NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.267	0.000	0.000	-0.092	0.000	0.000
Slide	0.000	2.495	1.930	0.000	-0.054	-0.121
Rise	0.000	1.930	7.722	0.000	-0.027	-0.174
Tilt	-0.092	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.054	-0.027	0.000	0.024	0.005
Twist	0.000	-0.121	-0.174	0.000	0.005	0.030

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.312	0.000	0.000	-0.032	0.000	0.000
Slide	0.000	5.547	3.041	0.000	-0.091	-0.120
Rise	0.000	3.041	10.902	0.000	0.023	-0.177
Tilt	-0.032	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.091	0.023	0.000	0.033	0.016
Twist	0.000	-0.120	-0.177	0.000	0.016	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.136	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	5.728	3.325	0.000	-0.068	-0.117
Rise	0.000	3.325	11.177	0.000	0.041	-0.171
Tilt	-0.026	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.068	0.041	0.000	0.031	0.014
Twist	0.000	-0.117	-0.171	0.000	0.014	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.948	0.000	0.000	-0.012	0.000	0.000
Slide	0.000	5.716	3.211	0.000	-0.088	-0.113
Rise	0.000	3.211	11.158	0.000	0.045	-0.164
Tilt	-0.012	0.000	0.000	0.047	0.000	0.000
Roll	0.000	-0.088	0.045	0.000	0.033	0.014
Twist	0.000	-0.113	-0.164	0.000	0.014	0.049

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.112	0.000	0.000	-0.117	0.000	0.000
Slide	0.000	3.418	0.418	0.000	0.007	-0.138
Rise	0.000	0.418	8.338	0.000	-0.128	-0.270
Tilt	-0.117	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.007	-0.128	0.000	0.020	0.009
Twist	0.000	-0.138	-0.270	0.000	0.009	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.104	0.000	0.000	-0.111	0.000	0.000
Slide	0.000	3.032	0.596	0.000	-0.003	-0.133
Rise	0.000	0.596	8.824	0.000	-0.142	-0.275
Tilt	-0.111	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.003	-0.142	0.000	0.022	0.009
Twist	0.000	-0.133	-0.275	0.000	0.009	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.094	0.000	0.000	-0.141	0.000	0.000
Slide	0.000	3.037	0.813	0.000	0.021	-0.130
Rise	0.000	0.813	8.094	0.000	-0.077	-0.243
Tilt	-0.141	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.021	-0.077	0.000	0.018	0.007
Twist	0.000	-0.130	-0.243	0.000	0.007	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.124	0.000	0.000	-0.116	0.000	0.000
Slide	0.000	3.250	0.604	0.000	0.000	-0.135
Rise	0.000	0.604	8.440	0.000	-0.128	-0.279
Tilt	-0.116	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.000	-0.128	0.000	0.020	0.009
Twist	0.000	-0.135	-0.279	0.000	0.009	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.877	0.000	0.000	-0.108	0.000	0.000
Slide	0.000	2.299	1.509	0.000	-0.037	-0.096
Rise	0.000	1.509	8.738	0.000	-0.039	-0.179
Tilt	-0.108	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.037	-0.039	0.000	0.022	0.010
Twist	0.000	-0.096	-0.179	0.000	0.010	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.916	0.000	0.000	-0.097	0.000	0.000
Slide	0.000	2.257	1.028	0.000	-0.030	-0.131
Rise	0.000	1.028	8.306	0.000	-0.025	-0.168
Tilt	-0.097	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.030	-0.025	0.000	0.023	0.010
Twist	0.000	-0.131	-0.168	0.000	0.010	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.933	0.000	0.000	-0.127	0.000	0.000
Slide	0.000	2.127	1.782	0.000	-0.040	-0.086
Rise	0.000	1.782	8.992	0.000	-0.035	-0.206
Tilt	-0.127	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.040	-0.035	0.000	0.025	0.013
Twist	0.000	-0.086	-0.206	0.000	0.013	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.938	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	2.138	1.634	0.000	-0.042	-0.116
Rise	0.000	1.634	8.486	0.000	-0.037	-0.180
Tilt	-0.100	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.042	-0.037	0.000	0.022	0.011
Twist	0.000	-0.116	-0.180	0.000	0.011	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.844	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	2.349	1.682	0.000	-0.029	-0.108
Rise	0.000	1.682	8.471	0.000	-0.043	-0.159
Tilt	-0.105	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.029	-0.043	0.000	0.022	0.008
Twist	0.000	-0.108	-0.159	0.000	0.008	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.942	0.000	0.000	-0.108	0.000	0.000
Slide	0.000	2.183	1.742	0.000	-0.048	-0.118
Rise	0.000	1.742	8.705	0.000	-0.030	-0.193
Tilt	-0.108	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.048	-0.030	0.000	0.022	0.010
Twist	0.000	-0.118	-0.193	0.000	0.010	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.924	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.121	1.590	0.000	-0.048	-0.113
Rise	0.000	1.590	8.132	0.000	-0.035	-0.178
Tilt	-0.094	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.048	-0.035	0.000	0.022	0.011
Twist	0.000	-0.113	-0.178	0.000	0.011	0.040

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.10.cte	0.000	0.000

	Mean	Stdev
-cgcg.14.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.6.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-tcga.13.cte	0.000	0.000

	Mean	Stdev
-tcga.5.cte	0.000	0.000

	Mean	Stdev
-tcga.9.cte	0.000	0.000

	Mean	Stdev
rise-10-CGCG	3.106	0.339

	Mean	Stdev
roll-10-CGCG	8.203	6.162

	Mean	Stdev
shift-10-CGCG	0.000	0.917

	Mean	Stdev
slide-10-CGCG	0.035	0.501

	Mean	Stdev
tilt-10-CGCG	0.000	5.328

	Mean	Stdev
twist-10-CGCG	29.319	7.414

	Mean	Stdev
rise-11-GATC	3.422	0.282

	Mean	Stdev
roll-11-GATC	1.274	5.454

	Mean	Stdev
shift-11-GATC	0.000	1.035

	Mean	Stdev
slide-11-GATC	0.089	0.555

	Mean	Stdev
tilt-11-GATC	0.000	5.155

	Mean	Stdev
twist-11-GATC	38.883	4.453

	Mean	Stdev
rise-12-ATAT	3.331	0.260

	Mean	Stdev
roll-12-ATAT	-2.229	4.657

	Mean	Stdev
shift-12-ATAT	0.000	0.506

	Mean	Stdev
slide-12-ATAT	-0.850	0.365

	Mean	Stdev
tilt-12-ATAT	0.000	3.569

	Mean	Stdev
twist-12-ATAT	32.010	3.568

	Mean	Stdev
rise-13-TCGA	3.408	0.289

	Mean	Stdev
roll-13-TCGA	0.972	5.482

	Mean	Stdev
shift-13-TCGA	0.000	1.067

	Mean	Stdev
slide-13-TCGA	0.088	0.566

	Mean	Stdev
tilt-13-TCGA	0.000	5.347

	Mean	Stdev
twist-13-TCGA	38.437	4.738

	Mean	Stdev
rise-14-CGCG	3.051	0.330

	Mean	Stdev
roll-14-CGCG	7.986	5.881

	Mean	Stdev
shift-14-CGCG	0.000	0.909

	Mean	Stdev
slide-14-CGCG	0.040	0.531

	Mean	Stdev
tilt-14-CGCG	0.000	5.409

	Mean	Stdev
twist-14-CGCG	28.942	7.553

	Mean	Stdev
rise-15-GATC	3.372	0.283

	Mean	Stdev
roll-15-GATC	0.974	5.570

	Mean	Stdev
shift-15-GATC	0.000	0.941

	Mean	Stdev
slide-15-GATC	0.026	0.588

	Mean	Stdev
tilt-15-GATC	0.000	4.697

	Mean	Stdev
twist-15-GATC	36.989	5.523

	Mean	Stdev
rise-16-AGCT	3.416	0.314

	Mean	Stdev
roll-16-AGCT	1.771	5.077

	Mean	Stdev
shift-16-AGCT	0.000	0.760

	Mean	Stdev
slide-16-AGCT	-0.680	0.581

	Mean	Stdev
tilt-16-AGCT	0.000	4.704

	Mean	Stdev
twist-16-AGCT	34.341	5.012

	Mean	Stdev
rise-2-CGCG	3.241	0.314

	Mean	Stdev
roll-2-CGCG	6.598	6.097

	Mean	Stdev
shift-2-CGCG	0.000	1.099

	Mean	Stdev
slide-2-CGCG	0.152	0.509

	Mean	Stdev
tilt-2-CGCG	0.000	6.341

	Mean	Stdev
twist-2-CGCG	34.280	6.209

	Mean	Stdev
rise-3-GATC	3.408	0.291

	Mean	Stdev
roll-3-GATC	1.506	5.271

	Mean	Stdev
shift-3-GATC	0.000	1.079

	Mean	Stdev
slide-3-GATC	0.103	0.587

	Mean	Stdev
tilt-3-GATC	0.000	5.386

	Mean	Stdev
twist-3-GATC	38.387	4.468

	Mean	Stdev
rise-4-ATAT	3.301	0.260

	Mean	Stdev
roll-4-ATAT	-2.220	4.679

	Mean	Stdev
shift-4-ATAT	0.000	0.526

	Mean	Stdev
slide-4-ATAT	-0.852	0.360

	Mean	Stdev
tilt-4-ATAT	0.000	3.647

	Mean	Stdev
twist-4-ATAT	31.985	3.521

	Mean	Stdev
rise-5-TCGA	3.405	0.292

	Mean	Stdev
roll-5-TCGA	1.184	5.554

	Mean	Stdev
shift-5-TCGA	0.000	0.989

	Mean	Stdev
slide-5-TCGA	0.009	0.604

	Mean	Stdev
tilt-5-TCGA	0.000	5.211

	Mean	Stdev
twist-5-TCGA	38.419	5.003

	Mean	Stdev
rise-6-CGCG	3.105	0.342

	Mean	Stdev
roll-6-CGCG	8.691	6.029

	Mean	Stdev
shift-6-CGCG	0.000	0.907

	Mean	Stdev
slide-6-CGCG	0.013	0.509

	Mean	Stdev
tilt-6-CGCG	0.000	5.343

	Mean	Stdev
twist-6-CGCG	28.977	7.482

	Mean	Stdev
rise-7-GATC	3.436	0.290

	Mean	Stdev
roll-7-GATC	0.943	5.644

	Mean	Stdev
shift-7-GATC	0.000	0.947

	Mean	Stdev
slide-7-GATC	-0.035	0.599

	Mean	Stdev
tilt-7-GATC	0.000	4.887

	Mean	Stdev
twist-7-GATC	38.997	4.576

	Mean	Stdev
rise-8-ATAT	3.303	0.261

	Mean	Stdev
roll-8-ATAT	-1.784	4.668

	Mean	Stdev
shift-8-ATAT	0.000	0.549

	Mean	Stdev
slide-8-ATAT	-0.841	0.362

	Mean	Stdev
tilt-8-ATAT	0.000	3.547

	Mean	Stdev
twist-8-ATAT	31.462	3.808

	Mean	Stdev
rise-9-TCGA	3.448	0.297

	Mean	Stdev
roll-9-TCGA	0.978	5.543

	Mean	Stdev
shift-9-TCGA	0.000	0.940

	Mean	Stdev
slide-9-TCGA	-0.022	0.601

	Mean	Stdev
tilt-9-TCGA	0.000	4.911

	Mean	Stdev
twist-9-TCGA	39.142	4.440

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