NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	A	-	T	-	A	-	C	-	A	-	A	-	T	-	A	-	C	-	G	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	T	-	A	-	T	-	G	-	A	-	T	-	A	-	T	-	G	-	C	-	C	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.216	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	1.697	0.545	0.000	-0.019	-0.101
Rise	0.000	0.545	5.664	0.000	-0.013	-0.120
Tilt	-0.011	0.000	0.000	0.011	0.000	0.000
Roll	0.000	-0.019	-0.013	0.000	0.011	0.003
Twist	0.000	-0.101	-0.120	0.000	0.003	0.011

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.748	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	3.884	2.581	0.000	0.024	-0.085
Rise	0.000	2.581	10.660	0.000	0.084	-0.257
Tilt	-0.056	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.024	0.084	0.000	0.025	0.012
Twist	0.000	-0.085	-0.257	0.000	0.012	0.034

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.048	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	3.098	2.502	0.000	0.016	-0.054
Rise	0.000	2.502	9.600	0.000	0.076	-0.181
Tilt	-0.029	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.016	0.076	0.000	0.024	0.009
Twist	0.000	-0.054	-0.181	0.000	0.009	0.028

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.177	0.000	0.000	0.018	0.000	0.000
Slide	0.000	4.700	2.622	0.000	-0.044	-0.052
Rise	0.000	2.622	11.361	0.000	0.087	-0.134
Tilt	0.018	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.044	0.087	0.000	0.028	0.009
Twist	0.000	-0.052	-0.134	0.000	0.009	0.030

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.388	0.000	0.000	0.001	0.000	0.000
Slide	0.000	4.607	2.438	0.000	-0.045	-0.114
Rise	0.000	2.438	10.126	0.000	0.059	-0.129
Tilt	0.001	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.045	0.059	0.000	0.027	0.011
Twist	0.000	-0.114	-0.129	0.000	0.011	0.043

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.166	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	1.336	0.147	0.000	-0.027	-0.082
Rise	0.000	0.146	4.283	0.000	-0.004	-0.070
Tilt	-0.002	0.000	0.000	0.011	0.000	0.000
Roll	0.000	-0.027	-0.004	0.000	0.009	0.003
Twist	0.000	-0.082	-0.070	0.000	0.003	0.009

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.776	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.353	0.870	0.000	0.011	-0.123
Rise	0.000	0.870	6.434	0.000	-0.075	-0.180
Tilt	-0.088	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.011	-0.075	0.000	0.015	0.006
Twist	0.000	-0.123	-0.180	0.000	0.006	0.028

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.029	0.000	0.000	-0.127	0.000	0.000
Slide	0.000	2.792	1.062	0.000	0.006	-0.128
Rise	0.000	1.062	6.765	0.000	-0.050	-0.185
Tilt	-0.127	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.006	-0.050	0.000	0.014	0.004
Twist	0.000	-0.128	-0.185	0.000	0.004	0.029

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.507	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	1.856	1.144	0.000	0.017	-0.100
Rise	0.000	1.144	7.564	0.000	-0.087	-0.218
Tilt	-0.053	0.000	0.000	0.022	0.000	0.000
Roll	0.000	0.017	-0.087	0.000	0.015	0.009
Twist	0.000	-0.100	-0.218	0.000	0.009	0.029

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.439	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	2.295	0.972	0.000	0.030	-0.105
Rise	0.000	0.972	7.548	0.000	-0.083	-0.216
Tilt	-0.051	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.030	-0.083	0.000	0.015	0.009
Twist	0.000	-0.105	-0.216	0.000	0.009	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-aata.7.cte	0.000	0.000

	Mean	Stdev
-acgt.10.cte	0.000	0.000

	Mean	Stdev
-acgt.5.cte	0.000	0.000

	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-caag.6.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.11.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
rise-10-ACGT	3.224	0.298

	Mean	Stdev
roll-10-ACGT	-0.568	5.928

	Mean	Stdev
shift-10-ACGT	0.000	0.939

	Mean	Stdev
slide-10-ACGT	-0.404	0.426

	Mean	Stdev
tilt-10-ACGT	0.000	4.184

	Mean	Stdev
twist-10-ACGT	30.127	5.515

	Mean	Stdev
rise-11-CGCG	3.276	0.327

	Mean	Stdev
roll-11-CGCG	3.925	6.808

	Mean	Stdev
shift-11-CGCG	0.000	1.095

	Mean	Stdev
slide-11-CGCG	0.235	0.519

	Mean	Stdev
tilt-11-CGCG	0.000	6.425

	Mean	Stdev
twist-11-CGCG	37.247	5.455

	Mean	Stdev
rise-2-CATG	3.321	0.337

	Mean	Stdev
roll-2-CATG	6.946	6.915

	Mean	Stdev
shift-2-CATG	0.000	1.113

	Mean	Stdev
slide-2-CATG	-0.067	0.588

	Mean	Stdev
tilt-2-CATG	0.000	6.150

	Mean	Stdev
twist-2-CATG	35.486	5.947

	Mean	Stdev
rise-3-ATAT	3.161	0.259

	Mean	Stdev
roll-3-ATAT	-0.884	5.021

	Mean	Stdev
shift-3-ATAT	0.000	0.708

	Mean	Stdev
slide-3-ATAT	-0.629	0.386

	Mean	Stdev
tilt-3-ATAT	0.000	3.696

	Mean	Stdev
twist-3-ATAT	28.093	4.900

	Mean	Stdev
rise-4-TATA	3.310	0.319

	Mean	Stdev
roll-4-TATA	3.484	7.534

	Mean	Stdev
shift-4-TATA	0.000	1.237

	Mean	Stdev
slide-4-TATA	0.118	0.677

	Mean	Stdev
tilt-4-TATA	0.000	5.888

	Mean	Stdev
twist-4-TATA	38.727	6.132

	Mean	Stdev
rise-5-ACGT	3.291	0.306

	Mean	Stdev
roll-5-ACGT	-0.243	5.625

	Mean	Stdev
shift-5-ACGT	0.000	0.757

	Mean	Stdev
slide-5-ACGT	-0.585	0.490

	Mean	Stdev
tilt-5-ACGT	0.000	4.307

	Mean	Stdev
twist-5-ACGT	30.468	5.478

	Mean	Stdev
rise-6-CAAG	3.202	0.419

	Mean	Stdev
roll-6-CAAG	8.724	8.450

	Mean	Stdev
shift-6-CAAG	0.000	1.879

	Mean	Stdev
slide-6-CAAG	-0.154	1.039

	Mean	Stdev
tilt-6-CAAG	0.000	7.340

	Mean	Stdev
twist-6-CAAG	29.560	13.753

	Mean	Stdev
rise-7-AATA	3.442	0.393

	Mean	Stdev
roll-7-AATA	2.852	7.841

	Mean	Stdev
shift-7-AATA	0.000	1.686

	Mean	Stdev
slide-7-AATA	0.214	0.949

	Mean	Stdev
tilt-7-AATA	0.000	7.328

	Mean	Stdev
twist-7-AATA	39.186	13.886

	Mean	Stdev
rise-8-ATAT	3.252	0.266

	Mean	Stdev
roll-8-ATAT	-1.813	5.050

	Mean	Stdev
shift-8-ATAT	0.000	0.650

	Mean	Stdev
slide-8-ATAT	-0.813	0.393

	Mean	Stdev
tilt-8-ATAT	0.000	3.943

	Mean	Stdev
twist-8-ATAT	32.153	4.101

	Mean	Stdev
rise-9-TATA	3.342	0.314

	Mean	Stdev
roll-9-TATA	2.495	7.502

	Mean	Stdev
shift-9-TATA	0.000	1.318

	Mean	Stdev
slide-9-TATA	0.182	0.597

	Mean	Stdev
tilt-9-TATA	0.000	5.492

	Mean	Stdev
twist-9-TATA	39.520	5.735

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	A	-	T	-	A	-	C	-	A	-	A	-	T	-	A	-	C	-	G	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	T	-	A	-	T	-	G	-	A	-	T	-	A	-	T	-	G	-	C	-	C	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	A	-	T	-	A	-	C	-	A	-	A	-	T	-	A	-	C	-	G	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	T	-	A	-	T	-	G	-	A	-	T	-	A	-	T	-	G	-	C	-	C	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	A	-	T	-	A	-	C	-	A	-	A	-	T	-	A	-	C	-	G	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	T	-	A	-	T	-	G	-	A	-	T	-	A	-	T	-	G	-	C	-	C	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14