NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.924	0.000	0.000	-0.099	0.000	0.000
Slide	0.000	2.089	1.323	0.000	-0.042	-0.125
Rise	0.000	1.323	7.130	0.000	-0.029	-0.179
Tilt	-0.099	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.042	-0.029	0.000	0.019	0.007
Twist	0.000	-0.125	-0.179	0.000	0.007	0.029

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.338	0.000	0.000	0.036	0.000	0.000
Slide	0.000	4.157	3.208	0.000	-0.050	-0.099
Rise	0.000	3.208	10.532	0.000	0.042	-0.183
Tilt	0.036	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.050	0.042	0.000	0.028	0.013
Twist	0.000	-0.099	-0.183	0.000	0.013	0.042

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.303	0.000	0.000	0.036	0.000	0.000
Slide	0.000	3.770	2.781	0.000	-0.053	-0.092
Rise	0.000	2.781	9.764	0.000	0.067	-0.151
Tilt	0.036	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.053	0.067	0.000	0.027	0.013
Twist	0.000	-0.092	-0.151	0.000	0.013	0.039

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.147	0.000	0.000	0.036	0.000	0.000
Slide	0.000	3.971	2.717	0.000	-0.047	-0.066
Rise	0.000	2.717	10.064	0.000	0.055	-0.152
Tilt	0.036	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.047	0.055	0.000	0.026	0.011
Twist	0.000	-0.066	-0.152	0.000	0.011	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.191	0.000	0.000	0.039	0.000	0.000
Slide	0.000	3.981	2.867	0.000	-0.054	-0.081
Rise	0.000	2.867	10.356	0.000	0.039	-0.170
Tilt	0.039	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.054	0.039	0.000	0.027	0.014
Twist	0.000	-0.081	-0.170	0.000	0.014	0.038

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.385	0.000	0.000	0.033	0.000	0.000
Slide	0.000	3.888	2.737	0.000	-0.058	-0.092
Rise	0.000	2.737	9.907	0.000	0.060	-0.149
Tilt	0.033	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.058	0.060	0.000	0.028	0.013
Twist	0.000	-0.092	-0.149	0.000	0.013	0.043

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.346	0.000	0.000	0.033	0.000	0.000
Slide	0.000	3.872	2.667	0.000	-0.079	-0.082
Rise	0.000	2.667	9.503	0.000	0.023	-0.174
Tilt	0.033	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.079	0.023	0.000	0.027	0.011
Twist	0.000	-0.082	-0.174	0.000	0.011	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.949	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	2.184	1.447	0.000	-0.049	-0.132
Rise	0.000	1.447	7.219	0.000	-0.020	-0.188
Tilt	-0.103	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.049	-0.020	0.000	0.020	0.007
Twist	0.000	-0.132	-0.188	0.000	0.007	0.029

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.929	0.000	0.000	-0.006	0.000	0.000
Slide	0.000	1.844	1.011	0.000	-0.002	-0.115
Rise	0.000	1.011	5.646	0.000	-0.071	-0.182
Tilt	-0.006	0.000	0.000	0.023	0.000	0.000
Roll	0.000	-0.002	-0.071	0.000	0.015	0.011
Twist	0.000	-0.115	-0.182	0.000	0.011	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.861	0.000	0.000	-0.006	0.000	0.000
Slide	0.000	1.837	1.055	0.000	-0.001	-0.115
Rise	0.000	1.055	5.902	0.000	-0.070	-0.193
Tilt	-0.006	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.001	-0.070	0.000	0.016	0.012
Twist	0.000	-0.115	-0.193	0.000	0.012	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.712	0.000	0.000	-0.016	0.000	0.000
Slide	0.000	1.368	0.776	0.000	0.019	-0.046
Rise	0.000	0.776	6.253	0.000	-0.086	-0.195
Tilt	-0.016	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.019	-0.086	0.000	0.017	0.011
Twist	0.000	-0.046	-0.195	0.000	0.011	0.022

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.689	0.000	0.000	-0.020	0.000	0.000
Slide	0.000	1.528	0.874	0.000	0.011	-0.072
Rise	0.000	0.874	6.075	0.000	-0.087	-0.201
Tilt	-0.020	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.011	-0.087	0.000	0.016	0.011
Twist	0.000	-0.072	-0.201	0.000	0.011	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.919	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	1.925	1.061	0.000	-0.002	-0.121
Rise	0.000	1.061	5.777	0.000	-0.070	-0.185
Tilt	-0.009	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.002	-0.070	0.000	0.015	0.011
Twist	0.000	-0.121	-0.185	0.000	0.011	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.925	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	2.063	1.221	0.000	-0.002	-0.138
Rise	0.000	1.221	5.718	0.000	-0.061	-0.199
Tilt	-0.009	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.002	-0.061	0.000	0.015	0.011
Twist	0.000	-0.138	-0.199	0.000	0.011	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.896	0.000	0.000	-0.007	0.000	0.000
Slide	0.000	1.976	1.054	0.000	-0.002	-0.124
Rise	0.000	1.054	5.583	0.000	-0.069	-0.176
Tilt	-0.007	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.002	-0.069	0.000	0.015	0.011
Twist	0.000	-0.124	-0.176	0.000	0.011	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.10.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.14.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-atat.6.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-tata.11.cte	0.000	0.000

	Mean	Stdev
-tata.13.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.3.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
rise-10-ATAT	3.218	0.285

	Mean	Stdev
roll-10-ATAT	0.151	5.157

	Mean	Stdev
shift-10-ATAT	0.000	0.670

	Mean	Stdev
slide-10-ATAT	-0.864	0.440

	Mean	Stdev
tilt-10-ATAT	0.000	3.880

	Mean	Stdev
twist-10-ATAT	29.607	4.291

	Mean	Stdev
rise-11-TATA	3.406	0.362

	Mean	Stdev
roll-11-TATA	8.567	7.519

	Mean	Stdev
shift-11-TATA	0.000	0.795

	Mean	Stdev
slide-11-TATA	-0.572	0.680

	Mean	Stdev
tilt-11-TATA	0.000	5.025

	Mean	Stdev
twist-11-TATA	34.446	6.154

	Mean	Stdev
rise-12-ATAT	3.218	0.294

	Mean	Stdev
roll-12-ATAT	0.150	5.386

	Mean	Stdev
shift-12-ATAT	0.000	0.680

	Mean	Stdev
slide-12-ATAT	-0.858	0.460

	Mean	Stdev
tilt-12-ATAT	0.000	3.882

	Mean	Stdev
twist-12-ATAT	29.577	4.440

	Mean	Stdev
rise-13-TATA	3.367	0.355

	Mean	Stdev
roll-13-TATA	8.316	7.509

	Mean	Stdev
shift-13-TATA	0.000	0.826

	Mean	Stdev
slide-13-TATA	-0.551	0.683

	Mean	Stdev
tilt-13-TATA	0.000	5.186

	Mean	Stdev
twist-13-TATA	34.391	6.139

	Mean	Stdev
rise-14-ATAT	3.189	0.285

	Mean	Stdev
roll-14-ATAT	0.703	5.395

	Mean	Stdev
shift-14-ATAT	0.000	0.726

	Mean	Stdev
slide-14-ATAT	-0.798	0.436

	Mean	Stdev
tilt-14-ATAT	0.000	3.999

	Mean	Stdev
twist-14-ATAT	29.008	4.663

	Mean	Stdev
rise-15-TATA	3.267	0.363

	Mean	Stdev
roll-15-TATA	9.144	7.687

	Mean	Stdev
shift-15-TATA	0.000	0.915

	Mean	Stdev
slide-15-TATA	-0.334	0.731

	Mean	Stdev
tilt-15-TATA	0.000	5.098

	Mean	Stdev
twist-15-TATA	31.656	7.401

	Mean	Stdev
rise-16-AGCT	3.376	0.318

	Mean	Stdev
roll-16-AGCT	3.033	5.809

	Mean	Stdev
shift-16-AGCT	0.000	0.987

	Mean	Stdev
slide-16-AGCT	-0.504	0.627

	Mean	Stdev
tilt-16-AGCT	0.000	5.454

	Mean	Stdev
twist-16-AGCT	34.538	5.534

	Mean	Stdev
rise-2-CTAG	3.381	0.320

	Mean	Stdev
roll-2-CTAG	2.903	5.666

	Mean	Stdev
shift-2-CTAG	0.000	0.981

	Mean	Stdev
slide-2-CTAG	-0.551	0.625

	Mean	Stdev
tilt-2-CTAG	0.000	5.392

	Mean	Stdev
twist-2-CTAG	34.405	5.670

	Mean	Stdev
rise-3-TATA	3.276	0.365

	Mean	Stdev
roll-3-TATA	8.999	7.645

	Mean	Stdev
shift-3-TATA	0.000	0.934

	Mean	Stdev
slide-3-TATA	-0.357	0.717

	Mean	Stdev
tilt-3-TATA	0.000	5.118

	Mean	Stdev
twist-3-TATA	31.919	7.083

	Mean	Stdev
rise-4-ATAT	3.186	0.283

	Mean	Stdev
roll-4-ATAT	0.504	5.329

	Mean	Stdev
shift-4-ATAT	0.000	0.714

	Mean	Stdev
slide-4-ATAT	-0.803	0.439

	Mean	Stdev
tilt-4-ATAT	0.000	3.965

	Mean	Stdev
twist-4-ATAT	29.224	4.628

	Mean	Stdev
rise-5-TATA	3.369	0.358

	Mean	Stdev
roll-5-TATA	8.293	7.751

	Mean	Stdev
shift-5-TATA	0.000	0.800

	Mean	Stdev
slide-5-TATA	-0.556	0.675

	Mean	Stdev
tilt-5-TATA	0.000	5.128

	Mean	Stdev
twist-5-TATA	34.436	6.233

	Mean	Stdev
rise-6-ATAT	3.222	0.286

	Mean	Stdev
roll-6-ATAT	0.080	5.212

	Mean	Stdev
shift-6-ATAT	0.000	0.657

	Mean	Stdev
slide-6-ATAT	-0.870	0.448

	Mean	Stdev
tilt-6-ATAT	0.000	3.861

	Mean	Stdev
twist-6-ATAT	29.568	4.182

	Mean	Stdev
rise-7-TATA	3.408	0.365

	Mean	Stdev
roll-7-TATA	8.193	7.631

	Mean	Stdev
shift-7-TATA	0.000	0.797

	Mean	Stdev
slide-7-TATA	-0.575	0.675

	Mean	Stdev
tilt-7-TATA	0.000	5.214

	Mean	Stdev
twist-7-TATA	34.671	6.313

	Mean	Stdev
rise-8-ATAT	3.203	0.296

	Mean	Stdev
roll-8-ATAT	0.405	5.276

	Mean	Stdev
shift-8-ATAT	0.000	0.667

	Mean	Stdev
slide-8-ATAT	-0.846	0.453

	Mean	Stdev
tilt-8-ATAT	0.000	3.952

	Mean	Stdev
twist-8-ATAT	29.358	4.689

	Mean	Stdev
rise-9-TATA	3.370	0.362

	Mean	Stdev
roll-9-TATA	8.244	7.747

	Mean	Stdev
shift-9-TATA	0.000	0.810

	Mean	Stdev
slide-9-TATA	-0.550	0.673

	Mean	Stdev
tilt-9-TATA	0.000	5.184

	Mean	Stdev
twist-9-TATA	34.401	6.272

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	A	-	T	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19