NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.501	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	4.644	2.825	0.000	-0.076	-0.120
Rise	0.000	2.825	10.357	0.000	0.059	-0.171
Tilt	-0.002	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.076	0.059	0.000	0.031	0.014
Twist	0.000	-0.120	-0.171	0.000	0.014	0.045

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.213	0.000	0.000	0.010	0.000	0.000
Slide	0.000	4.642	2.695	0.000	-0.052	-0.078
Rise	0.000	2.695	11.149	0.000	0.087	-0.101
Tilt	0.010	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.052	0.087	0.000	0.026	0.010
Twist	0.000	-0.078	-0.101	0.000	0.010	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.651	0.000	0.000	0.003	0.000	0.000
Slide	0.000	4.517	2.574	0.000	-0.059	-0.089
Rise	0.000	2.574	10.586	0.000	0.069	-0.117
Tilt	0.003	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.059	0.069	0.000	0.030	0.010
Twist	0.000	-0.089	-0.117	0.000	0.010	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.076	0.000	0.000	-0.078	0.000	0.000
Slide	0.000	1.615	0.948	0.000	-0.026	-0.099
Rise	0.000	0.948	6.251	0.000	-0.058	-0.117
Tilt	-0.078	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.026	-0.058	0.000	0.020	0.008
Twist	0.000	-0.099	-0.117	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.477	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	1.978	1.285	0.000	0.017	-0.110
Rise	0.000	1.285	6.600	0.000	-0.068	-0.197
Tilt	-0.055	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.017	-0.068	0.000	0.014	0.008
Twist	0.000	-0.110	-0.197	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.499	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	1.719	1.104	0.000	0.011	-0.104
Rise	0.000	1.104	6.543	0.000	-0.069	-0.188
Tilt	-0.054	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.011	-0.069	0.000	0.014	0.009
Twist	0.000	-0.104	-0.188	0.000	0.009	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.372	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	2.390	1.603	0.000	-0.024	-0.161
Rise	0.000	1.603	7.084	0.000	-0.053	-0.147
Tilt	-0.081	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.024	-0.053	0.000	0.020	0.008
Twist	0.000	-0.161	-0.147	0.000	0.008	0.039

[Image courtesy of Curves+]

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	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-gatc.2.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.6.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
rise-2-GATC	3.485	0.326

	Mean	Stdev
roll-2-GATC	1.281	5.703

	Mean	Stdev
shift-2-GATC	0.000	0.820

	Mean	Stdev
slide-2-GATC	-0.309	0.685

	Mean	Stdev
tilt-2-GATC	0.000	4.942

	Mean	Stdev
twist-2-GATC	37.952	5.002

	Mean	Stdev
rise-3-ATAT	3.257	0.276

	Mean	Stdev
roll-3-ATAT	-1.434	4.935

	Mean	Stdev
shift-3-ATAT	0.000	0.625

	Mean	Stdev
slide-3-ATAT	-0.830	0.405

	Mean	Stdev
tilt-3-ATAT	0.000	3.656

	Mean	Stdev
twist-3-ATAT	30.728	4.122

	Mean	Stdev
rise-4-TATA	3.392	0.341

	Mean	Stdev
roll-4-TATA	5.333	7.502

	Mean	Stdev
shift-4-TATA	0.000	1.280

	Mean	Stdev
slide-4-TATA	-0.062	0.661

	Mean	Stdev
tilt-4-TATA	0.000	5.589

	Mean	Stdev
twist-4-TATA	37.961	5.614

	Mean	Stdev
rise-5-ATAT	3.149	0.260

	Mean	Stdev
roll-5-ATAT	-0.353	5.329

	Mean	Stdev
shift-5-ATAT	0.000	0.696

	Mean	Stdev
slide-5-ATAT	-0.730	0.395

	Mean	Stdev
tilt-5-ATAT	0.000	3.765

	Mean	Stdev
twist-5-ATAT	28.933	4.691

	Mean	Stdev
rise-6-TATA	3.396	0.338

	Mean	Stdev
roll-6-TATA	5.929	7.721

	Mean	Stdev
shift-6-TATA	0.000	1.255

	Mean	Stdev
slide-6-TATA	-0.087	0.706

	Mean	Stdev
tilt-6-TATA	0.000	5.853

	Mean	Stdev
twist-6-TATA	38.042	5.772

	Mean	Stdev
rise-7-ATAT	3.221	0.263

	Mean	Stdev
roll-7-ATAT	-1.455	4.834

	Mean	Stdev
shift-7-ATAT	0.000	0.596

	Mean	Stdev
slide-7-ATAT	-0.817	0.400

	Mean	Stdev
tilt-7-ATAT	0.000	3.694

	Mean	Stdev
twist-7-ATAT	30.020	4.283

	Mean	Stdev
rise-8-TCGA	3.481	0.315

	Mean	Stdev
roll-8-TCGA	0.916	5.678

	Mean	Stdev
shift-8-TCGA	0.000	0.712

	Mean	Stdev
slide-8-TCGA	-0.411	0.611

	Mean	Stdev
tilt-8-TCGA	0.000	4.791

	Mean	Stdev
twist-8-TCGA	38.730	4.709

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