NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.982	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	1.983	1.516	0.000	-0.081	-0.102
Rise	0.000	1.516	6.741	0.000	-0.048	-0.170
Tilt	-0.061	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.081	-0.048	0.000	0.022	0.007
Twist	0.000	-0.102	-0.170	0.000	0.007	0.025

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.902	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.261	1.552	0.000	-0.057	-0.155
Rise	0.000	1.552	7.241	0.000	-0.027	-0.181
Tilt	-0.075	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.057	-0.027	0.000	0.021	0.007
Twist	0.000	-0.155	-0.181	0.000	0.007	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.006	0.000	0.000	-0.041	0.000	0.000
Slide	0.000	1.890	1.451	0.000	-0.074	-0.098
Rise	0.000	1.451	6.634	0.000	-0.046	-0.161
Tilt	-0.041	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.074	-0.046	0.000	0.021	0.008
Twist	0.000	-0.098	-0.161	0.000	0.008	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.007	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	1.938	1.436	0.000	-0.078	-0.099
Rise	0.000	1.436	6.447	0.000	-0.050	-0.164
Tilt	-0.058	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.078	-0.050	0.000	0.022	0.006
Twist	0.000	-0.099	-0.164	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.295	0.000	0.000	0.049	0.000	0.000
Slide	0.000	4.498	3.122	0.000	-0.066	-0.155
Rise	0.000	3.122	9.928	0.000	0.044	-0.134
Tilt	0.049	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.066	0.044	0.000	0.028	0.013
Twist	0.000	-0.155	-0.134	0.000	0.013	0.054

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.295	0.000	0.000	0.053	0.000	0.000
Slide	0.000	4.592	2.796	0.000	-0.077	-0.152
Rise	0.000	2.796	9.397	0.000	0.057	-0.136
Tilt	0.053	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.077	0.057	0.000	0.028	0.013
Twist	0.000	-0.152	-0.136	0.000	0.013	0.054

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.319	0.000	0.000	0.056	0.000	0.000
Slide	0.000	4.388	2.942	0.000	-0.067	-0.147
Rise	0.000	2.942	9.416	0.000	0.046	-0.153
Tilt	0.056	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.067	0.046	0.000	0.027	0.012
Twist	0.000	-0.147	-0.153	0.000	0.012	0.052

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.913	0.000	0.000	-0.072	0.000	0.000
Slide	0.000	2.177	1.492	0.000	-0.054	-0.155
Rise	0.000	1.491	7.321	0.000	-0.022	-0.170
Tilt	-0.072	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.054	-0.022	0.000	0.020	0.007
Twist	0.000	-0.155	-0.170	0.000	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.333	0.000	0.000	-0.038	0.000	0.000
Slide	0.000	2.372	1.509	0.000	-0.052	-0.233
Rise	0.000	1.509	6.997	0.000	-0.059	-0.169
Tilt	-0.038	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.052	-0.059	0.000	0.020	0.013
Twist	0.000	-0.233	-0.169	0.000	0.013	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.300	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	2.575	1.686	0.000	-0.049	-0.248
Rise	0.000	1.686	7.103	0.000	-0.059	-0.192
Tilt	-0.029	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.049	-0.059	0.000	0.019	0.012
Twist	0.000	-0.248	-0.192	0.000	0.012	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.361	0.000	0.000	-0.025	0.000	0.000
Slide	0.000	2.528	1.692	0.000	-0.060	-0.243
Rise	0.000	1.692	7.027	0.000	-0.069	-0.188
Tilt	-0.025	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.060	-0.069	0.000	0.020	0.013
Twist	0.000	-0.243	-0.188	0.000	0.013	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.965	0.000	0.000	0.007	0.000	0.000
Slide	0.000	1.536	1.085	0.000	-0.013	-0.105
Rise	0.000	1.085	6.295	0.000	-0.096	-0.182
Tilt	0.007	0.000	0.000	0.024	0.000	0.000
Roll	0.000	-0.013	-0.096	0.000	0.018	0.013
Twist	0.000	-0.105	-0.181	0.000	0.013	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.912	0.000	0.000	0.002	0.000	0.000
Slide	0.000	1.533	1.024	0.000	0.000	-0.080
Rise	0.000	1.024	6.127	0.000	-0.092	-0.186
Tilt	0.002	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.000	-0.092	0.000	0.017	0.012
Twist	0.000	-0.080	-0.186	0.000	0.012	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.820	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	1.300	0.990	0.000	0.010	-0.065
Rise	0.000	0.990	6.593	0.000	-0.105	-0.193
Tilt	-0.002	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.010	-0.105	0.000	0.017	0.011
Twist	0.000	-0.065	-0.193	0.000	0.011	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.965	0.000	0.000	0.007	0.000	0.000
Slide	0.000	1.479	1.019	0.000	-0.006	-0.096
Rise	0.000	1.019	6.242	0.000	-0.097	-0.181
Tilt	0.007	0.000	0.000	0.024	0.000	0.000
Roll	0.000	-0.006	-0.097	0.000	0.017	0.012
Twist	0.000	-0.096	-0.181	0.000	0.012	0.032

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-agct.4.cte	0.000	0.000

	Mean	Stdev
-agct.8.cte	0.000	0.000

	Mean	Stdev
-atat.10.cte	0.000	0.000

	Mean	Stdev
-atat.14.cte	0.000	0.000

	Mean	Stdev
-atat.6.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gatc.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
-tata.11.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.3.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
rise-10-ATAT	3.224	0.282

	Mean	Stdev
roll-10-ATAT	0.450	5.021

	Mean	Stdev
shift-10-ATAT	0.000	0.690

	Mean	Stdev
slide-10-ATAT	-0.866	0.430

	Mean	Stdev
tilt-10-ATAT	0.000	3.996

	Mean	Stdev
twist-10-ATAT	29.652	3.650

	Mean	Stdev
rise-11-TATA	3.307	0.343

	Mean	Stdev
roll-11-TATA	10.980	7.180

	Mean	Stdev
shift-11-TATA	0.000	0.780

	Mean	Stdev
slide-11-TATA	-0.474	0.736

	Mean	Stdev
tilt-11-TATA	0.000	4.905

	Mean	Stdev
twist-11-TATA	31.463	5.780

	Mean	Stdev
rise-12-AGCT	3.358	0.338

	Mean	Stdev
roll-12-AGCT	5.334	5.624

	Mean	Stdev
shift-12-AGCT	0.000	0.823

	Mean	Stdev
slide-12-AGCT	-0.683	0.669

	Mean	Stdev
tilt-12-AGCT	0.000	4.564

	Mean	Stdev
twist-12-AGCT	31.063	5.723

	Mean	Stdev
rise-13-GATC	3.377	0.313

	Mean	Stdev
roll-13-GATC	3.008	5.961

	Mean	Stdev
shift-13-GATC	0.000	0.676

	Mean	Stdev
slide-13-GATC	-0.634	0.731

	Mean	Stdev
tilt-13-GATC	0.000	4.572

	Mean	Stdev
twist-13-GATC	34.441	5.481

	Mean	Stdev
rise-14-ATAT	3.230	0.287

	Mean	Stdev
roll-14-ATAT	0.291	5.041

	Mean	Stdev
shift-14-ATAT	0.000	0.692

	Mean	Stdev
slide-14-ATAT	-0.862	0.418

	Mean	Stdev
tilt-14-ATAT	0.000	3.892

	Mean	Stdev
twist-14-ATAT	29.758	3.645

	Mean	Stdev
rise-15-TATA	3.325	0.354

	Mean	Stdev
roll-15-TATA	11.043	7.342

	Mean	Stdev
shift-15-TATA	0.000	0.802

	Mean	Stdev
slide-15-TATA	-0.468	0.712

	Mean	Stdev
tilt-15-TATA	0.000	4.916

	Mean	Stdev
twist-15-TATA	31.374	6.128

	Mean	Stdev
rise-16-AGCT	3.334	0.316

	Mean	Stdev
roll-16-AGCT	3.606	5.581

	Mean	Stdev
shift-16-AGCT	0.000	0.908

	Mean	Stdev
slide-16-AGCT	-0.655	0.648

	Mean	Stdev
tilt-16-AGCT	0.000	5.062

	Mean	Stdev
twist-16-AGCT	32.836	5.507

	Mean	Stdev
rise-2-CTAG	3.308	0.311

	Mean	Stdev
roll-2-CTAG	3.043	5.684

	Mean	Stdev
shift-2-CTAG	0.000	0.899

	Mean	Stdev
slide-2-CTAG	-0.624	0.664

	Mean	Stdev
tilt-2-CTAG	0.000	5.154

	Mean	Stdev
twist-2-CTAG	32.852	5.438

	Mean	Stdev
rise-3-TATA	3.223	0.350

	Mean	Stdev
roll-3-TATA	10.881	7.318

	Mean	Stdev
shift-3-TATA	0.000	0.846

	Mean	Stdev
slide-3-TATA	-0.338	0.783

	Mean	Stdev
tilt-3-TATA	0.000	4.899

	Mean	Stdev
twist-3-TATA	30.186	6.630

	Mean	Stdev
rise-4-AGCT	3.337	0.339

	Mean	Stdev
roll-4-AGCT	4.944	5.778

	Mean	Stdev
shift-4-AGCT	0.000	0.785

	Mean	Stdev
slide-4-AGCT	-0.624	0.677

	Mean	Stdev
tilt-4-AGCT	0.000	4.477

	Mean	Stdev
twist-4-AGCT	30.826	5.753

	Mean	Stdev
rise-5-GATC	3.388	0.314

	Mean	Stdev
roll-5-GATC	2.806	6.102

	Mean	Stdev
shift-5-GATC	0.000	0.679

	Mean	Stdev
slide-5-GATC	-0.660	0.711

	Mean	Stdev
tilt-5-GATC	0.000	4.537

	Mean	Stdev
twist-5-GATC	34.403	5.490

	Mean	Stdev
rise-6-ATAT	3.228	0.291

	Mean	Stdev
roll-6-ATAT	0.377	5.119

	Mean	Stdev
shift-6-ATAT	0.000	0.687

	Mean	Stdev
slide-6-ATAT	-0.874	0.432

	Mean	Stdev
tilt-6-ATAT	0.000	3.937

	Mean	Stdev
twist-6-ATAT	29.778	3.736

	Mean	Stdev
rise-7-TATA	3.302	0.346

	Mean	Stdev
roll-7-TATA	10.554	7.277

	Mean	Stdev
shift-7-TATA	0.000	0.780

	Mean	Stdev
slide-7-TATA	-0.469	0.746

	Mean	Stdev
tilt-7-TATA	0.000	4.984

	Mean	Stdev
twist-7-TATA	31.627	5.969

	Mean	Stdev
rise-8-AGCT	3.367	0.343

	Mean	Stdev
roll-8-AGCT	5.139	5.612

	Mean	Stdev
shift-8-AGCT	0.000	0.808

	Mean	Stdev
slide-8-AGCT	-0.686	0.672

	Mean	Stdev
tilt-8-AGCT	0.000	4.611

	Mean	Stdev
twist-8-AGCT	31.173	5.575

	Mean	Stdev
rise-9-GATC	3.370	0.317

	Mean	Stdev
roll-9-GATC	2.961	6.043

	Mean	Stdev
shift-9-GATC	0.000	0.662

	Mean	Stdev
slide-9-GATC	-0.643	0.718

	Mean	Stdev
tilt-9-GATC	0.000	4.528

	Mean	Stdev
twist-9-GATC	34.414	5.574

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