCA	0.000	1.046

	Mean	Stdev
slide-5-TGCA	-0.311	0.605

	Mean	Stdev
tilt-5-TGCA	0.000	5.641

	Mean	Stdev
twist-5-TGCA	35.565	6.025

	Mean	Stdev
rise-6-GCGC	3.481	0.283

	Mean	Stdev
roll-6-GCGC	-0.870	5.125

	Mean	Stdev
shift-6-GCGC	0.000	0.740

	Mean	Stdev
slide-6-GCGC	-0.687	0.581

	Mean	Stdev
tilt-6-GCGC	0.000	4.959

	Mean	Stdev
twist-6-GCGC	35.553	4.480

	Mean	Stdev
rise-7-CTAG	3.322	0.313

	Mean	Stdev
roll-7-CTAG	2.557	5.807

	Mean	Stdev
shift-7-CTAG	0.000	1.083

	Mean	Stdev
slide-7-CTAG	-0.327	0.639

	Mean	Stdev
tilt-7-CTAG	0.000	5.618

	Mean	Stdev
twist-7-CTAG	34.462	6.015

	Mean	Stdev
rise-8-TATA	3.144	0.298

	Mean	Stdev
roll-8-TATA	5.139	7.722

	Mean	Stdev
shift-8-TATA	0.000	1.323

	Mean	Stdev
slide-8-TATA	0.186	0.642

	Mean	Stdev
tilt-8-TATA	0.000	5.675

	Mean	Stdev
twist-8-TATA	34.411	6.942

	Mean	Stdev
rise-9-ACGT	3.259	0.283

	Mean	Stdev
roll-9-ACGT	-1.340	5.704

	Mean	Stdev
shift-9-ACGT	0.000	0.951

	Mean	Stdev
slide-9-ACGT	-0.497	0.419

	Mean	Stdev
tilt-9-ACGT	0.000	3.936

	Mean	Stdev
twist-9-ACGT	29.617	5.132

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