Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines.
| Title | Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines. |
| Publication Type | Journal Article |
| Year of Publication | 2002 |
| Authors | Aviñó, Anna, Frieden Miriam, Morales Juan Carlos, de la Torre Beatriz García, García Ramón Güimil, Azorín Ferran, Gelpí Josep-Lluis, Orozco Modesto, González Carlos, and Eritja Ramon |
| Journal | Nucleic Acids Res |
| Volume | 30 |
| Pagination | 2609-19 |
| Date Published | 2002 Jun 15 |
| ISSN | 1362-4962 |
| Keywords | Amines, Base Sequence, Biomolecular, Circular Dichroism, DNA, Electrophoretic Mobility Shift Assay, Guanine, Hot Temperature, Models, Molecular, Nuclear Magnetic Resonance, Nucleic Acid Conformation, Nucleic Acid Denaturation, Oligonucleotides, Purines, Pyrimidines, Salts |
| Abstract | Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence. |
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