NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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5'	G	-	C	-	G	-	C	-	A	-	T	-	G	-	C	-	T	-	A	-	C	-	G	-	C	-	G	3'
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3'	C	-	G	-	C	-	G	-	T	-	A	-	C	-	G	-	A	-	T	-	G	-	C	-	G	-	C	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.634	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	3.775	2.748	0.000	0.034	-0.107
Rise	0.000	2.748	10.199	0.000	0.100	-0.219
Tilt	-0.028	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.034	0.100	0.000	0.020	0.004
Twist	0.000	-0.107	-0.219	0.000	0.004	0.029

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.349	0.000	0.000	-0.004	0.000	0.000
Slide	0.000	4.750	3.240	0.000	-0.069	-0.090
Rise	0.000	3.240	10.921	0.000	0.044	-0.162
Tilt	-0.004	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.069	0.044	0.000	0.028	0.014
Twist	0.000	-0.090	-0.162	0.000	0.014	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.670	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.336	1.117	0.000	0.016	-0.091
Rise	0.000	1.117	7.049	0.000	-0.070	-0.186
Tilt	-0.077	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.016	-0.070	0.000	0.016	0.008
Twist	0.000	-0.091	-0.186	0.000	0.008	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.045	0.000	0.000	-0.116	0.000	0.000
Slide	0.000	2.083	1.024	0.000	0.011	-0.080
Rise	0.000	1.024	6.620	0.000	-0.047	-0.197
Tilt	-0.116	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.011	-0.047	0.000	0.015	0.005
Twist	0.000	-0.080	-0.197	0.000	0.005	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.069	0.000	0.000	-0.125	0.000	0.000
Slide	0.000	2.402	0.905	0.000	0.021	-0.089
Rise	0.000	0.905	6.813	0.000	-0.036	-0.193
Tilt	-0.125	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.021	-0.036	0.000	0.015	0.004
Twist	0.000	-0.089	-0.193	0.000	0.004	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.818	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	2.359	1.918	0.000	-0.047	-0.144
Rise	0.000	1.918	7.783	0.000	-0.033	-0.182
Tilt	-0.101	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.047	-0.033	0.000	0.019	0.002
Twist	0.000	-0.144	-0.182	0.000	0.002	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.096	0.000	0.000	-0.122	0.000	0.000
Slide	0.000	2.751	2.222	0.000	0.069	-0.056
Rise	0.000	2.222	10.373	0.000	0.131	-0.196
Tilt	-0.122	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.069	0.131	0.000	0.023	0.005
Twist	0.000	-0.056	-0.196	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.158	0.000	0.000	-0.138	0.000	0.000
Slide	0.000	2.894	2.468	0.000	0.065	-0.077
Rise	0.000	2.468	10.469	0.000	0.129	-0.222
Tilt	-0.138	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.065	0.129	0.000	0.025	0.006
Twist	0.000	-0.077	-0.222	0.000	0.006	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.488	0.000	0.000	-0.140	0.000	0.000
Slide	0.000	2.325	2.411	0.000	0.025	-0.045
Rise	0.000	2.411	10.690	0.000	0.098	-0.186
Tilt	-0.140	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.025	0.098	0.000	0.025	0.007
Twist	0.000	-0.045	-0.186	0.000	0.007	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.398	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	2.593	0.693	0.000	0.022	-0.132
Rise	0.000	0.692	8.058	0.000	-0.124	-0.224
Tilt	-0.045	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.022	-0.124	0.000	0.018	0.013
Twist	0.000	-0.132	-0.224	0.000	0.013	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.672	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.332	1.209	0.000	0.008	-0.096
Rise	0.000	1.209	6.399	0.000	-0.067	-0.181
Tilt	-0.077	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.008	-0.067	0.000	0.015	0.008
Twist	0.000	-0.096	-0.181	0.000	0.008	0.027

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.10.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-catg.4.cte	0.000	0.000

	Mean	Stdev
-cgcg.11.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-ctag.8.cte	0.000	0.000

	Mean	Stdev
-gcgc.12.cte	0.000	0.000

	Mean	Stdev
-gcgc.3.cte	0.000	0.000

	Mean	Stdev
-gcgc.7.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
-tgca.6.cte	0.000	0.000

	Mean	Stdev
rise-10-ACGT	3.295	0.292

	Mean	Stdev
roll-10-ACGT	-1.498	5.799

	Mean	Stdev
shift-10-ACGT	0.000	0.978

	Mean	Stdev
slide-10-ACGT	-0.482	0.448

	Mean	Stdev
tilt-10-ACGT	0.000	4.053

	Mean	Stdev
twist-10-ACGT	30.968	5.187

	Mean	Stdev
rise-11-CGCG	3.300	0.347

	Mean	Stdev
roll-11-CGCG	5.061	6.710

	Mean	Stdev
shift-11-CGCG	0.000	0.994

	Mean	Stdev
slide-11-CGCG	0.050	0.583

	Mean	Stdev
tilt-11-CGCG	0.000	5.870

	Mean	Stdev
twist-11-CGCG	37.127	6.305

	Mean	Stdev
rise-12-GCGC	3.396	0.291

	Mean	Stdev
roll-12-GCGC	-1.546	5.676

	Mean	Stdev
shift-12-GCGC	0.000	0.894

	Mean	Stdev
slide-12-GCGC	-0.312	0.521

	Mean	Stdev
tilt-12-GCGC	0.000	4.613

	Mean	Stdev
twist-12-GCGC	32.240	5.713

	Mean	Stdev
rise-2-CGCG	3.266	0.340

	Mean	Stdev
roll-2-CGCG	5.232	6.542

	Mean	Stdev
shift-2-CGCG	0.000	1.014

	Mean	Stdev
slide-2-CGCG	-0.033	0.551

	Mean	Stdev
tilt-2-CGCG	0.000	5.933

	Mean	Stdev
twist-2-CGCG	35.545	6.605

	Mean	Stdev
rise-3-GCGC	3.394	0.292

	Mean	Stdev
roll-3-GCGC	-0.994	5.528

	Mean	Stdev
shift-3-GCGC	0.000	0.909

	Mean	Stdev
slide-3-GCGC	-0.322	0.516

	Mean	Stdev
tilt-3-GCGC	0.000	4.750

	Mean	Stdev
twist-3-GCGC	33.619	5.086

	Mean	Stdev
rise-4-CATG	3.332	0.324

	Mean	Stdev
roll-4-CATG	7.061	7.048

	Mean	Stdev
shift-4-CATG	0.000	1.139

	Mean	Stdev
slide-4-CATG	-0.131	0.568

	Mean	Stdev
tilt-4-CATG	0.000	5.671

	Mean	Stdev
twist-4-CATG	35.821	6.132

	Mean	Stdev
rise-5-ATAT	3.224	0.274

	Mean	Stdev
roll-5-ATAT	-0.678	5.258

	Mean	Stdev
shift-5-ATAT	0.000	0.659

	Mean	Stdev
slide-5-ATAT	-0.748	0.406

	Mean	Stdev
tilt-5-ATAT	0.000	3.600

	Mean	Stdev
twist-5-ATAT	30.124	4.426

	Mean	Stdev
rise-6-TGCA	3.402	0.344

	Mean	Stdev
roll-6-TGCA	7.519	6.947

	Mean	Stdev
shift-6-TGCA	0.000	1.124

	Mean	Stdev
slide-6-TGCA	-0.240	0.568

	Mean	Stdev
tilt-6-TGCA	0.000	5.485

	Mean	Stdev
twist-6-TGCA	35.213	5.907

	Mean	Stdev
rise-7-GCGC	3.470	0.285

	Mean	Stdev
roll-7-GCGC	-0.722	5.177

	Mean	Stdev
shift-7-GCGC	0.000	0.779

	Mean	Stdev
slide-7-GCGC	-0.663	0.574

	Mean	Stdev
tilt-7-GCGC	0.000	4.890

	Mean	Stdev
twist-7-GCGC	35.385	4.394

	Mean	Stdev
rise-8-CTAG	3.332	0.313

	Mean	Stdev
roll-8-CTAG	2.420	5.708

	Mean	Stdev
shift-8-CTAG	0.000	1.106

	Mean	Stdev
slide-8-CTAG	-0.353	0.652

	Mean	Stdev
tilt-8-CTAG	0.000	5.753

	Mean	Stdev
twist-8-CTAG	34.799	5.895

	Mean	Stdev
rise-9-TATA	3.142	0.304

	Mean	Stdev
roll-9-TATA	5.878	7.829

	Mean	Stdev
shift-9-TATA	0.000	1.366

	Mean	Stdev
slide-9-TATA	0.136	0.619

	Mean	Stdev
tilt-9-TATA	0.000	5.514

	Mean	Stdev
twist-9-TATA	33.657	7.168

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5'	G	-	C	-	G	-	C	-	A	-	T	-	G	-	C	-	T	-	A	-	C	-	G	-	C	-	G	3'
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3'	C	-	G	-	C	-	G	-	T	-	A	-	C	-	G	-	A	-	T	-	G	-	C	-	G	-	C	5'
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