NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.179	0.000	0.000	-0.041	0.000	0.000
Slide	0.000	2.082	1.576	0.000	-0.075	-0.113
Rise	0.000	1.576	7.111	0.000	-0.035	-0.184
Tilt	-0.041	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.075	-0.035	0.000	0.023	0.006
Twist	0.000	-0.113	-0.184	0.000	0.006	0.026

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.350	0.000	0.000	-0.071	0.000	0.000
Slide	0.000	2.516	1.595	0.000	-0.080	-0.136
Rise	0.000	1.595	6.949	0.000	-0.047	-0.189
Tilt	-0.071	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.080	-0.047	0.000	0.024	0.007
Twist	0.000	-0.136	-0.189	0.000	0.007	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.210	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	2.250	1.518	0.000	-0.079	-0.097
Rise	0.000	1.518	6.625	0.000	-0.034	-0.133
Tilt	-0.043	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.079	-0.034	0.000	0.024	0.007
Twist	0.000	-0.097	-0.133	0.000	0.007	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.201	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	2.237	1.656	0.000	-0.077	-0.123
Rise	0.000	1.656	6.819	0.000	-0.042	-0.167
Tilt	-0.036	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.077	-0.042	0.000	0.023	0.006
Twist	0.000	-0.123	-0.167	0.000	0.006	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.350	0.000	0.000	-0.004	0.000	0.000
Slide	0.000	0.665	-0.328	0.000	-0.004	0.030
Rise	0.000	-0.328	3.133	0.000	-0.018	-0.021
Tilt	-0.004	0.000	0.000	0.012	0.000	0.000
Roll	0.000	-0.004	-0.018	0.000	0.006	-0.000
Twist	0.000	0.030	-0.021	0.000	-0.000	0.002

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.089	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	1.753	1.216	0.000	-0.042	-0.142
Rise	0.000	1.216	7.637	0.000	-0.040	-0.155
Tilt	-0.051	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.042	-0.040	0.000	0.021	0.012
Twist	0.000	-0.142	-0.155	0.000	0.012	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.188	0.000	0.000	-0.068	0.000	0.000
Slide	0.000	1.832	1.162	0.000	-0.024	-0.137
Rise	0.000	1.162	7.214	0.000	-0.017	-0.162
Tilt	-0.068	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.024	-0.017	0.000	0.021	0.011
Twist	0.000	-0.137	-0.162	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.029	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	0.812	1.077	0.000	-0.003	-0.014
Rise	0.000	1.077	7.143	0.000	-0.050	-0.159
Tilt	-0.103	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.003	-0.050	0.000	0.022	0.008
Twist	0.000	-0.014	-0.159	0.000	0.008	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.084	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	1.717	1.243	0.000	-0.042	-0.134
Rise	0.000	1.243	7.979	0.000	-0.042	-0.165
Tilt	-0.056	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.042	-0.042	0.000	0.022	0.012
Twist	0.000	-0.134	-0.165	0.000	0.012	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.359	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	1.487	1.690	0.000	-0.018	-0.089
Rise	0.000	1.690	6.879	0.000	-0.051	-0.177
Tilt	-0.066	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.018	-0.051	0.000	0.020	0.007
Twist	0.000	-0.089	-0.177	0.000	0.007	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.299	0.000	0.000	-0.073	0.000	0.000
Slide	0.000	1.777	1.612	0.000	-0.015	-0.151
Rise	0.000	1.612	7.542	0.000	-0.018	-0.194
Tilt	-0.073	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.015	-0.018	0.000	0.020	0.009
Twist	0.000	-0.151	-0.194	0.000	0.009	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.331	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	1.438	1.790	0.000	-0.026	-0.082
Rise	0.000	1.790	7.225	0.000	-0.051	-0.190
Tilt	-0.081	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.026	-0.051	0.000	0.021	0.008
Twist	0.000	-0.082	-0.190	0.000	0.008	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.431	0.000	0.000	-0.087	0.000	0.000
Slide	0.000	1.888	1.714	0.000	-0.021	-0.155
Rise	0.000	1.714	6.943	0.000	-0.007	-0.176
Tilt	-0.087	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.021	-0.007	0.000	0.020	0.008
Twist	0.000	-0.155	-0.176	0.000	0.008	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.331	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	1.413	1.735	0.000	-0.015	-0.082
Rise	0.000	1.735	7.235	0.000	-0.038	-0.189
Tilt	-0.077	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.015	-0.038	0.000	0.019	0.007
Twist	0.000	-0.082	-0.189	0.000	0.007	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.407	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	1.872	1.674	0.000	-0.021	-0.152
Rise	0.000	1.674	7.099	0.000	-0.016	-0.168
Tilt	-0.074	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.021	-0.016	0.000	0.021	0.008
Twist	0.000	-0.152	-0.168	0.000	0.008	0.034

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-agct.4.cte	0.000	0.000

	Mean	Stdev
-agct.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.13.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.9.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.442	0.355

	Mean	Stdev
roll-10-GGCC	6.479	5.714

	Mean	Stdev
shift-10-GGCC	0.000	0.691

	Mean	Stdev
slide-10-GGCC	-0.914	0.780

	Mean	Stdev
tilt-10-GGCC	0.000	4.450

	Mean	Stdev
twist-10-GGCC	30.484	5.974

	Mean	Stdev
rise-11-GATC	3.211	0.297

	Mean	Stdev
roll-11-GATC	3.303	5.896

	Mean	Stdev
shift-11-GATC	0.000	0.768

	Mean	Stdev
slide-11-GATC	-0.374	0.744

	Mean	Stdev
tilt-11-GATC	0.000	4.842

	Mean	Stdev
twist-11-GATC	33.405	6.088

	Mean	Stdev
rise-12-AGCT	3.235	0.330

	Mean	Stdev
roll-12-AGCT	3.881	5.417

	Mean	Stdev
shift-12-AGCT	0.000	0.723

	Mean	Stdev
slide-12-AGCT	-0.755	0.652

	Mean	Stdev
tilt-12-AGCT	0.000	4.588

	Mean	Stdev
twist-12-AGCT	29.803	5.638

	Mean	Stdev
rise-13-GGCC	3.429	0.315

	Mean	Stdev
roll-13-GGCC	5.474	5.748

	Mean	Stdev
shift-13-GGCC	0.000	0.716

	Mean	Stdev
slide-13-GGCC	-0.835	0.762

	Mean	Stdev
tilt-13-GGCC	0.000	4.383

	Mean	Stdev
twist-13-GGCC	32.707	5.609

	Mean	Stdev
rise-14-GGCC	3.404	0.357

	Mean	Stdev
roll-14-GGCC	6.769	5.685

	Mean	Stdev
shift-14-GGCC	0.000	0.718

	Mean	Stdev
slide-14-GGCC	-0.766	0.794

	Mean	Stdev
tilt-14-GGCC	0.000	4.471

	Mean	Stdev
twist-14-GGCC	30.338	5.997

	Mean	Stdev
rise-15-GATC	3.300	0.309

	Mean	Stdev
roll-15-GATC	2.117	5.915

	Mean	Stdev
shift-15-GATC	0.000	0.757

	Mean	Stdev
slide-15-GATC	-0.312	0.715

	Mean	Stdev
tilt-15-GATC	0.000	4.892

	Mean	Stdev
twist-15-GATC	34.744	6.241

	Mean	Stdev
rise-16-AGCT	3.379	0.327

	Mean	Stdev
roll-16-AGCT	2.719	5.265

	Mean	Stdev
shift-16-AGCT	0.000	0.705

	Mean	Stdev
slide-16-AGCT	-0.762	0.586

	Mean	Stdev
tilt-16-AGCT	0.000	4.774

	Mean	Stdev
twist-16-AGCT	32.915	5.209

	Mean	Stdev
rise-2-CGCG	3.148	0.452

	Mean	Stdev
roll-2-CGCG	4.507	10.105

	Mean	Stdev
shift-2-CGCG	0.000	1.296

	Mean	Stdev
slide-2-CGCG	1.230	1.394

	Mean	Stdev
tilt-2-CGCG	0.000	7.097

	Mean	Stdev
twist-2-CGCG	9.784	23.074

	Mean	Stdev
rise-3-GATC	3.201	0.353

	Mean	Stdev
roll-3-GATC	1.777	5.625

	Mean	Stdev
shift-3-GATC	0.000	1.043

	Mean	Stdev
slide-3-GATC	0.543	0.955

	Mean	Stdev
tilt-3-GATC	0.000	7.206

	Mean	Stdev
twist-3-GATC	33.913	6.410

	Mean	Stdev
rise-4-AGCT	3.370	0.330

	Mean	Stdev
roll-4-AGCT	3.735	5.361

	Mean	Stdev
shift-4-AGCT	0.000	0.714

	Mean	Stdev
slide-4-AGCT	-0.683	0.607

	Mean	Stdev
tilt-4-AGCT	0.000	4.487

	Mean	Stdev
twist-4-AGCT	31.737	4.948

	Mean	Stdev
rise-5-GGCC	3.507	0.332

	Mean	Stdev
roll-5-GGCC	5.764	5.711

	Mean	Stdev
shift-5-GGCC	0.000	0.698

	Mean	Stdev
slide-5-GGCC	-0.938	0.741

	Mean	Stdev
tilt-5-GGCC	0.000	4.564

	Mean	Stdev
twist-5-GGCC	32.872	5.286

	Mean	Stdev
rise-6-GGCC	3.425	0.352

	Mean	Stdev
roll-6-GGCC	6.494	5.900

	Mean	Stdev
shift-6-GGCC	0.000	0.715

	Mean	Stdev
slide-6-GGCC	-0.855	0.794

	Mean	Stdev
tilt-6-GGCC	0.000	4.579

	Mean	Stdev
twist-6-GGCC	30.596	5.833

	Mean	Stdev
rise-7-GATC	3.206	0.293

	Mean	Stdev
roll-7-GATC	3.081	5.857

	Mean	Stdev
shift-7-GATC	0.000	0.778

	Mean	Stdev
slide-7-GATC	-0.377	0.740

	Mean	Stdev
tilt-7-GATC	0.000	4.922

	Mean	Stdev
twist-7-GATC	33.433	6.211

	Mean	Stdev
rise-8-AGCT	3.262	0.332

	Mean	Stdev
roll-8-AGCT	3.600	5.454

	Mean	Stdev
shift-8-AGCT	0.000	0.712

	Mean	Stdev
slide-8-AGCT	-0.813	0.640

	Mean	Stdev
tilt-8-AGCT	0.000	4.576

	Mean	Stdev
twist-8-AGCT	30.182	5.376

	Mean	Stdev
rise-9-GGCC	3.458	0.325

	Mean	Stdev
roll-9-GGCC	5.729	5.608

	Mean	Stdev
shift-9-GGCC	0.000	0.686

	Mean	Stdev
slide-9-GGCC	-0.986	0.745

	Mean	Stdev
tilt-9-GGCC	0.000	4.425

	Mean	Stdev
twist-9-GGCC	32.154	5.429

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