NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.733	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	2.458	0.735	0.000	-0.051	-0.110
Rise	0.000	0.735	6.125	0.000	-0.020	-0.133
Tilt	-0.044	0.000	0.000	0.017	0.000	0.000
Roll	0.000	-0.051	-0.020	0.000	0.019	0.011
Twist	0.000	-0.110	-0.133	0.000	0.011	0.043

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.066	0.000	0.000	0.020	0.000	0.000
Slide	0.000	3.136	1.196	0.000	0.003	-0.082
Rise	0.000	1.196	6.280	0.000	0.040	-0.152
Tilt	0.020	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.003	0.040	0.000	0.017	0.008
Twist	0.000	-0.082	-0.152	0.000	0.008	0.048

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.181	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	1.212	0.109	0.000	0.036	0.009
Rise	0.000	0.109	1.239	0.000	-0.005	0.050
Tilt	-0.047	0.000	0.000	0.019	0.000	0.000
Roll	0.000	0.036	-0.005	0.000	0.009	0.000
Twist	0.000	0.009	0.050	0.000	0.000	0.008

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.462	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	1.349	-0.117	0.000	0.042	0.009
Rise	0.000	-0.117	6.380	0.000	-0.116	-0.201
Tilt	-0.081	0.000	0.000	0.018	0.000	0.000
Roll	0.000	0.042	-0.116	0.000	0.012	0.007
Twist	0.000	0.009	-0.201	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.503	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	1.449	0.930	0.000	-0.014	0.001
Rise	0.000	0.930	5.456	0.000	-0.092	-0.138
Tilt	-0.074	0.000	0.000	0.020	0.000	0.000
Roll	0.000	-0.014	-0.092	0.000	0.021	0.013
Twist	0.000	0.001	-0.138	0.000	0.013	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.225	0.000	0.000	0.006	0.000	0.000
Slide	0.000	0.347	-0.228	0.000	-0.011	-0.006
Rise	0.000	-0.228	0.661	0.000	0.020	-0.001
Tilt	0.006	0.000	0.000	0.006	0.000	0.000
Roll	0.000	-0.011	0.020	0.000	0.004	0.001
Twist	0.000	-0.006	-0.001	0.000	0.001	0.003

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.056	0.000	0.000	0.005	0.000	0.000
Slide	0.000	0.087	-0.043	0.000	-0.008	-0.004
Rise	0.000	-0.043	0.158	0.000	0.018	-0.000
Tilt	0.005	0.000	0.000	0.001	0.000	0.000
Roll	0.000	-0.008	0.018	0.000	0.004	0.000
Twist	0.000	-0.004	-0.000	0.000	0.000	0.001

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.557	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	1.666	0.946	0.000	-0.018	-0.021
Rise	0.000	0.946	5.826	0.000	-0.073	-0.151
Tilt	-0.061	0.000	0.000	0.024	0.000	0.000
Roll	0.000	-0.018	-0.073	0.000	0.020	0.013
Twist	0.000	-0.021	-0.151	0.000	0.013	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.941	0.000	0.000	-0.040	0.000	0.000
Slide	0.000	2.962	1.166	0.000	-0.042	-0.138
Rise	0.000	1.166	6.082	0.000	-0.029	-0.178
Tilt	-0.040	0.000	0.000	0.016	0.000	0.000
Roll	0.000	-0.042	-0.029	0.000	0.020	0.011
Twist	0.000	-0.138	-0.178	0.000	0.011	0.048

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-atat.6.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.7.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.463	1.152

	Mean	Stdev
roll-10-GCGC	3.676	13.401

	Mean	Stdev
shift-10-GCGC	0.000	1.633

	Mean	Stdev
slide-10-GCGC	0.432	1.525

	Mean	Stdev
tilt-10-GCGC	0.000	10.197

	Mean	Stdev
twist-10-GCGC	31.115	16.103

	Mean	Stdev
rise-2-GCGC	5.545	3.019

	Mean	Stdev
roll-2-GCGC	-0.127	17.996

	Mean	Stdev
shift-2-GCGC	0.000	4.184

	Mean	Stdev
slide-2-GCGC	4.267	3.658

	Mean	Stdev
tilt-2-GCGC	0.000	27.662

	Mean	Stdev
twist-2-GCGC	47.662	44.141

	Mean	Stdev
rise-3-CGCG	3.532	0.797

	Mean	Stdev
roll-3-CGCG	5.762	8.617

	Mean	Stdev
shift-3-CGCG	0.000	0.742

	Mean	Stdev
slide-3-CGCG	1.175	0.745

	Mean	Stdev
tilt-3-CGCG	0.000	5.882

	Mean	Stdev
twist-3-CGCG	26.331	9.732

	Mean	Stdev
rise-4-GATC	3.325	0.382

	Mean	Stdev
roll-4-GATC	5.669	6.397

	Mean	Stdev
shift-4-GATC	0.000	0.691

	Mean	Stdev
slide-4-GATC	0.792	0.685

	Mean	Stdev
tilt-4-GATC	0.000	5.999

	Mean	Stdev
twist-4-GATC	32.607	6.571

	Mean	Stdev
rise-5-AATT	3.415	0.323

	Mean	Stdev
roll-5-AATT	6.792	6.042

	Mean	Stdev
shift-5-AATT	0.000	0.602

	Mean	Stdev
slide-5-AATT	0.017	0.527

	Mean	Stdev
tilt-5-AATT	0.000	6.136

	Mean	Stdev
twist-5-AATT	35.779	4.267

	Mean	Stdev
rise-6-ATAT	3.371	0.333

	Mean	Stdev
roll-6-ATAT	4.505	6.166

	Mean	Stdev
shift-6-ATAT	0.000	0.747

	Mean	Stdev
slide-6-ATAT	-0.527	0.454

	Mean	Stdev
tilt-6-ATAT	0.000	4.504

	Mean	Stdev
twist-6-ATAT	32.318	3.884

	Mean	Stdev
rise-7-TTAA	3.402	0.334

	Mean	Stdev
roll-7-TTAA	6.929	5.810

	Mean	Stdev
shift-7-TTAA	0.000	0.565

	Mean	Stdev
slide-7-TTAA	0.053	0.486

	Mean	Stdev
tilt-7-TTAA	0.000	6.276

	Mean	Stdev
twist-7-TTAA	35.808	4.096

	Mean	Stdev
rise-8-TCGA	3.390	0.357

	Mean	Stdev
roll-8-TCGA	4.211	6.304

	Mean	Stdev
shift-8-TCGA	0.000	0.647

	Mean	Stdev
slide-8-TCGA	0.554	0.623

	Mean	Stdev
tilt-8-TCGA	0.000	5.221

	Mean	Stdev
twist-8-TCGA	35.134	5.558

	Mean	Stdev
rise-9-CGCG	3.343	0.370

	Mean	Stdev
roll-9-CGCG	9.203	8.290

	Mean	Stdev
shift-9-CGCG	0.000	0.731

	Mean	Stdev
slide-9-CGCG	0.810	0.703

	Mean	Stdev
tilt-9-CGCG	0.000	6.594

	Mean	Stdev
twist-9-CGCG	32.517	6.191

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