NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	T	-	A	-	C	-	C	-	T	-	A	-	G	-	G	-	T	-	A	-	T	-	C	-	T	-	G	-	G	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	C	-	T	-	A	-	C	-	A	-	A	-	G	-	A	-	G	-	T	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.240	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.234	0.901	0.000	-0.082	-0.144
Rise	0.000	0.901	7.879	0.000	-0.047	-0.168
Tilt	-0.077	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.082	-0.047	0.000	0.023	0.009
Twist	0.000	-0.144	-0.168	0.000	0.009	0.034

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.559	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	3.644	2.807	0.000	-0.026	-0.106
Rise	0.000	2.807	9.514	0.000	0.055	-0.174
Tilt	-0.031	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.026	0.055	0.000	0.027	0.009
Twist	0.000	-0.106	-0.174	0.000	0.009	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.927	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	3.217	2.637	0.000	0.026	-0.101
Rise	0.000	2.637	9.346	0.000	0.093	-0.182
Tilt	-0.048	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.026	0.093	0.000	0.025	0.008
Twist	0.000	-0.101	-0.182	0.000	0.008	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.664	0.000	0.000	-0.073	0.000	0.000
Slide	0.000	2.022	1.506	0.000	-0.055	-0.092
Rise	0.000	1.506	7.712	0.000	-0.054	-0.197
Tilt	-0.073	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.055	-0.054	0.000	0.018	0.005
Twist	0.000	-0.092	-0.198	0.000	0.005	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.741	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	2.237	1.694	0.000	-0.058	-0.140
Rise	0.000	1.694	7.559	0.000	-0.004	-0.160
Tilt	-0.047	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.058	-0.004	0.000	0.018	0.005
Twist	0.000	-0.140	-0.160	0.000	0.005	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.499	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	4.692	3.269	0.000	-0.065	-0.088
Rise	0.000	3.269	11.286	0.000	0.079	-0.152
Tilt	-0.001	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.065	0.079	0.000	0.030	0.013
Twist	0.000	-0.088	-0.152	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.750	0.000	0.000	-0.010	0.000	0.000
Slide	0.000	4.927	3.044	0.000	-0.071	-0.081
Rise	0.000	3.044	11.274	0.000	0.072	-0.137
Tilt	-0.010	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.071	0.072	0.000	0.030	0.012
Twist	0.000	-0.081	-0.137	0.000	0.012	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.986	0.000	0.000	0.007	0.000	0.000
Slide	0.000	5.070	2.817	0.000	-0.080	-0.104
Rise	0.000	2.817	10.849	0.000	0.067	-0.141
Tilt	0.007	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.080	0.067	0.000	0.033	0.011
Twist	0.000	-0.104	-0.141	0.000	0.011	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.160	0.000	0.000	0.012	0.000	0.000
Slide	0.000	4.406	2.812	0.000	-0.034	-0.070
Rise	0.000	2.812	11.082	0.000	0.096	-0.137
Tilt	0.012	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.034	0.096	0.000	0.027	0.009
Twist	0.000	-0.070	-0.137	0.000	0.009	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.684	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	2.668	0.540	0.000	0.011	-0.113
Rise	0.000	0.540	8.014	0.000	-0.109	-0.235
Tilt	-0.062	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.011	-0.109	0.000	0.019	0.011
Twist	0.000	-0.113	-0.235	0.000	0.011	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.709	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.162	1.455	0.000	0.013	-0.100
Rise	0.000	1.455	5.984	0.000	-0.052	-0.163
Tilt	-0.082	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.013	-0.052	0.000	0.015	0.006
Twist	0.000	-0.100	-0.163	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.647	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.374	0.690	0.000	0.013	-0.102
Rise	0.000	0.690	6.865	0.000	-0.072	-0.184
Tilt	-0.077	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.013	-0.072	0.000	0.016	0.009
Twist	0.000	-0.102	-0.184	0.000	0.009	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.858	0.000	0.000	-0.109	0.000	0.000
Slide	0.000	1.584	1.929	0.000	-0.022	-0.113
Rise	0.000	1.929	7.696	0.000	-0.016	-0.153
Tilt	-0.109	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.022	-0.016	0.000	0.019	0.006
Twist	0.000	-0.113	-0.153	0.000	0.006	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.769	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	1.895	1.793	0.000	-0.016	-0.133
Rise	0.000	1.793	7.309	0.000	0.000	-0.150
Tilt	-0.080	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.016	0.000	0.000	0.018	0.003
Twist	0.000	-0.133	-0.150	0.000	0.003	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.864	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	1.954	1.567	0.000	-0.053	-0.081
Rise	0.000	1.567	6.492	0.000	-0.049	-0.190
Tilt	-0.045	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.053	-0.049	0.000	0.017	0.005
Twist	0.000	-0.081	-0.190	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.048	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	1.987	1.381	0.000	-0.022	-0.131
Rise	0.000	1.381	7.501	0.000	-0.047	-0.168
Tilt	-0.067	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.022	-0.047	0.000	0.021	0.008
Twist	0.000	-0.131	-0.168	0.000	0.008	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.827	0.000	0.000	-0.034	0.000	0.000
Slide	0.000	1.992	1.416	0.000	-0.024	-0.112
Rise	0.000	1.416	7.295	0.000	-0.058	-0.180
Tilt	-0.034	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.024	-0.058	0.000	0.018	0.006
Twist	0.000	-0.112	-0.180	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.865	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	1.968	1.393	0.000	-0.048	-0.105
Rise	0.000	1.393	8.087	0.000	-0.032	-0.122
Tilt	-0.104	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.048	-0.032	0.000	0.022	0.011
Twist	0.000	-0.105	-0.122	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.061	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	2.224	1.594	0.000	-0.019	-0.137
Rise	0.000	1.594	7.449	0.000	-0.031	-0.150
Tilt	-0.081	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.019	-0.031	0.000	0.020	0.007
Twist	0.000	-0.137	-0.150	0.000	0.007	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.758	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	1.611	1.519	0.000	-0.019	-0.108
Rise	0.000	1.519	7.620	0.000	-0.035	-0.170
Tilt	-0.083	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.019	-0.035	0.000	0.018	0.006
Twist	0.000	-0.108	-0.170	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.768	0.000	0.000	-0.046	0.000	0.000
Slide	0.000	3.079	2.557	0.000	0.027	-0.093
Rise	0.000	2.557	9.832	0.000	0.090	-0.210
Tilt	-0.046	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.027	0.090	0.000	0.024	0.008
Twist	0.000	-0.093	-0.210	0.000	0.008	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.439	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	2.054	1.128	0.000	0.010	-0.122
Rise	0.000	1.128	7.506	0.000	-0.075	-0.218
Tilt	-0.049	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.010	-0.075	0.000	0.014	0.010
Twist	0.000	-0.122	-0.218	0.000	0.010	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.861	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	1.956	1.367	0.000	-0.007	-0.121
Rise	0.000	1.367	6.851	0.000	-0.030	-0.166
Tilt	-0.052	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.007	-0.030	0.000	0.018	0.006
Twist	0.000	-0.121	-0.166	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.731	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.292	0.690	0.000	-0.039	-0.116
Rise	0.000	0.690	6.411	0.000	-0.042	-0.121
Tilt	-0.075	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.039	-0.042	0.000	0.021	0.010
Twist	0.000	-0.116	-0.121	0.000	0.010	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.102	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	2.089	1.409	0.000	-0.019	-0.127
Rise	0.000	1.409	7.453	0.000	-0.034	-0.156
Tilt	-0.053	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.019	-0.034	0.000	0.020	0.008
Twist	0.000	-0.127	-0.156	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.430	0.000	0.000	-0.005	0.000	0.000
Slide	0.000	0.984	0.453	0.000	0.036	-0.057
Rise	0.000	0.453	1.020	0.000	-0.001	-0.052
Tilt	-0.005	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.036	-0.001	0.000	0.005	-0.003
Twist	0.000	-0.057	-0.052	0.000	-0.003	0.008

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.632	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.419	1.064	0.000	0.008	-0.096
Rise	0.000	1.064	6.722	0.000	-0.070	-0.179
Tilt	-0.090	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.008	-0.070	0.000	0.016	0.008
Twist	0.000	-0.096	-0.179	0.000	0.008	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.634	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.207	1.593	0.000	0.017	-0.096
Rise	0.000	1.593	6.381	0.000	-0.066	-0.192
Tilt	-0.075	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.017	-0.066	0.000	0.015	0.005
Twist	0.000	-0.097	-0.192	0.000	0.005	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.437	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.671	1.165	0.000	-0.071	-0.150
Rise	0.000	1.165	7.904	0.000	-0.053	-0.181
Tilt	-0.061	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.071	-0.053	0.000	0.023	0.008
Twist	0.000	-0.150	-0.181	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.18.cte	0.000	0.000

	Mean	Stdev
-acgt.13.cte	0.000	0.000

	Mean	Stdev
-acgt.19.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.21.cte	0.000	0.000

	Mean	Stdev
-atat.24.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-atat.9.cte	0.000	0.000

	Mean	Stdev
-catg.15.cte	0.000	0.000

	Mean	Stdev
-catg.20.cte	0.000	0.000

	Mean	Stdev
-catg.8.cte	0.000	0.000

	Mean	Stdev
-ccgg.14.cte	0.000	0.000

	Mean	Stdev
-ccgg.7.cte	0.000	0.000

	Mean	Stdev
-ctag.29.cte	0.000	0.000

	Mean	Stdev
-gatc.12.cte	0.000	0.000

	Mean	Stdev
-gatc.17.cte	0.000	0.000

	Mean	Stdev
-gatc.23.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-ggcc.3.cte	0.000	0.000

	Mean	Stdev
-gtac.26.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tcga.28.cte	0.000	0.000

	Mean	Stdev
-tcga.30.cte	0.000	0.000

	Mean	Stdev
-tcga.6.cte	0.000	0.000

	Mean	Stdev
-tgca.2.cte	0.000	0.000

	Mean	Stdev
-tgca.22.cte	0.000	0.000

	Mean	Stdev
-tgca.25.cte	0.000	0.000

	Mean	Stdev
-ttaa.27.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.237	0.317

	Mean	Stdev
roll-10-TATA	4.220	8.102

	Mean	Stdev
shift-10-TATA	0.000	1.315

	Mean	Stdev
slide-10-TATA	0.172	0.676

	Mean	Stdev
tilt-10-TATA	0.000	5.662

	Mean	Stdev
twist-10-TATA	36.651	6.866

	Mean	Stdev
rise-11-AGCT	3.289	0.321

	Mean	Stdev
roll-11-AGCT	3.720	5.974

	Mean	Stdev
shift-11-AGCT	0.000	1.082

	Mean	Stdev
slide-11-AGCT	-0.270	0.625

	Mean	Stdev
tilt-11-AGCT	0.000	4.863

	Mean	Stdev
twist-11-AGCT	32.385	6.092

	Mean	Stdev
rise-12-GATC	3.447	0.305

	Mean	Stdev
roll-12-GATC	0.684	5.467

	Mean	Stdev
shift-12-GATC	0.000	0.802

	Mean	Stdev
slide-12-GATC	-0.270	0.648

	Mean	Stdev
tilt-12-GATC	0.000	4.563

	Mean	Stdev
twist-12-GATC	37.737	5.055

	Mean	Stdev
rise-13-ACGT	3.420	0.295

	Mean	Stdev
roll-13-ACGT	-0.500	4.979

	Mean	Stdev
shift-13-ACGT	0.000	0.618

	Mean	Stdev
slide-13-ACGT	-0.878	0.466

	Mean	Stdev
tilt-13-ACGT	0.000	4.012

	Mean	Stdev
twist-13-ACGT	32.681	4.303

	Mean	Stdev
rise-14-CCGG	3.494	0.332

	Mean	Stdev
roll-14-CCGG	3.751	5.760

	Mean	Stdev
shift-14-CCGG	0.000	1.046

	Mean	Stdev
slide-14-CCGG	-0.383	0.809

	Mean	Stdev
tilt-14-CCGG	0.000	5.042

	Mean	Stdev
twist-14-CCGG	35.557	5.281

	Mean	Stdev
rise-15-CATG	3.083	0.317

	Mean	Stdev
roll-15-CATG	6.843	6.796

	Mean	Stdev
shift-15-CATG	0.000	1.047

	Mean	Stdev
slide-15-CATG	-0.074	0.552

	Mean	Stdev
tilt-15-CATG	0.000	5.361

	Mean	Stdev
twist-15-CATG	31.121	7.003

	Mean	Stdev
rise-16-AGCT	3.276	0.314

	Mean	Stdev
roll-16-AGCT	1.078	6.083

	Mean	Stdev
shift-16-AGCT	0.000	0.938

	Mean	Stdev
slide-16-AGCT	-0.551	0.691

	Mean	Stdev
tilt-16-AGCT	0.000	4.729

	Mean	Stdev
twist-16-AGCT	31.121	6.650

	Mean	Stdev
rise-17-GATC	3.436	0.320

	Mean	Stdev
roll-17-GATC	0.851	5.868

	Mean	Stdev
shift-17-GATC	0.000	0.865

	Mean	Stdev
slide-17-GATC	-0.308	0.642

	Mean	Stdev
tilt-17-GATC	0.000	4.749

	Mean	Stdev
twist-17-GATC	35.528	6.043

	Mean	Stdev
rise-18-AATT	3.293	0.289

	Mean	Stdev
roll-18-AATT	1.251	5.451

	Mean	Stdev
shift-18-AATT	0.000	0.754

	Mean	Stdev
slide-18-AATT	-0.359	0.619

	Mean	Stdev
tilt-18-AATT	0.000	4.986

	Mean	Stdev
twist-18-AATT	34.981	5.082

	Mean	Stdev
rise-19-ACGT	3.397	0.301

	Mean	Stdev
roll-19-ACGT	-0.941	5.259

	Mean	Stdev
shift-19-ACGT	0.000	0.820

	Mean	Stdev
slide-19-ACGT	-0.778	0.495

	Mean	Stdev
tilt-19-ACGT	0.000	3.843

	Mean	Stdev
twist-19-ACGT	32.921	4.408

	Mean	Stdev
rise-2-TGCA	3.377	1.087

	Mean	Stdev
roll-2-TGCA	10.679	14.847

	Mean	Stdev
shift-2-TGCA	0.000	1.295

	Mean	Stdev
slide-2-TGCA	0.549	1.112

	Mean	Stdev
tilt-2-TGCA	0.664	48.118

	Mean	Stdev
twist-2-TGCA	44.441	15.139

	Mean	Stdev
rise-20-CATG	3.491	0.361

	Mean	Stdev
roll-20-CATG	6.752	6.868

	Mean	Stdev
shift-20-CATG	0.000	1.113

	Mean	Stdev
slide-20-CATG	-0.259	0.619

	Mean	Stdev
tilt-20-CATG	0.000	5.550

	Mean	Stdev
twist-20-CATG	38.071	5.778

	Mean	Stdev
rise-21-ATAT	3.179	0.272

	Mean	Stdev
roll-21-ATAT	-1.197	5.062

	Mean	Stdev
shift-21-ATAT	0.000	0.625

	Mean	Stdev
slide-21-ATAT	-0.748	0.410

	Mean	Stdev
tilt-21-ATAT	0.000	3.679

	Mean	Stdev
twist-21-ATAT	29.543	4.202

	Mean	Stdev
rise-22-TGCA	3.315	0.330

	Mean	Stdev
roll-22-TGCA	7.332	6.766

	Mean	Stdev
shift-22-TGCA	0.000	1.338

	Mean	Stdev
slide-22-TGCA	0.013	0.550

	Mean	Stdev
tilt-22-TGCA	0.000	6.518

	Mean	Stdev
twist-22-TGCA	34.290	5.865

	Mean	Stdev
rise-23-GATC	3.351	0.289

	Mean	Stdev
roll-23-GATC	1.475	5.581

	Mean	Stdev
shift-23-GATC	0.000	1.059

	Mean	Stdev
slide-23-GATC	0.016	0.623

	Mean	Stdev
tilt-23-GATC	0.000	5.514

	Mean	Stdev
twist-23-GATC	37.289	4.635

	Mean	Stdev
rise-24-ATAT	3.243	0.263

	Mean	Stdev
roll-24-ATAT	-2.138	4.894

	Mean	Stdev
shift-24-ATAT	0.000	0.579

	Mean	Stdev
slide-24-ATAT	-0.831	0.391

	Mean	Stdev
tilt-24-ATAT	0.000	3.664

	Mean	Stdev
twist-24-ATAT	30.771	4.149

	Mean	Stdev
rise-25-TGCA	3.490	0.364

	Mean	Stdev
roll-25-TGCA	7.500	6.726

	Mean	Stdev
shift-25-TGCA	0.000	1.167

	Mean	Stdev
slide-25-TGCA	-0.248	0.609

	Mean	Stdev
tilt-25-TGCA	0.000	5.608

	Mean	Stdev
twist-25-TGCA	37.035	5.550

	Mean	Stdev
rise-26-GTAC	3.352	0.294

	Mean	Stdev
roll-26-GTAC	-0.822	5.348

	Mean	Stdev
shift-26-GTAC	0.000	0.905

	Mean	Stdev
slide-26-GTAC	-0.705	0.498

	Mean	Stdev
tilt-26-GTAC	0.000	3.881

	Mean	Stdev
twist-26-GTAC	32.583	4.296

	Mean	Stdev
rise-27-TTAA	3.276	0.293

	Mean	Stdev
roll-27-TTAA	0.742	5.337

	Mean	Stdev
shift-27-TTAA	0.000	0.668

	Mean	Stdev
slide-27-TTAA	-0.510	0.555

	Mean	Stdev
tilt-27-TTAA	0.000	4.626

	Mean	Stdev
twist-27-TTAA	34.559	5.218

	Mean	Stdev
rise-28-TCGA	3.466	0.325

	Mean	Stdev
roll-28-TCGA	0.956	5.938

	Mean	Stdev
shift-28-TCGA	0.000	0.871

	Mean	Stdev
slide-28-TCGA	-0.275	0.670

	Mean	Stdev
tilt-28-TCGA	0.000	4.658

	Mean	Stdev
twist-28-TCGA	36.605	6.094

	Mean	Stdev
rise-29-CTAG	3.366	0.360

	Mean	Stdev
roll-29-CTAG	2.949	6.172

	Mean	Stdev
shift-29-CTAG	0.000	0.854

	Mean	Stdev
slide-29-CTAG	-0.601	0.638

	Mean	Stdev
tilt-29-CTAG	0.000	4.354

	Mean	Stdev
twist-29-CTAG	31.611	5.648

	Mean	Stdev
rise-3-GGCC	3.440	0.314

	Mean	Stdev
roll-3-GGCC	4.461	5.993

	Mean	Stdev
shift-3-GGCC	0.000	1.012

	Mean	Stdev
slide-3-GGCC	-0.297	0.746

	Mean	Stdev
tilt-3-GGCC	0.000	4.572

	Mean	Stdev
twist-3-GGCC	32.940	6.007

	Mean	Stdev
rise-30-TCGA	3.334	0.316

	Mean	Stdev
roll-30-TCGA	2.028	5.731

	Mean	Stdev
shift-30-TCGA	0.000	1.051

	Mean	Stdev
slide-30-TCGA	0.144	0.570

	Mean	Stdev
tilt-30-TCGA	0.000	5.361

	Mean	Stdev
twist-30-TCGA	36.250	5.669

	Mean	Stdev
rise-4-GATC	3.463	0.307

	Mean	Stdev
roll-4-GATC	0.843	5.618

	Mean	Stdev
shift-4-GATC	0.000	0.825

	Mean	Stdev
slide-4-GATC	-0.399	0.618

	Mean	Stdev
tilt-4-GATC	0.000	4.725

	Mean	Stdev
twist-4-GATC	37.748	4.928

	Mean	Stdev
rise-5-ATAT	3.248	0.262

	Mean	Stdev
roll-5-ATAT	-1.736	4.592

	Mean	Stdev
shift-5-ATAT	0.000	0.543

	Mean	Stdev
slide-5-ATAT	-0.842	0.382

	Mean	Stdev
tilt-5-ATAT	0.000	3.591

	Mean	Stdev
twist-5-ATAT	31.015	3.807

	Mean	Stdev
rise-6-TCGA	3.439	0.305

	Mean	Stdev
roll-6-TCGA	0.984	5.769

	Mean	Stdev
shift-6-TCGA	0.000	0.764

	Mean	Stdev
slide-6-TCGA	-0.483	0.622

	Mean	Stdev
tilt-6-TCGA	0.000	4.652

	Mean	Stdev
twist-6-TCGA	37.862	4.948

	Mean	Stdev
rise-7-CCGG	3.447	0.325

	Mean	Stdev
roll-7-CCGG	3.577	5.851

	Mean	Stdev
shift-7-CCGG	0.000	0.997

	Mean	Stdev
slide-7-CCGG	-0.602	0.777

	Mean	Stdev
tilt-7-CCGG	0.000	4.659

	Mean	Stdev
twist-7-CCGG	30.481	6.827

	Mean	Stdev
rise-8-CATG	3.227	0.323

	Mean	Stdev
roll-8-CATG	6.654	6.933

	Mean	Stdev
shift-8-CATG	0.000	1.181

	Mean	Stdev
slide-8-CATG	-0.026	0.562

	Mean	Stdev
tilt-8-CATG	0.000	5.915

	Mean	Stdev
twist-8-CATG	34.658	5.971

	Mean	Stdev
rise-9-ATAT	3.207	0.267

	Mean	Stdev
roll-9-ATAT	-1.449	5.155

	Mean	Stdev
shift-9-ATAT	0.000	0.712

	Mean	Stdev
slide-9-ATAT	-0.712	0.406

	Mean	Stdev
tilt-9-ATAT	0.000	3.691

	Mean	Stdev
twist-9-ATAT	29.628	4.543

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21		22		23		24		25		26		27		28		29		30		31		32
5'	A	-	T	-	G	-	G	-	A	-	T	-	C	-	C	-	A	-	T	-	A	-	G	-	A	-	C	-	C	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	G	-	A	-	T	-	G	-	T	-	T	-	C	-	T	-	C	-	A	3'
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3'	T	-	A	-	C	-	C	-	T	-	A	-	G	-	G	-	T	-	A	-	T	-	C	-	T	-	G	-	G	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	C	-	T	-	A	-	C	-	A	-	A	-	G	-	A	-	G	-	T	5'
	64		63		62		61		60		59		58		57		56		55		54		53		52		51		50		49		48		47		46		45		44		43		42		41		40		39		38		37		36		35		34		33

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5'	A	-	T	-	G	-	G	-	A	-	T	-	C	-	C	-	A	-	T	-	A	-	G	-	A	-	C	-	C	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	G	-	A	-	T	-	G	-	T	-	T	-	C	-	T	-	C	-	A	3'
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3'	T	-	A	-	C	-	C	-	T	-	A	-	G	-	G	-	T	-	A	-	T	-	C	-	T	-	G	-	G	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	C	-	T	-	A	-	C	-	A	-	A	-	G	-	A	-	G	-	T	5'
	64		63		62		61		60		59		58		57		56		55		54		53		52		51		50		49		48		47		46		45		44		43		42		41		40		39		38		37		36		35		34		33

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5'	A	-	T	-	G	-	G	-	A	-	T	-	C	-	C	-	A	-	T	-	A	-	G	-	A	-	C	-	C	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	G	-	A	-	T	-	G	-	T	-	T	-	C	-	T	-	C	-	A	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	T	-	A	-	C	-	C	-	T	-	A	-	G	-	G	-	T	-	A	-	T	-	C	-	T	-	G	-	G	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	C	-	T	-	A	-	C	-	A	-	A	-	G	-	A	-	G	-	T	5'
	64		63		62		61		60		59		58		57		56		55		54		53		52		51		50		49		48		47		46		45		44		43		42		41		40		39		38		37		36		35		34		33