NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.227	0.000	0.000	-0.130	0.000	0.000
Slide	0.000	1.127	0.695	0.000	0.022	-0.001
Rise	0.000	0.695	5.447	0.000	-0.052	-0.123
Tilt	-0.130	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.022	-0.052	0.000	0.015	0.004
Twist	0.000	-0.001	-0.123	0.000	0.004	0.013

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.880	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.858	1.745	0.000	-0.026	-0.210
Rise	0.000	1.745	6.586	0.000	-0.036	-0.185
Tilt	-0.077	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.026	-0.036	0.000	0.024	0.008
Twist	0.000	-0.210	-0.185	0.000	0.008	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.121	0.000	0.000	-0.097	0.000	0.000
Slide	0.000	1.970	1.657	0.000	-0.009	-0.163
Rise	0.000	1.657	8.179	0.000	-0.001	-0.165
Tilt	-0.097	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.009	-0.001	0.000	0.021	0.006
Twist	0.000	-0.163	-0.165	0.000	0.006	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.795	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	2.784	1.805	0.000	-0.027	-0.200
Rise	0.000	1.805	6.670	0.000	-0.049	-0.181
Tilt	-0.079	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.027	-0.049	0.000	0.024	0.008
Twist	0.000	-0.200	-0.181	0.000	0.008	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.223	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	1.804	1.623	0.000	-0.002	-0.153
Rise	0.000	1.623	7.748	0.000	-0.011	-0.173
Tilt	-0.107	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.002	-0.011	0.000	0.021	0.008
Twist	0.000	-0.153	-0.173	0.000	0.008	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.938	0.000	0.000	-0.087	0.000	0.000
Slide	0.000	2.772	1.845	0.000	-0.031	-0.200
Rise	0.000	1.844	6.811	0.000	-0.043	-0.183
Tilt	-0.087	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.031	-0.043	0.000	0.025	0.009
Twist	0.000	-0.200	-0.183	0.000	0.009	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.144	0.000	0.000	-0.102	0.000	0.000
Slide	0.000	1.869	1.470	0.000	-0.000	-0.164
Rise	0.000	1.470	8.053	0.000	-0.009	-0.161
Tilt	-0.102	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.000	-0.009	0.000	0.022	0.007
Twist	0.000	-0.164	-0.161	0.000	0.007	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.211	0.000	0.000	-0.032	0.000	0.000
Slide	0.000	3.790	2.910	0.000	0.049	-0.191
Rise	0.000	2.910	11.132	0.000	0.163	-0.203
Tilt	-0.032	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.049	0.163	0.000	0.022	0.008
Twist	0.000	-0.191	-0.203	0.000	0.008	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.215	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	3.604	2.696	0.000	0.016	-0.164
Rise	0.000	2.696	10.027	0.000	0.090	-0.212
Tilt	-0.029	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.016	0.090	0.000	0.024	0.009
Twist	0.000	-0.164	-0.212	0.000	0.009	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.985	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	3.474	2.304	0.000	0.039	-0.109
Rise	0.000	2.304	9.744	0.000	0.116	-0.218
Tilt	-0.053	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.039	0.116	0.000	0.021	0.011
Twist	0.000	-0.109	-0.218	0.000	0.011	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.190	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	3.585	2.798	0.000	0.038	-0.171
Rise	0.000	2.798	10.844	0.000	0.155	-0.200
Tilt	-0.029	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.038	0.155	0.000	0.024	0.010
Twist	0.000	-0.171	-0.200	0.000	0.010	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.053	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	2.521	0.545	0.000	0.008	-0.050
Rise	0.000	0.545	8.487	0.000	-0.143	-0.230
Tilt	-0.023	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.008	-0.143	0.000	0.022	0.008
Twist	0.000	-0.050	-0.230	0.000	0.008	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.230	0.000	0.000	0.017	0.000	0.000
Slide	0.000	0.990	-0.065	0.000	0.001	-0.027
Rise	0.000	-0.064	2.082	0.000	-0.031	-0.093
Tilt	0.017	0.000	0.000	0.005	0.000	0.000
Roll	0.000	0.001	-0.031	0.000	0.004	-0.001
Twist	0.000	-0.027	-0.093	0.000	-0.001	0.010

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.953	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	2.321	0.601	0.000	0.023	-0.059
Rise	0.000	0.601	8.106	0.000	-0.125	-0.249
Tilt	-0.037	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.023	-0.125	0.000	0.022	0.009
Twist	0.000	-0.059	-0.249	0.000	0.009	0.018

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.069	0.000	0.000	-0.013	0.000	0.000
Slide	0.000	2.505	0.580	0.000	0.013	-0.043
Rise	0.000	0.580	8.418	0.000	-0.135	-0.235
Tilt	-0.013	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.013	-0.135	0.000	0.022	0.007
Twist	0.000	-0.043	-0.235	0.000	0.007	0.017

[Image courtesy of Curves+]

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	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.13.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.9.cte	0.000	0.000

	Mean	Stdev
-gtac.11.cte	0.000	0.000

	Mean	Stdev
-gtac.15.cte	0.000	0.000

	Mean	Stdev
-gtac.3.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tgca.12.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
-tgca.4.cte	0.000	0.000

	Mean	Stdev
-tgca.8.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.677	0.330

	Mean	Stdev
roll-10-GGCC	5.490	5.138

	Mean	Stdev
shift-10-GGCC	0.000	0.583

	Mean	Stdev
slide-10-GGCC	-1.225	0.594

	Mean	Stdev
tilt-10-GGCC	0.000	4.127

	Mean	Stdev
twist-10-GGCC	33.717	5.010

	Mean	Stdev
rise-11-GTAC	3.289	0.277

	Mean	Stdev
roll-11-GTAC	-1.925	6.088

	Mean	Stdev
shift-11-GTAC	0.000	0.703

	Mean	Stdev
slide-11-GTAC	-0.511	0.493

	Mean	Stdev
tilt-11-GTAC	0.000	3.907

	Mean	Stdev
twist-11-GTAC	33.577	4.746

	Mean	Stdev
rise-12-TGCA	2.917	0.331

	Mean	Stdev
roll-12-TGCA	9.455	5.701

	Mean	Stdev
shift-12-TGCA	0.000	0.753

	Mean	Stdev
slide-12-TGCA	-0.167	0.502

	Mean	Stdev
tilt-12-TGCA	0.000	4.601

	Mean	Stdev
twist-12-TGCA	25.185	7.859

	Mean	Stdev
rise-13-GGCC	3.437	0.295

	Mean	Stdev
roll-13-GGCC	3.970	5.603

	Mean	Stdev
shift-13-GGCC	0.000	0.818

	Mean	Stdev
slide-13-GGCC	-0.558	0.774

	Mean	Stdev
tilt-13-GGCC	0.000	4.426

	Mean	Stdev
twist-13-GGCC	33.709	6.263

	Mean	Stdev
rise-14-GGCC	3.681	0.330

	Mean	Stdev
roll-14-GGCC	5.847	5.143

	Mean	Stdev
shift-14-GGCC	0.000	0.599

	Mean	Stdev
slide-14-GGCC	-1.225	0.600

	Mean	Stdev
tilt-14-GGCC	0.000	4.108

	Mean	Stdev
twist-14-GGCC	33.440	5.042

	Mean	Stdev
rise-15-GTAC	3.316	0.285

	Mean	Stdev
roll-15-GTAC	-0.311	5.588

	Mean	Stdev
shift-15-GTAC	0.000	0.701

	Mean	Stdev
slide-15-GTAC	-0.644	0.483

	Mean	Stdev
tilt-15-GTAC	0.000	3.882

	Mean	Stdev
twist-15-GTAC	32.529	4.794

	Mean	Stdev
rise-16-TGCA	2.987	0.990

	Mean	Stdev
roll-16-TGCA	3.599	16.062

	Mean	Stdev
shift-16-TGCA	0.000	1.871

	Mean	Stdev
slide-16-TGCA	-0.227	0.890

	Mean	Stdev
tilt-16-TGCA	0.028	13.301

	Mean	Stdev
twist-16-TGCA	27.393	14.471

	Mean	Stdev
rise-2-CGCG	3.460	0.394

	Mean	Stdev
roll-2-CGCG	7.554	6.682

	Mean	Stdev
shift-2-CGCG	0.000	0.904

	Mean	Stdev
slide-2-CGCG	-0.010	0.776

	Mean	Stdev
tilt-2-CGCG	0.000	5.503

	Mean	Stdev
twist-2-CGCG	37.210	7.987

	Mean	Stdev
rise-3-GTAC	3.310	0.312

	Mean	Stdev
roll-3-GTAC	-2.375	6.791

	Mean	Stdev
shift-3-GTAC	0.000	0.804

	Mean	Stdev
slide-3-GTAC	-0.349	0.467

	Mean	Stdev
tilt-3-GTAC	0.000	4.152

	Mean	Stdev
twist-3-GTAC	31.900	5.959

	Mean	Stdev
rise-4-TGCA	2.991	0.353

	Mean	Stdev
roll-4-TGCA	9.066	5.973

	Mean	Stdev
shift-4-TGCA	0.000	0.805

	Mean	Stdev
slide-4-TGCA	-0.120	0.544

	Mean	Stdev
tilt-4-TGCA	0.000	4.737

	Mean	Stdev
twist-4-TGCA	27.572	8.338

	Mean	Stdev
rise-5-GGCC	3.476	0.307

	Mean	Stdev
roll-5-GGCC	4.552	5.594

	Mean	Stdev
shift-5-GGCC	0.000	0.791

	Mean	Stdev
slide-5-GGCC	-0.600	0.776

	Mean	Stdev
tilt-5-GGCC	0.000	4.364

	Mean	Stdev
twist-5-GGCC	33.918	5.607

	Mean	Stdev
rise-6-GGCC	3.663	0.327

	Mean	Stdev
roll-6-GGCC	5.519	5.098

	Mean	Stdev
shift-6-GGCC	0.000	0.580

	Mean	Stdev
slide-6-GGCC	-1.222	0.603

	Mean	Stdev
tilt-6-GGCC	0.000	4.123

	Mean	Stdev
twist-6-GGCC	33.554	5.030

	Mean	Stdev
rise-7-GTAC	3.276	0.283

	Mean	Stdev
roll-7-GTAC	-1.825	6.013

	Mean	Stdev
shift-7-GTAC	0.000	0.708

	Mean	Stdev
slide-7-GTAC	-0.508	0.494

	Mean	Stdev
tilt-7-GTAC	0.000	3.917

	Mean	Stdev
twist-7-GTAC	33.230	4.827

	Mean	Stdev
rise-8-TGCA	2.927	0.338

	Mean	Stdev
roll-8-TGCA	9.489	5.768

	Mean	Stdev
shift-8-TGCA	0.000	0.742

	Mean	Stdev
slide-8-TGCA	-0.159	0.500

	Mean	Stdev
tilt-8-TGCA	0.000	4.524

	Mean	Stdev
twist-8-TGCA	25.339	7.904

	Mean	Stdev
rise-9-GGCC	3.448	0.293

	Mean	Stdev
roll-9-GGCC	3.814	5.537

	Mean	Stdev
shift-9-GGCC	0.000	0.818

	Mean	Stdev
slide-9-GGCC	-0.527	0.781

	Mean	Stdev
tilt-9-GGCC	0.000	4.441

	Mean	Stdev
twist-9-GGCC	34.144	5.832

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