NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	X	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	X	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.075	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	3.869	3.152	0.000	0.013	-0.063
Rise	0.000	3.152	10.567	0.000	0.070	-0.232
Tilt	-0.052	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.013	0.070	0.000	0.027	0.010
Twist	0.000	-0.063	-0.232	0.000	0.010	0.042

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.192	0.000	0.000	-0.153	0.000	0.000
Slide	0.000	2.602	1.351	0.000	0.024	-0.084
Rise	0.000	1.351	7.750	0.000	-0.059	-0.217
Tilt	-0.153	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.024	-0.059	0.000	0.014	0.003
Twist	0.000	-0.084	-0.217	0.000	0.003	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.182	0.000	0.000	-0.146	0.000	0.000
Slide	0.000	2.601	1.515	0.000	0.019	-0.099
Rise	0.000	1.515	7.587	0.000	-0.053	-0.202
Tilt	-0.146	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.019	-0.053	0.000	0.014	0.002
Twist	0.000	-0.099	-0.202	0.000	0.002	0.023

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.000	0.000	0.000	0.000	0.000	0.000
Slide	0.000	0.000	0.000	0.000	0.000	0.000
Rise	0.000	0.000	0.000	0.000	0.000	0.000
Tilt	0.000	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.000	0.000	0.000	0.000	0.000
Twist	0.000	0.000	0.000	0.000	0.000	0.000

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.284	0.000	0.000	-0.136	0.000	0.000
Slide	0.000	2.781	2.067	0.000	0.061	-0.041
Rise	0.000	2.067	10.420	0.000	0.115	-0.187
Tilt	-0.136	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.061	0.115	0.000	0.026	0.010
Twist	0.000	-0.041	-0.187	0.000	0.010	0.030

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.331	0.000	0.000	-0.146	0.000	0.000
Slide	0.000	2.757	2.392	0.000	0.071	-0.044
Rise	0.000	2.392	11.195	0.000	0.141	-0.214
Tilt	-0.146	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.071	0.141	0.000	0.027	0.008
Twist	0.000	-0.044	-0.214	0.000	0.008	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.905	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	3.758	3.194	0.000	0.004	-0.052
Rise	0.000	3.194	10.470	0.000	0.049	-0.229
Tilt	-0.054	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.004	0.049	0.000	0.028	0.012
Twist	0.000	-0.052	-0.229	0.000	0.012	0.043

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.000	0.000	0.000	0.000	0.000	0.000
Slide	0.000	0.000	0.000	0.000	0.000	0.000
Rise	0.000	0.000	0.000	0.000	0.000	0.000
Tilt	0.000	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.000	0.000	0.000	0.000	0.000
Twist	0.000	0.000	0.000	0.000	0.000	0.000

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.000	0.000	0.000	0.000	0.000	0.000
Slide	0.000	0.000	0.000	0.000	0.000	0.000
Rise	0.000	0.000	0.000	0.000	0.000	0.000
Tilt	0.000	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.000	0.000	0.000	0.000	0.000
Twist	0.000	0.000	0.000	0.000	0.000	0.000

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-acgt.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatx.7.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gtac.4.cte	0.000	0.000

	Mean	Stdev
-txga.5.cte	0.000	0.000

	Mean	Stdev
-xgxg.6.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.374	0.284

	Mean	Stdev
roll-10-GCGC	-1.211	5.552

	Mean	Stdev
shift-10-GCGC	0.000	0.834

	Mean	Stdev
slide-10-GCGC	-0.259	0.506

	Mean	Stdev
tilt-10-GCGC	0.000	4.617

	Mean	Stdev
twist-10-GCGC	32.897	5.349

	Mean	Stdev
rise-2-GCGC	3.381	0.282

	Mean	Stdev
roll-2-GCGC	-1.681	5.346

	Mean	Stdev
shift-2-GCGC	0.000	0.837

	Mean	Stdev
slide-2-GCGC	-0.307	0.520

	Mean	Stdev
tilt-2-GCGC	0.000	4.641

	Mean	Stdev
twist-2-GCGC	33.435	5.041

	Mean	Stdev
rise-3-CGCG	3.383	0.326

	Mean	Stdev
roll-3-CGCG	5.592	6.672

	Mean	Stdev
shift-3-CGCG	0.000	1.077

	Mean	Stdev
slide-3-CGCG	0.093	0.522

	Mean	Stdev
tilt-3-CGCG	0.000	6.352

	Mean	Stdev
twist-3-CGCG	38.078	5.806

	Mean	Stdev
rise-4-GTAC	3.371	0.299

	Mean	Stdev
roll-4-GTAC	-0.855	5.037

	Mean	Stdev
shift-4-GTAC	0.000	0.839

	Mean	Stdev
slide-4-GTAC	-0.671	0.461

	Mean	Stdev
tilt-4-GTAC	0.000	3.980

	Mean	Stdev
twist-4-GTAC	33.003	4.276

	Mean	Stdev
rise-5-TXGA	0.000	0.000

	Mean	Stdev
roll-5-TXGA	0.000	0.000

	Mean	Stdev
shift-5-TXGA	0.000	0.000

	Mean	Stdev
slide-5-TXGA	0.000	0.000

	Mean	Stdev
tilt-5-TXGA	0.000	0.000

	Mean	Stdev
twist-5-TXGA	0.000	0.000

	Mean	Stdev
rise-6-XGXG	0.000	0.000

	Mean	Stdev
roll-6-XGXG	0.000	0.000

	Mean	Stdev
shift-6-XGXG	0.000	0.000

	Mean	Stdev
slide-6-XGXG	0.000	0.000

	Mean	Stdev
tilt-6-XGXG	0.000	0.000

	Mean	Stdev
twist-6-XGXG	0.000	0.000

	Mean	Stdev
rise-7-GATX	0.000	0.000

	Mean	Stdev
roll-7-GATX	0.000	0.000

	Mean	Stdev
shift-7-GATX	0.000	0.000

	Mean	Stdev
slide-7-GATX	0.000	0.000

	Mean	Stdev
tilt-7-GATX	0.000	0.000

	Mean	Stdev
twist-7-GATX	0.000	0.000

	Mean	Stdev
rise-8-ACGT	3.408	0.295

	Mean	Stdev
roll-8-ACGT	-1.343	5.072

	Mean	Stdev
shift-8-ACGT	0.000	0.763

	Mean	Stdev
slide-8-ACGT	-0.726	0.448

	Mean	Stdev
tilt-8-ACGT	0.000	3.943

	Mean	Stdev
twist-8-ACGT	33.506	4.338

	Mean	Stdev
rise-9-CGCG	3.398	0.328

	Mean	Stdev
roll-9-CGCG	5.324	6.587

	Mean	Stdev
shift-9-CGCG	0.000	1.035

	Mean	Stdev
slide-9-CGCG	0.074	0.537

	Mean	Stdev
tilt-9-CGCG	0.000	6.134

	Mean	Stdev
twist-9-CGCG	38.122	6.084

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	X	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	X	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	X	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	X	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13

	1		2		3		4		5		6		7		8		9		10		11		12
5'	C	-	G	-	C	-	G	-	T	-	X	-	G	-	A	-	C	-	G	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	G	-	C	-	A	-	G	-	X	-	T	-	G	-	C	-	G	-	C	5'
	24		23		22		21		20		19		18		17		16		15		14		13