NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	G	-	C	-	G	-	A	-	C	-	G	-	G	-	G	-	T	-	G	-	T	-	G	-	T	-	T	-	C	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.004	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.594	0.721	0.000	-0.070	-0.135
Rise	0.000	0.721	8.794	0.000	-0.011	-0.180
Tilt	-0.084	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.070	-0.011	0.000	0.025	0.011
Twist	0.000	-0.135	-0.180	0.000	0.011	0.031

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.842	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	3.651	3.277	0.000	0.026	-0.080
Rise	0.000	3.277	10.968	0.000	0.072	-0.224
Tilt	-0.036	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.026	0.072	0.000	0.025	0.009
Twist	0.000	-0.080	-0.224	0.000	0.009	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.679	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	4.101	3.163	0.000	0.044	-0.074
Rise	0.000	3.163	11.966	0.000	0.125	-0.200
Tilt	-0.029	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.044	0.125	0.000	0.027	0.011
Twist	0.000	-0.074	-0.200	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.547	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	2.717	1.610	0.000	-0.054	-0.143
Rise	0.000	1.610	7.541	0.000	-0.052	-0.144
Tilt	-0.110	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.054	-0.052	0.000	0.024	0.005
Twist	0.000	-0.143	-0.144	0.000	0.005	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.594	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.330	1.103	0.000	0.021	-0.088
Rise	0.000	1.103	7.615	0.000	-0.071	-0.205
Tilt	-0.082	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.021	-0.071	0.000	0.015	0.006
Twist	0.000	-0.088	-0.205	0.000	0.006	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.691	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	2.275	1.433	0.000	0.009	-0.110
Rise	0.000	1.433	7.356	0.000	-0.104	-0.244
Tilt	-0.066	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.009	-0.104	0.000	0.017	0.009
Twist	0.000	-0.110	-0.244	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.551	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	3.234	0.353	0.000	0.032	-0.133
Rise	0.000	0.353	8.740	0.000	-0.099	-0.240
Tilt	-0.081	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.032	-0.099	0.000	0.020	0.008
Twist	0.000	-0.133	-0.240	0.000	0.008	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.842	0.000	0.000	-0.116	0.000	0.000
Slide	0.000	2.102	1.914	0.000	-0.025	-0.090
Rise	0.000	1.914	7.941	0.000	-0.029	-0.179
Tilt	-0.116	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.025	-0.029	0.000	0.024	0.008
Twist	0.000	-0.090	-0.179	0.000	0.008	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.927	0.000	0.000	-0.134	0.000	0.000
Slide	0.000	2.250	1.830	0.000	-0.019	-0.101
Rise	0.000	1.830	9.300	0.000	-0.006	-0.174
Tilt	-0.134	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.019	-0.006	0.000	0.023	0.007
Twist	0.000	-0.101	-0.174	0.000	0.007	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.012	0.000	0.000	-0.115	0.000	0.000
Slide	0.000	2.782	1.671	0.000	-0.039	-0.136
Rise	0.000	1.671	9.743	0.000	-0.014	-0.205
Tilt	-0.115	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.039	-0.014	0.000	0.020	0.007
Twist	0.000	-0.136	-0.205	0.000	0.007	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.766	0.000	0.000	-0.146	0.000	0.000
Slide	0.000	2.419	2.469	0.000	0.047	-0.058
Rise	0.000	2.469	11.593	0.000	0.152	-0.226
Tilt	-0.146	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.047	0.152	0.000	0.030	0.007
Twist	0.000	-0.058	-0.227	0.000	0.007	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.114	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	3.564	3.216	0.000	0.056	-0.089
Rise	0.000	3.216	12.452	0.000	0.124	-0.230
Tilt	-0.086	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.056	0.124	0.000	0.025	0.005
Twist	0.000	-0.089	-0.230	0.000	0.005	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.766	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	3.186	0.613	0.000	0.014	-0.118
Rise	0.000	0.613	8.247	0.000	-0.141	-0.267
Tilt	-0.069	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.014	-0.141	0.000	0.022	0.012
Twist	0.000	-0.118	-0.267	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.9.cte	0.000	0.000

	Mean	Stdev
-agct.14.cte	0.000	0.000

	Mean	Stdev
-catg.10.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-catg.8.cte	0.000	0.000

	Mean	Stdev
-ccgg.6.cte	0.000	0.000

	Mean	Stdev
-ccgg.7.cte	0.000	0.000

	Mean	Stdev
-ctag.3.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gcgc.5.cte	0.000	0.000

	Mean	Stdev
-tgca.4.cte	0.000	0.000

	Mean	Stdev
rise-10-CATG	3.340	0.320

	Mean	Stdev
roll-10-CATG	5.240	6.800

	Mean	Stdev
shift-10-CATG	0.000	1.271

	Mean	Stdev
slide-10-CATG	-0.042	0.566

	Mean	Stdev
tilt-10-CATG	0.000	5.721

	Mean	Stdev
twist-10-CATG	36.878	6.354

	Mean	Stdev
rise-11-ACGT	3.295	0.291

	Mean	Stdev
roll-11-ACGT	-0.217	5.336

	Mean	Stdev
shift-11-ACGT	0.000	0.851

	Mean	Stdev
slide-11-ACGT	-0.560	0.469

	Mean	Stdev
tilt-11-ACGT	0.000	3.954

	Mean	Stdev
twist-11-ACGT	31.274	4.837

	Mean	Stdev
rise-12-CATG	3.286	0.356

	Mean	Stdev
roll-12-CATG	7.367	7.118

	Mean	Stdev
shift-12-CATG	0.000	1.051

	Mean	Stdev
slide-12-CATG	0.025	0.628

	Mean	Stdev
tilt-12-CATG	0.000	5.296

	Mean	Stdev
twist-12-CATG	34.222	8.067

	Mean	Stdev
rise-13-AATT	3.259	0.278

	Mean	Stdev
roll-13-AATT	0.905	5.367

	Mean	Stdev
shift-13-AATT	0.000	0.873

	Mean	Stdev
slide-13-AATT	-0.098	0.546

	Mean	Stdev
tilt-13-AATT	0.000	5.059

	Mean	Stdev
twist-13-AATT	35.068	5.590

	Mean	Stdev
rise-14-AGCT	3.474	0.301

	Mean	Stdev
roll-14-AGCT	2.473	5.060

	Mean	Stdev
shift-14-AGCT	0.000	0.702

	Mean	Stdev
slide-14-AGCT	-0.723	0.546

	Mean	Stdev
tilt-14-AGCT	0.000	4.748

	Mean	Stdev
twist-14-AGCT	35.644	4.513

	Mean	Stdev
rise-2-GCGC	3.512	0.280

	Mean	Stdev
roll-2-GCGC	-1.931	4.924

	Mean	Stdev
shift-2-GCGC	0.000	0.685

	Mean	Stdev
slide-2-GCGC	-0.522	0.559

	Mean	Stdev
tilt-2-GCGC	0.000	4.480

	Mean	Stdev
twist-2-GCGC	36.358	4.655

	Mean	Stdev
rise-3-CTAG	3.250	0.272

	Mean	Stdev
roll-3-CTAG	1.142	5.637

	Mean	Stdev
shift-3-CTAG	0.000	0.976

	Mean	Stdev
slide-3-CTAG	-0.184	0.533

	Mean	Stdev
tilt-3-CTAG	0.000	5.380

	Mean	Stdev
twist-3-CTAG	35.403	5.644

	Mean	Stdev
rise-4-TGCA	3.016	0.335

	Mean	Stdev
roll-4-TGCA	7.403	6.299

	Mean	Stdev
shift-4-TGCA	0.000	0.981

	Mean	Stdev
slide-4-TGCA	-0.084	0.508

	Mean	Stdev
tilt-4-TGCA	0.000	4.968

	Mean	Stdev
twist-4-TGCA	29.014	7.674

	Mean	Stdev
rise-5-GCGC	3.339	0.263

	Mean	Stdev
roll-5-GCGC	-0.228	5.148

	Mean	Stdev
shift-5-GCGC	0.000	0.793

	Mean	Stdev
slide-5-GCGC	-0.292	0.469

	Mean	Stdev
tilt-5-GCGC	0.000	4.141

	Mean	Stdev
twist-5-GCGC	35.336	4.399

	Mean	Stdev
rise-6-CCGG	3.575	0.317

	Mean	Stdev
roll-6-CCGG	3.702	5.209

	Mean	Stdev
shift-6-CCGG	0.000	0.621

	Mean	Stdev
slide-6-CCGG	-0.920	0.614

	Mean	Stdev
tilt-6-CCGG	0.000	4.147

	Mean	Stdev
twist-6-CCGG	36.252	5.061

	Mean	Stdev
rise-7-CCGG	3.350	0.279

	Mean	Stdev
roll-7-CCGG	3.719	5.237

	Mean	Stdev
shift-7-CCGG	0.000	1.052

	Mean	Stdev
slide-7-CCGG	-0.025	0.576

	Mean	Stdev
tilt-7-CCGG	0.000	4.752

	Mean	Stdev
twist-7-CCGG	35.100	4.266

	Mean	Stdev
rise-8-CATG	3.151	0.301

	Mean	Stdev
roll-8-CATG	5.146	6.069

	Mean	Stdev
shift-8-CATG	0.000	1.334

	Mean	Stdev
slide-8-CATG	0.131	0.506

	Mean	Stdev
tilt-8-CATG	0.000	5.732

	Mean	Stdev
twist-8-CATG	33.800	6.251

	Mean	Stdev
rise-9-ACGT	3.225	0.268

	Mean	Stdev
roll-9-ACGT	-0.922	5.332

	Mean	Stdev
shift-9-ACGT	0.000	0.943

	Mean	Stdev
slide-9-ACGT	-0.442	0.426

	Mean	Stdev
tilt-9-ACGT	0.000	3.843

	Mean	Stdev
twist-9-ACGT	31.165	4.619

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3'	G	-	C	-	G	-	A	-	C	-	G	-	G	-	G	-	T	-	G	-	T	-	G	-	T	-	T	-	C	-	G	5'
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