NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.995	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	3.620	0.899	0.000	-0.048	-0.275
Rise	0.000	0.899	8.422	0.000	0.010	-0.174
Tilt	-0.100	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.048	0.010	0.000	0.022	0.009
Twist	0.000	-0.275	-0.174	0.000	0.009	0.051

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.063	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	3.400	1.118	0.000	-0.058	-0.275
Rise	0.000	1.118	8.251	0.000	-0.013	-0.183
Tilt	-0.094	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.058	-0.013	0.000	0.021	0.011
Twist	0.000	-0.275	-0.183	0.000	0.011	0.050

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.951	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	3.686	1.013	0.000	-0.033	-0.285
Rise	0.000	1.013	8.040	0.000	0.012	-0.164
Tilt	-0.098	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.033	0.012	0.000	0.020	0.008
Twist	0.000	-0.285	-0.164	0.000	0.008	0.053

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.043	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	5.139	2.403	0.000	-0.047	-0.200
Rise	0.000	2.403	9.129	0.000	0.020	-0.158
Tilt	-0.001	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.047	0.020	0.000	0.033	0.009
Twist	0.000	-0.200	-0.158	0.000	0.009	0.061

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.474	0.000	0.000	-0.017	0.000	0.000
Slide	0.000	4.669	2.543	0.000	-0.017	-0.206
Rise	0.000	2.543	9.387	0.000	0.044	-0.193
Tilt	-0.017	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.017	0.044	0.000	0.030	0.011
Twist	0.000	-0.206	-0.193	0.000	0.011	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.562	0.000	0.000	-0.003	0.000	0.000
Slide	0.000	4.680	2.780	0.000	-0.057	-0.135
Rise	0.000	2.780	9.532	0.000	0.042	-0.166
Tilt	-0.003	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.057	0.042	0.000	0.032	0.013
Twist	0.000	-0.135	-0.166	0.000	0.013	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.006	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	5.244	2.105	0.000	-0.050	-0.213
Rise	0.000	2.105	9.054	0.000	0.011	-0.162
Tilt	-0.011	0.000	0.000	0.047	0.000	0.000
Roll	0.000	-0.050	0.011	0.000	0.034	0.011
Twist	0.000	-0.213	-0.162	0.000	0.011	0.064

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.498	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	2.049	0.863	0.000	0.010	-0.021
Rise	0.000	0.863	7.195	0.000	-0.171	-0.237
Tilt	-0.027	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.010	-0.171	0.000	0.025	0.010
Twist	0.000	-0.021	-0.237	0.000	0.010	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.418	0.000	0.000	0.012	0.000	0.000
Slide	0.000	1.442	0.616	0.000	-0.005	-0.051
Rise	0.000	0.616	3.711	0.000	-0.050	-0.094
Tilt	0.012	0.000	0.000	0.015	0.000	0.000
Roll	0.000	-0.005	-0.050	0.000	0.006	0.000
Twist	0.000	-0.051	-0.094	0.000	0.000	0.015

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.639	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	1.924	0.749	0.000	0.006	-0.016
Rise	0.000	0.749	7.100	0.000	-0.162	-0.229
Tilt	-0.031	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.006	-0.162	0.000	0.025	0.010
Twist	0.000	-0.016	-0.229	0.000	0.010	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.651	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	2.068	0.916	0.000	-0.000	-0.029
Rise	0.000	0.916	7.526	0.000	-0.169	-0.247
Tilt	-0.031	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.000	-0.169	0.000	0.026	0.011
Twist	0.000	-0.029	-0.247	0.000	0.011	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.986	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.341	1.149	0.000	-0.037	-0.191
Rise	0.000	1.149	7.748	0.000	-0.032	-0.148
Tilt	-0.084	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.037	-0.032	0.000	0.022	0.011
Twist	0.000	-0.191	-0.148	0.000	0.011	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.086	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.443	1.352	0.000	-0.043	-0.218
Rise	0.000	1.352	7.705	0.000	-0.041	-0.173
Tilt	-0.088	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.043	-0.041	0.000	0.021	0.011
Twist	0.000	-0.218	-0.173	0.000	0.011	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.993	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.643	1.093	0.000	-0.041	-0.226
Rise	0.000	1.093	7.872	0.000	-0.029	-0.139
Tilt	-0.084	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.041	-0.029	0.000	0.022	0.011
Twist	0.000	-0.226	-0.139	0.000	0.011	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.614	0.000	0.000	-0.004	0.000	0.000
Slide	0.000	1.343	0.720	0.000	0.004	-0.054
Rise	0.000	0.720	3.831	0.000	-0.044	-0.124
Tilt	-0.004	0.000	0.000	0.018	0.000	0.000
Roll	0.000	0.004	-0.044	0.000	0.009	0.001
Twist	0.000	-0.054	-0.124	0.000	0.001	0.012

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-atat.11.cte	0.000	0.000

	Mean	Stdev
-atat.15.cte	0.000	0.000

	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-catg.13.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.5.cte	0.000	0.000

	Mean	Stdev
-catg.9.cte	0.000	0.000

	Mean	Stdev
-tcga.12.cte	0.000	0.000

	Mean	Stdev
-tcga.4.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.365	0.275

	Mean	Stdev
roll-10-AATT	-0.834	5.408

	Mean	Stdev
shift-10-AATT	0.000	0.943

	Mean	Stdev
slide-10-AATT	-0.026	0.524

	Mean	Stdev
tilt-10-AATT	0.000	5.437

	Mean	Stdev
twist-10-AATT	38.337	4.654

	Mean	Stdev
rise-11-ATAT	3.390	0.273

	Mean	Stdev
roll-11-ATAT	-1.816	4.290

	Mean	Stdev
shift-11-ATAT	0.000	0.536

	Mean	Stdev
slide-11-ATAT	-0.835	0.380

	Mean	Stdev
tilt-11-ATAT	0.000	3.562

	Mean	Stdev
twist-11-ATAT	32.170	3.371

	Mean	Stdev
rise-12-TCGA	3.452	0.288

	Mean	Stdev
roll-12-TCGA	1.356	5.513

	Mean	Stdev
shift-12-TCGA	0.000	0.875

	Mean	Stdev
slide-12-TCGA	-0.001	0.640

	Mean	Stdev
tilt-12-TCGA	0.000	4.836

	Mean	Stdev
twist-12-TCGA	38.835	5.015

	Mean	Stdev
rise-13-CATG	3.055	0.387

	Mean	Stdev
roll-13-CATG	12.428	5.593

	Mean	Stdev
shift-13-CATG	0.000	0.631

	Mean	Stdev
slide-13-CATG	-0.117	0.555

	Mean	Stdev
tilt-13-CATG	0.000	4.557

	Mean	Stdev
twist-13-CATG	22.481	7.592

	Mean	Stdev
rise-14-AATT	3.324	0.279

	Mean	Stdev
roll-14-AATT	-0.302	5.623

	Mean	Stdev
shift-14-AATT	0.000	0.885

	Mean	Stdev
slide-14-AATT	-0.073	0.557

	Mean	Stdev
tilt-14-AATT	0.000	5.451

	Mean	Stdev
twist-14-AATT	37.692	4.868

	Mean	Stdev
rise-15-ATAT	3.369	0.276

	Mean	Stdev
roll-15-ATAT	-1.201	4.629

	Mean	Stdev
shift-15-ATAT	0.000	0.632

	Mean	Stdev
slide-15-ATAT	-0.817	0.408

	Mean	Stdev
tilt-15-ATAT	0.000	3.811

	Mean	Stdev
twist-15-ATAT	31.848	3.695

	Mean	Stdev
rise-16-TGCA	3.493	0.497

	Mean	Stdev
roll-16-TGCA	8.835	8.305

	Mean	Stdev
shift-16-TGCA	0.000	0.978

	Mean	Stdev
slide-16-TGCA	-0.349	0.737

	Mean	Stdev
tilt-16-TGCA	0.000	5.700

	Mean	Stdev
twist-16-TGCA	35.078	8.964

	Mean	Stdev
rise-2-CATG	3.441	0.465

	Mean	Stdev
roll-2-CATG	7.605	10.195

	Mean	Stdev
shift-2-CATG	0.000	1.200

	Mean	Stdev
slide-2-CATG	-0.375	0.688

	Mean	Stdev
tilt-2-CATG	0.000	6.424

	Mean	Stdev
twist-2-CATG	34.906	7.105

	Mean	Stdev
rise-3-ATAT	3.316	0.282

	Mean	Stdev
roll-3-ATAT	-0.924	4.689

	Mean	Stdev
shift-3-ATAT	0.000	0.613

	Mean	Stdev
slide-3-ATAT	-0.779	0.402

	Mean	Stdev
tilt-3-ATAT	0.000	3.814

	Mean	Stdev
twist-3-ATAT	30.680	3.948

	Mean	Stdev
rise-4-TCGA	3.395	0.293

	Mean	Stdev
roll-4-TCGA	1.417	5.647

	Mean	Stdev
shift-4-TCGA	0.000	0.839

	Mean	Stdev
slide-4-TCGA	0.015	0.657

	Mean	Stdev
tilt-4-TCGA	0.000	5.028

	Mean	Stdev
twist-4-TCGA	38.555	5.007

	Mean	Stdev
rise-5-CATG	3.055	0.379

	Mean	Stdev
roll-5-CATG	12.403	5.565

	Mean	Stdev
shift-5-CATG	0.000	0.605

	Mean	Stdev
slide-5-CATG	-0.123	0.568

	Mean	Stdev
tilt-5-CATG	0.000	4.638

	Mean	Stdev
twist-5-CATG	22.287	7.412

	Mean	Stdev
rise-6-AATT	3.385	0.281

	Mean	Stdev
roll-6-AATT	-0.666	5.600

	Mean	Stdev
shift-6-AATT	0.000	0.963

	Mean	Stdev
slide-6-AATT	-0.020	0.524

	Mean	Stdev
tilt-6-AATT	0.000	5.393

	Mean	Stdev
twist-6-AATT	38.515	4.587

	Mean	Stdev
rise-7-ATAT	3.398	0.270

	Mean	Stdev
roll-7-ATAT	-1.587	4.295

	Mean	Stdev
shift-7-ATAT	0.000	0.541

	Mean	Stdev
slide-7-ATAT	-0.831	0.372

	Mean	Stdev
tilt-7-ATAT	0.000	3.535

	Mean	Stdev
twist-7-ATAT	32.138	3.365

	Mean	Stdev
rise-8-TCGA	3.449	0.282

	Mean	Stdev
roll-8-TCGA	1.265	5.409

	Mean	Stdev
shift-8-TCGA	0.000	0.868

	Mean	Stdev
slide-8-TCGA	0.011	0.619

	Mean	Stdev
tilt-8-TCGA	0.000	4.756

	Mean	Stdev
twist-8-TCGA	38.866	4.802

	Mean	Stdev
rise-9-CATG	3.038	0.370

	Mean	Stdev
roll-9-CATG	12.659	5.458

	Mean	Stdev
shift-9-CATG	0.000	0.602

	Mean	Stdev
slide-9-CATG	-0.120	0.550

	Mean	Stdev
tilt-9-CATG	0.000	4.588

	Mean	Stdev
twist-9-CATG	21.985	7.231

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