NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.978	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	3.649	2.552	0.000	0.014	-0.120
Rise	0.000	2.552	10.120	0.000	0.049	-0.220
Tilt	-0.069	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.014	0.049	0.000	0.026	0.012
Twist	0.000	-0.120	-0.220	0.000	0.012	0.041

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.216	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	3.936	3.093	0.000	-0.027	-0.095
Rise	0.000	3.093	11.211	0.000	0.109	-0.201
Tilt	-0.043	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.027	0.109	0.000	0.027	0.005
Twist	0.000	-0.095	-0.201	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.213	0.000	0.000	-0.021	0.000	0.000
Slide	0.000	4.544	2.691	0.000	-0.035	-0.140
Rise	0.000	2.691	11.178	0.000	0.074	-0.162
Tilt	-0.021	0.000	0.000	0.049	0.000	0.000
Roll	0.000	-0.035	0.074	0.000	0.028	0.014
Twist	0.000	-0.140	-0.162	0.000	0.014	0.055

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.433	0.000	0.000	0.010	0.000	0.000
Slide	0.000	4.524	2.670	0.000	-0.049	-0.035
Rise	0.000	2.670	10.202	0.000	0.048	-0.108
Tilt	0.010	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.049	0.048	0.000	0.026	0.006
Twist	0.000	-0.035	-0.108	0.000	0.006	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.698	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	3.126	0.528	0.000	0.014	-0.144
Rise	0.000	0.528	6.856	0.000	-0.083	-0.222
Tilt	-0.098	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.014	-0.083	0.000	0.015	0.008
Twist	0.000	-0.144	-0.222	0.000	0.008	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.110	0.000	0.000	-0.145	0.000	0.000
Slide	0.000	3.312	0.926	0.000	0.009	-0.172
Rise	0.000	0.926	8.363	0.000	-0.064	-0.199
Tilt	-0.145	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.009	-0.064	0.000	0.016	0.005
Twist	0.000	-0.172	-0.199	0.000	0.005	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.052	0.000	0.000	0.007	0.000	0.000
Slide	0.000	1.428	0.060	0.000	-0.020	-0.096
Rise	0.000	0.060	0.510	0.000	0.003	-0.023
Tilt	0.007	0.000	0.000	0.010	0.000	0.000
Roll	0.000	-0.020	0.003	0.000	0.008	0.003
Twist	0.000	-0.096	-0.023	0.000	0.003	0.018

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.128	0.000	0.000	0.002	0.000	0.000
Slide	0.000	1.456	-0.420	0.000	-0.041	-0.095
Rise	0.000	-0.420	1.240	0.000	0.046	0.031
Tilt	0.002	0.000	0.000	0.007	0.000	0.000
Roll	0.000	-0.041	0.046	0.000	0.014	0.004
Twist	0.000	-0.095	0.031	0.000	0.004	0.013

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.457	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.520	1.164	0.000	0.004	-0.139
Rise	0.000	1.164	8.253	0.000	-0.091	-0.229
Tilt	-0.063	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.004	-0.091	0.000	0.016	0.010
Twist	0.000	-0.139	-0.229	0.000	0.010	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.481	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	2.068	1.100	0.000	0.011	-0.115
Rise	0.000	1.100	7.308	0.000	-0.076	-0.211
Tilt	-0.055	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.011	-0.076	0.000	0.015	0.009
Twist	0.000	-0.115	-0.211	0.000	0.009	0.029

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.10.cte	0.000	0.000

	Mean	Stdev
-acgt.5.cte	0.000	0.000

	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.11.cte	0.000	0.000

	Mean	Stdev
-cggg.6.cte	0.000	0.000

	Mean	Stdev
-gatg.7.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
rise-10-ACGT	3.394	0.280

	Mean	Stdev
roll-10-ACGT	-1.110	5.386

	Mean	Stdev
shift-10-ACGT	0.000	0.833

	Mean	Stdev
slide-10-ACGT	-0.559	0.453

	Mean	Stdev
tilt-10-ACGT	0.000	4.330

	Mean	Stdev
twist-10-ACGT	32.832	4.620

	Mean	Stdev
rise-11-CGCG	3.264	0.282

	Mean	Stdev
roll-11-CGCG	0.576	6.216

	Mean	Stdev
shift-11-CGCG	0.000	1.151

	Mean	Stdev
slide-11-CGCG	0.563	0.471

	Mean	Stdev
tilt-11-CGCG	0.000	6.814

	Mean	Stdev
twist-11-CGCG	37.551	4.107

	Mean	Stdev
rise-2-CATG	3.307	0.330

	Mean	Stdev
roll-2-CATG	7.954	6.952

	Mean	Stdev
shift-2-CATG	0.000	1.292

	Mean	Stdev
slide-2-CATG	-0.036	0.495

	Mean	Stdev
tilt-2-CATG	0.000	6.503

	Mean	Stdev
twist-2-CATG	34.104	5.245

	Mean	Stdev
rise-3-ATAT	3.274	0.256

	Mean	Stdev
roll-3-ATAT	-2.845	4.995

	Mean	Stdev
shift-3-ATAT	0.000	0.698

	Mean	Stdev
slide-3-ATAT	-0.724	0.399

	Mean	Stdev
tilt-3-ATAT	0.000	3.483

	Mean	Stdev
twist-3-ATAT	32.333	3.681

	Mean	Stdev
rise-4-TATA	3.223	0.298

	Mean	Stdev
roll-4-TATA	3.086	7.197

	Mean	Stdev
shift-4-TATA	0.000	1.383

	Mean	Stdev
slide-4-TATA	0.323	0.577

	Mean	Stdev
tilt-4-TATA	0.000	5.729

	Mean	Stdev
twist-4-TATA	37.791	5.801

	Mean	Stdev
rise-5-ACGT	3.209	0.285

	Mean	Stdev
roll-5-ACGT	-1.517	5.006

	Mean	Stdev
shift-5-ACGT	0.000	0.708

	Mean	Stdev
slide-5-ACGT	-0.525	0.448

	Mean	Stdev
tilt-5-ACGT	0.000	3.974

	Mean	Stdev
twist-5-ACGT	27.519	5.415

	Mean	Stdev
rise-6-CGGG	2.940	1.113

	Mean	Stdev
roll-6-CGGG	2.508	8.710

	Mean	Stdev
shift-6-CGGG	0.000	3.513

	Mean	Stdev
slide-6-CGGG	-1.341	0.802

	Mean	Stdev
tilt-6-CGGG	0.000	8.147

	Mean	Stdev
twist-6-CGGG	17.442	7.362

	Mean	Stdev
rise-7-GATG	4.062	0.758

	Mean	Stdev
roll-7-GATG	4.661	7.308

	Mean	Stdev
shift-7-GATG	0.000	2.144

	Mean	Stdev
slide-7-GATG	1.516	0.897

	Mean	Stdev
tilt-7-GATG	0.000	9.485

	Mean	Stdev
twist-7-GATG	56.437	9.466

	Mean	Stdev
rise-8-ATAT	3.228	0.271

	Mean	Stdev
roll-8-ATAT	-1.376	5.014

	Mean	Stdev
shift-8-ATAT	0.000	0.640

	Mean	Stdev
slide-8-ATAT	-0.772	0.400

	Mean	Stdev
tilt-8-ATAT	0.000	3.956

	Mean	Stdev
twist-8-ATAT	28.935	4.879

	Mean	Stdev
rise-9-TATA	3.318	0.320

	Mean	Stdev
roll-9-TATA	4.237	7.463

	Mean	Stdev
shift-9-TATA	0.000	1.290

	Mean	Stdev
slide-9-TATA	0.051	0.647

	Mean	Stdev
tilt-9-TATA	0.000	5.822

	Mean	Stdev
twist-9-TATA	37.085	6.322

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