NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	C	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	C	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.976	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	4.201	3.329	0.000	0.004	-0.064
Rise	0.000	3.329	10.652	0.000	0.052	-0.245
Tilt	-0.055	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.004	0.052	0.000	0.027	0.012
Twist	0.000	-0.064	-0.245	0.000	0.012	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.163	0.000	0.000	-0.144	0.000	0.000
Slide	0.000	2.581	1.245	0.000	0.024	-0.082
Rise	0.000	1.245	7.333	0.000	-0.057	-0.228
Tilt	-0.144	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.024	-0.057	0.000	0.014	0.004
Twist	0.000	-0.082	-0.228	0.000	0.004	0.023

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.989	0.000	0.000	-0.109	0.000	0.000
Slide	0.000	3.005	0.325	0.000	-0.001	-0.133
Rise	0.000	0.326	8.648	0.000	-0.139	-0.296
Tilt	-0.109	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.001	-0.139	0.000	0.019	0.009
Twist	0.000	-0.133	-0.296	0.000	0.009	0.026

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.229	0.000	0.000	-0.147	0.000	0.000
Slide	0.000	2.623	1.224	0.000	0.019	-0.090
Rise	0.000	1.224	7.468	0.000	-0.072	-0.249
Tilt	-0.147	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.019	-0.072	0.000	0.015	0.003
Twist	0.000	-0.090	-0.249	0.000	0.003	0.022

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.859	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	2.381	1.438	0.000	-0.049	-0.126
Rise	0.000	1.438	8.739	0.000	-0.046	-0.184
Tilt	-0.107	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.049	-0.046	0.000	0.022	0.011
Twist	0.000	-0.126	-0.184	0.000	0.011	0.037

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.318	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	2.574	2.164	0.000	0.047	-0.044
Rise	0.000	2.164	11.096	0.000	0.134	-0.207
Tilt	-0.112	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.047	0.134	0.000	0.027	0.008
Twist	0.000	-0.044	-0.207	0.000	0.008	0.033

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.313	0.000	0.000	-0.138	0.000	0.000
Slide	0.000	2.543	2.160	0.000	0.060	-0.048
Rise	0.000	2.160	10.432	0.000	0.123	-0.188
Tilt	-0.138	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.060	0.123	0.000	0.027	0.009
Twist	0.000	-0.048	-0.188	0.000	0.009	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.048	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	3.868	3.244	0.000	-0.001	-0.054
Rise	0.000	3.244	10.421	0.000	0.047	-0.229
Tilt	-0.050	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.001	0.047	0.000	0.026	0.009
Twist	0.000	-0.054	-0.229	0.000	0.009	0.034

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.864	0.000	0.000	-0.092	0.000	0.000
Slide	0.000	2.135	1.480	0.000	-0.042	-0.120
Rise	0.000	1.480	8.498	0.000	-0.042	-0.165
Tilt	-0.092	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.042	-0.042	0.000	0.022	0.010
Twist	0.000	-0.120	-0.165	0.000	0.010	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-acgt.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.6.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gtac.4.cte	0.000	0.000

	Mean	Stdev
-tcga.5.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.368	0.279

	Mean	Stdev
roll-10-GCGC	-0.989	5.256

	Mean	Stdev
shift-10-GCGC	0.000	0.769

	Mean	Stdev
slide-10-GCGC	-0.365	0.525

	Mean	Stdev
tilt-10-GCGC	0.000	4.477

	Mean	Stdev
twist-10-GCGC	33.830	4.906

	Mean	Stdev
rise-2-GCGC	3.393	0.285

	Mean	Stdev
roll-2-GCGC	-1.381	5.328

	Mean	Stdev
shift-2-GCGC	0.000	0.826

	Mean	Stdev
slide-2-GCGC	-0.316	0.538

	Mean	Stdev
tilt-2-GCGC	0.000	4.623

	Mean	Stdev
twist-2-GCGC	33.790	5.097

	Mean	Stdev
rise-3-CGCG	3.332	0.344

	Mean	Stdev
roll-3-CGCG	6.272	6.697

	Mean	Stdev
shift-3-CGCG	0.000	1.044

	Mean	Stdev
slide-3-CGCG	0.090	0.524

	Mean	Stdev
tilt-3-CGCG	0.000	6.183

	Mean	Stdev
twist-3-CGCG	37.366	6.358

	Mean	Stdev
rise-4-GTAC	3.352	0.306

	Mean	Stdev
roll-4-GTAC	-0.459	5.142

	Mean	Stdev
shift-4-GTAC	0.000	0.772

	Mean	Stdev
slide-4-GTAC	-0.674	0.455

	Mean	Stdev
tilt-4-GTAC	0.000	4.000

	Mean	Stdev
twist-4-GTAC	31.340	4.910

	Mean	Stdev
rise-5-TCGA	3.359	0.285

	Mean	Stdev
roll-5-TCGA	0.897	5.534

	Mean	Stdev
shift-5-TCGA	0.000	0.995

	Mean	Stdev
slide-5-TCGA	0.069	0.604

	Mean	Stdev
tilt-5-TCGA	0.000	5.256

	Mean	Stdev
twist-5-TCGA	38.189	4.888

	Mean	Stdev
rise-6-CGCG	3.060	0.357

	Mean	Stdev
roll-6-CGCG	8.011	6.298

	Mean	Stdev
shift-6-CGCG	0.000	0.976

	Mean	Stdev
slide-6-CGCG	0.107	0.549

	Mean	Stdev
tilt-6-CGCG	0.000	5.415

	Mean	Stdev
twist-6-CGCG	28.783	7.891

	Mean	Stdev
rise-7-GATC	3.355	0.280

	Mean	Stdev
roll-7-GATC	1.277	5.508

	Mean	Stdev
shift-7-GATC	0.000	1.066

	Mean	Stdev
slide-7-GATC	0.150	0.561

	Mean	Stdev
tilt-7-GATC	0.000	5.386

	Mean	Stdev
twist-7-GATC	38.228	4.724

	Mean	Stdev
rise-8-ACGT	3.321	0.301

	Mean	Stdev
roll-8-ACGT	-0.067	5.287

	Mean	Stdev
shift-8-ACGT	0.000	0.808

	Mean	Stdev
slide-8-ACGT	-0.612	0.432

	Mean	Stdev
tilt-8-ACGT	0.000	4.046

	Mean	Stdev
twist-8-ACGT	31.328	4.712

	Mean	Stdev
rise-9-CGCG	3.359	0.361

	Mean	Stdev
roll-9-CGCG	6.729	6.533

	Mean	Stdev
shift-9-CGCG	0.000	0.990

	Mean	Stdev
slide-9-CGCG	0.094	0.521

	Mean	Stdev
tilt-9-CGCG	0.000	5.930

	Mean	Stdev
twist-9-CGCG	37.835	6.958

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	C	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	C	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	C	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	C	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	C	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	C	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13