NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	6.449	0.000	0.000	0.016	0.000	0.000
Slide	0.000	36.146	-1.827	0.000	-0.235	-0.163
Rise	0.000	-1.827	53.607	0.000	0.047	0.272
Tilt	0.017	0.000	0.000	0.000	0.000	0.000
Roll	0.000	-0.235	0.047	0.000	0.055	-0.000
Twist	0.000	-0.163	0.272	0.000	-0.000	0.015

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	5.225	0.000	0.000	0.013	0.000	0.000
Slide	0.000	39.026	0.082	0.000	-0.188	0.269
Rise	0.000	0.080	55.374	0.000	-0.030	0.232
Tilt	0.013	0.000	0.000	0.000	0.000	0.000
Roll	0.000	-0.188	-0.030	0.000	0.056	0.002
Twist	0.000	0.269	0.233	0.000	0.002	0.013

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	17.239	0.000	0.000	0.039	0.000	0.000
Slide	0.000	15.007	-0.005	0.000	-0.011	0.000
Rise	0.000	0.046	0.381	0.000	0.000	0.094
Tilt	0.039	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.008	0.000	0.000	0.000	0.000
Twist	0.000	0.011	0.094	0.000	-0.000	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	12.257	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	17.989	0.752	0.000	-0.016	0.216
Rise	0.000	1.029	0.413	0.000	-0.002	0.093
Tilt	-0.053	0.000	0.000	0.018	0.000	0.000
Roll	0.000	-0.018	-0.002	0.000	0.000	-0.000
Twist	0.000	0.266	0.092	0.000	-0.000	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.396	0.000	0.000	0.039	0.000	0.000
Slide	0.000	6.865	2.770	0.000	-0.054	-0.000
Rise	0.000	2.770	1.703	0.000	0.020	-0.031
Tilt	0.039	0.000	0.000	0.003	0.000	0.000
Roll	0.000	-0.054	0.020	0.000	0.028	-0.007
Twist	0.000	-0.000	-0.031	0.000	-0.007	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.407	0.000	0.000	0.055	0.000	0.000
Slide	0.000	5.860	2.410	0.000	-0.066	-0.012
Rise	0.000	2.410	5.669	0.000	0.082	-0.105
Tilt	0.055	0.000	0.000	0.003	0.000	0.000
Roll	0.000	-0.066	0.082	0.000	0.032	-0.005
Twist	0.000	-0.012	-0.105	0.000	-0.005	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.472	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	2.926	-0.960	0.000	-0.021	-0.091
Rise	0.000	-0.960	1.213	0.000	0.021	-0.047
Tilt	-0.043	0.000	0.000	0.005	0.000	0.000
Roll	0.000	-0.021	0.021	0.000	0.028	0.004
Twist	0.000	-0.091	-0.047	0.000	0.004	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.490	0.000	0.000	0.047	0.000	0.000
Slide	0.000	1.873	-0.234	0.000	0.022	0.002
Rise	0.000	-0.234	1.226	0.000	0.103	-0.069
Tilt	0.047	0.000	0.000	0.003	0.000	0.000
Roll	0.000	0.022	0.103	0.000	0.016	-0.003
Twist	0.000	0.002	-0.069	0.000	-0.003	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.129	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	3.179	0.847	0.000	-0.032	-0.090
Rise	0.000	0.847	0.831	0.000	0.062	-0.073
Tilt	-0.011	0.000	0.000	0.002	0.000	0.000
Roll	0.000	-0.032	0.062	0.000	0.022	-0.000
Twist	0.000	-0.090	-0.073	0.000	-0.000	0.017

[Image courtesy of Curves+]

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	Mean	Stdev
-gggg.10.cte	0.000	0.000

	Mean	Stdev
-gggg.2.cte	0.000	0.000

	Mean	Stdev
-gggg.3.cte	0.000	0.000

	Mean	Stdev
-gggg.9.cte	0.000	0.000

	Mean	Stdev
-gttg.4.cte	0.000	0.000

	Mean	Stdev
-tggt.8.cte	0.000	0.000

	Mean	Stdev
-tttt.5.cte	0.000	0.000

	Mean	Stdev
-tttt.6.cte	0.000	0.000

	Mean	Stdev
-tttt.7.cte	0.000	0.000

	Mean	Stdev
rise-10-GGGG	-0.005	0.110

	Mean	Stdev
roll-10-GGGG	0.618	5.253

	Mean	Stdev
shift-10-GGGG	0.000	0.548

	Mean	Stdev
slide-10-GGGG	-0.000	0.133

	Mean	Stdev
tilt-10-GGGG	86.855	86.365

	Mean	Stdev
twist-10-GGGG	0.334	4.713

	Mean	Stdev
rise-2-GGGG	0.023	0.108

	Mean	Stdev
roll-2-GGGG	-0.478	5.982

	Mean	Stdev
shift-2-GGGG	0.000	0.555

	Mean	Stdev
slide-2-GGGG	-0.012	0.135

	Mean	Stdev
tilt-2-GGGG	86.911	85.967

	Mean	Stdev
twist-2-GGGG	-0.989	5.393

	Mean	Stdev
rise-3-GGGG	0.875	2.589

	Mean	Stdev
roll-3-GGGG	79.929	90.357

	Mean	Stdev
shift-3-GGGG	0.000	0.185

	Mean	Stdev
slide-3-GGGG	-0.268	0.229

	Mean	Stdev
tilt-3-GGGG	0.000	4.318

	Mean	Stdev
twist-3-GGGG	-0.442	6.281

	Mean	Stdev
rise-4-GTTG	2.679	1.089

	Mean	Stdev
roll-4-GTTG	15.331	4.999

	Mean	Stdev
shift-4-GTTG	0.000	0.849

	Mean	Stdev
slide-4-GTTG	-0.042	0.537

	Mean	Stdev
tilt-4-GTTG	0.000	19.404

	Mean	Stdev
twist-4-GTTG	-24.533	6.342

	Mean	Stdev
rise-5-TTTT	4.534	1.043

	Mean	Stdev
roll-5-TTTT	-16.847	4.796

	Mean	Stdev
shift-5-TTTT	0.000	2.411

	Mean	Stdev
slide-5-TTTT	-0.221	0.678

	Mean	Stdev
tilt-5-TTTT	0.000	23.556

	Mean	Stdev
twist-5-TTTT	26.373	7.382

	Mean	Stdev
rise-6-TTTT	4.535	1.230

	Mean	Stdev
roll-6-TTTT	30.447	10.043

	Mean	Stdev
shift-6-TTTT	0.000	0.574

	Mean	Stdev
slide-6-TTTT	3.974	0.615

	Mean	Stdev
tilt-6-TTTT	0.000	16.344

	Mean	Stdev
twist-6-TTTT	-68.634	5.500

	Mean	Stdev
rise-7-TTTT	6.127	1.579

	Mean	Stdev
roll-7-TTTT	-21.486	7.199

	Mean	Stdev
shift-7-TTTT	0.000	2.771

	Mean	Stdev
slide-7-TTTT	-0.043	0.574

	Mean	Stdev
tilt-7-TTTT	0.000	20.829

	Mean	Stdev
twist-7-TTTT	37.592	7.940

	Mean	Stdev
rise-8-TGGT	3.469	0.383

	Mean	Stdev
roll-8-TGGT	14.890	4.539

	Mean	Stdev
shift-8-TGGT	0.000	1.099

	Mean	Stdev
slide-8-TGGT	-0.305	0.362

	Mean	Stdev
tilt-8-TGGT	0.000	22.575

	Mean	Stdev
twist-8-TGGT	-18.342	5.217

	Mean	Stdev
rise-9-GGGG	1.212	2.558

	Mean	Stdev
roll-9-GGGG	117.562	82.669

	Mean	Stdev
shift-9-GGGG	0.000	0.220

	Mean	Stdev
slide-9-GGGG	0.066	0.242

	Mean	Stdev
tilt-9-GGGG	0.074	5.589

	Mean	Stdev
twist-9-GGGG	-4.002	6.761

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3'	G	-	G	-	G	-	G	-	T	-	T	-	T	-	T	-	G	-	G	-	G	-	G	5'
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