NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.971	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	3.542	1.235	0.000	-0.057	-0.268
Rise	0.000	1.235	7.393	0.000	-0.071	-0.159
Tilt	-0.044	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.057	-0.071	0.000	0.021	0.008
Twist	0.000	-0.268	-0.159	0.000	0.008	0.048

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.228	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.492	0.827	0.000	-0.081	-0.159
Rise	0.000	0.826	7.563	0.000	-0.015	-0.176
Tilt	-0.082	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.081	-0.015	0.000	0.022	0.011
Twist	0.000	-0.159	-0.176	0.000	0.011	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.044	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	3.856	1.196	0.000	-0.053	-0.289
Rise	0.000	1.196	7.650	0.000	-0.058	-0.155
Tilt	-0.059	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.053	-0.058	0.000	0.023	0.007
Twist	0.000	-0.289	-0.155	0.000	0.007	0.052

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.302	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	2.520	0.879	0.000	-0.084	-0.159
Rise	0.000	0.879	7.354	0.000	-0.032	-0.172
Tilt	-0.079	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.084	-0.032	0.000	0.023	0.012
Twist	0.000	-0.159	-0.172	0.000	0.012	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.976	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	3.768	1.248	0.000	-0.063	-0.295
Rise	0.000	1.248	7.350	0.000	-0.062	-0.154
Tilt	-0.058	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.063	-0.062	0.000	0.021	0.008
Twist	0.000	-0.295	-0.154	0.000	0.008	0.051

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.174	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	2.320	0.813	0.000	-0.078	-0.150
Rise	0.000	0.813	7.640	0.000	-0.018	-0.177
Tilt	-0.074	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.078	-0.018	0.000	0.022	0.011
Twist	0.000	-0.150	-0.177	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.166	0.000	0.000	0.005	0.000	0.000
Slide	0.000	4.047	2.478	0.000	-0.011	-0.142
Rise	0.000	2.478	11.081	0.000	0.105	-0.151
Tilt	0.005	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.011	0.105	0.000	0.025	0.011
Twist	0.000	-0.142	-0.151	0.000	0.011	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.482	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	4.195	2.228	0.000	0.005	-0.166
Rise	0.000	2.228	9.884	0.000	0.063	-0.141
Tilt	-0.011	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.005	0.063	0.000	0.028	0.009
Twist	0.000	-0.166	-0.141	0.000	0.009	0.055

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.019	0.000	0.000	0.019	0.000	0.000
Slide	0.000	3.565	2.195	0.000	-0.028	-0.040
Rise	0.000	2.195	10.150	0.000	0.062	-0.140
Tilt	0.019	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.028	0.062	0.000	0.026	0.012
Twist	0.000	-0.040	-0.140	0.000	0.012	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.201	0.000	0.000	0.007	0.000	0.000
Slide	0.000	4.055	2.438	0.000	-0.015	-0.170
Rise	0.000	2.438	10.701	0.000	0.078	-0.170
Tilt	0.007	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.015	0.078	0.000	0.026	0.012
Twist	0.000	-0.170	-0.170	0.000	0.012	0.051

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.249	0.000	0.000	0.015	0.000	0.000
Slide	0.000	0.647	-0.011	0.000	-0.005	0.010
Rise	0.000	-0.011	2.323	0.000	-0.035	-0.035
Tilt	0.015	0.000	0.000	0.010	0.000	0.000
Roll	0.000	-0.005	-0.035	0.000	0.004	-0.000
Twist	0.000	0.010	-0.035	0.000	-0.000	0.005

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.858	0.000	0.000	-0.013	0.000	0.000
Slide	0.000	1.334	1.021	0.000	0.002	-0.037
Rise	0.000	1.021	6.429	0.000	-0.095	-0.195
Tilt	-0.013	0.000	0.000	0.021	0.000	0.000
Roll	0.000	0.002	-0.095	0.000	0.018	0.011
Twist	0.000	-0.037	-0.195	0.000	0.011	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.785	0.000	0.000	-0.014	0.000	0.000
Slide	0.000	1.360	0.986	0.000	0.003	-0.051
Rise	0.000	0.986	6.116	0.000	-0.092	-0.193
Tilt	-0.014	0.000	0.000	0.020	0.000	0.000
Roll	0.000	0.003	-0.092	0.000	0.017	0.011
Twist	0.000	-0.051	-0.193	0.000	0.011	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.833	0.000	0.000	-0.014	0.000	0.000
Slide	0.000	1.290	0.942	0.000	0.006	-0.031
Rise	0.000	0.942	6.297	0.000	-0.090	-0.183
Tilt	-0.014	0.000	0.000	0.021	0.000	0.000
Roll	0.000	0.006	-0.090	0.000	0.017	0.011
Twist	0.000	-0.031	-0.183	0.000	0.011	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.354	0.000	0.000	0.023	0.000	0.000
Slide	0.000	0.764	-0.009	0.000	-0.007	0.019
Rise	0.000	-0.009	2.637	0.000	-0.080	-0.085
Tilt	0.023	0.000	0.000	0.013	0.000	0.000
Roll	0.000	-0.007	-0.080	0.000	0.007	0.000
Twist	0.000	0.019	-0.085	0.000	0.000	0.006

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-atat.11.cte	0.000	0.000

	Mean	Stdev
-atat.15.cte	0.000	0.000

	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-tata.12.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.8.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.333	0.295

	Mean	Stdev
roll-10-AATT	0.051	5.452

	Mean	Stdev
shift-10-AATT	0.000	0.556

	Mean	Stdev
slide-10-AATT	-0.611	0.537

	Mean	Stdev
tilt-10-AATT	0.000	4.918

	Mean	Stdev
twist-10-AATT	35.543	4.663

	Mean	Stdev
rise-11-ATAT	3.246	0.259

	Mean	Stdev
roll-11-ATAT	-1.734	5.341

	Mean	Stdev
shift-11-ATAT	0.000	0.709

	Mean	Stdev
slide-11-ATAT	-0.849	0.426

	Mean	Stdev
tilt-11-ATAT	0.000	3.836

	Mean	Stdev
twist-11-ATAT	31.177	4.039

	Mean	Stdev
rise-12-TATA	3.290	0.373

	Mean	Stdev
roll-12-TATA	10.558	7.119

	Mean	Stdev
shift-12-TATA	0.000	0.831

	Mean	Stdev
slide-12-TATA	-0.381	0.719

	Mean	Stdev
tilt-12-TATA	0.000	5.305

	Mean	Stdev
twist-12-TATA	29.747	7.650

	Mean	Stdev
rise-13-AATT	3.327	0.301

	Mean	Stdev
roll-13-AATT	0.729	5.745

	Mean	Stdev
shift-13-AATT	0.000	0.757

	Mean	Stdev
slide-13-AATT	-0.212	0.595

	Mean	Stdev
tilt-13-AATT	0.000	4.596

	Mean	Stdev
twist-13-AATT	35.110	5.847

	Mean	Stdev
rise-14-AATT	3.379	0.288

	Mean	Stdev
roll-14-AATT	0.514	5.179

	Mean	Stdev
shift-14-AATT	0.000	0.553

	Mean	Stdev
slide-14-AATT	-0.524	0.512

	Mean	Stdev
tilt-14-AATT	0.000	4.747

	Mean	Stdev
twist-14-AATT	36.309	4.439

	Mean	Stdev
rise-15-ATAT	3.319	0.263

	Mean	Stdev
roll-15-ATAT	-1.887	4.748

	Mean	Stdev
shift-15-ATAT	0.000	0.630

	Mean	Stdev
slide-15-ATAT	-0.808	0.419

	Mean	Stdev
tilt-15-ATAT	0.000	3.686

	Mean	Stdev
twist-15-ATAT	31.559	3.609

	Mean	Stdev
rise-16-TGCA	3.219	0.925

	Mean	Stdev
roll-16-TGCA	6.031	13.089

	Mean	Stdev
shift-16-TGCA	0.000	1.372

	Mean	Stdev
slide-16-TGCA	-0.169	0.942

	Mean	Stdev
tilt-16-TGCA	0.000	7.158

	Mean	Stdev
twist-16-TGCA	29.833	15.795

	Mean	Stdev
rise-2-CATG	3.227	0.573

	Mean	Stdev
roll-2-CATG	4.185	12.720

	Mean	Stdev
shift-2-CATG	0.000	1.605

	Mean	Stdev
slide-2-CATG	-0.106	0.970

	Mean	Stdev
tilt-2-CATG	0.000	8.002

	Mean	Stdev
twist-2-CATG	30.807	11.526

	Mean	Stdev
rise-3-ATAT	3.190	0.276

	Mean	Stdev
roll-3-ATAT	0.085	5.511

	Mean	Stdev
shift-3-ATAT	0.000	0.762

	Mean	Stdev
slide-3-ATAT	-0.698	0.441

	Mean	Stdev
tilt-3-ATAT	0.000	4.040

	Mean	Stdev
twist-3-ATAT	29.094	4.984

	Mean	Stdev
rise-4-TATA	3.358	0.376

	Mean	Stdev
roll-4-TATA	10.441	7.480

	Mean	Stdev
shift-4-TATA	0.000	0.870

	Mean	Stdev
slide-4-TATA	-0.345	0.729

	Mean	Stdev
tilt-4-TATA	0.000	5.451

	Mean	Stdev
twist-4-TATA	31.360	7.564

	Mean	Stdev
rise-5-AATT	3.329	0.304

	Mean	Stdev
roll-5-AATT	1.425	5.716

	Mean	Stdev
shift-5-AATT	0.000	0.729

	Mean	Stdev
slide-5-AATT	-0.243	0.593

	Mean	Stdev
tilt-5-AATT	0.000	4.726

	Mean	Stdev
twist-5-AATT	34.844	5.921

	Mean	Stdev
rise-6-AATT	3.348	0.294

	Mean	Stdev
roll-6-AATT	0.295	5.525

	Mean	Stdev
shift-6-AATT	0.000	0.565

	Mean	Stdev
slide-6-AATT	-0.585	0.536

	Mean	Stdev
tilt-6-AATT	0.000	5.051

	Mean	Stdev
twist-6-AATT	35.772	4.642

	Mean	Stdev
rise-7-ATAT	3.250	0.260

	Mean	Stdev
roll-7-ATAT	-1.841	5.215

	Mean	Stdev
shift-7-ATAT	0.000	0.699

	Mean	Stdev
slide-7-ATAT	-0.839	0.432

	Mean	Stdev
tilt-7-ATAT	0.000	3.814

	Mean	Stdev
twist-7-ATAT	31.342	3.988

	Mean	Stdev
rise-8-TATA	3.277	0.372

	Mean	Stdev
roll-8-TATA	10.421	7.383

	Mean	Stdev
shift-8-TATA	0.000	0.844

	Mean	Stdev
slide-8-TATA	-0.365	0.728

	Mean	Stdev
tilt-8-TATA	0.000	5.262

	Mean	Stdev
twist-8-TATA	29.695	7.708

	Mean	Stdev
rise-9-AATT	3.311	0.301

	Mean	Stdev
roll-9-AATT	0.788	5.921

	Mean	Stdev
shift-9-AATT	0.000	0.767

	Mean	Stdev
slide-9-AATT	-0.241	0.615

	Mean	Stdev
tilt-9-AATT	0.000	4.716

	Mean	Stdev
twist-9-AATT	34.953	5.978

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