Prevalent Sequences in the Human Genome Can Form Mini i-Motif Structures at Physiological pH
Title | Prevalent Sequences in the Human Genome Can Form Mini i-Motif Structures at Physiological pH |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Mir, Bartomeu, Serrano Israel, Buitrago Diana, Orozco Modesto, Escaja Núria, and González Carlos |
Journal | Journal of the American Chemical Society |
Volume | 139 |
Issue | 40 |
Pagination | 13985 - 13988 |
Date Published | 11/2017 |
ISBN Number | 0002-7863 |
Abstract | We report here the solution structure of several repetitive DNA sequences containing d(TCGTTCCGT) and related repeats. At physiological pH, these sequences fold into i-motif like quadruplexes in which every two repeats a globular structure is stabilized by two hemiprotonated C:C+ base pairs, flanked by two minor groove tetrads resulting from the association of G:C or G:T base pairs. The interaction between the minor groove tetrads and the nearby C:C+ base pairs affords a strong stabilization, which results in effective pHT values above 7.5. Longer sequences with more than two repeats are able to fold in tandem, forming a rosary bead-like structure. Bioinformatics analysis shows that these sequences are prevalent in the human genome, and are present in development-related genes.We report here the solution structure of several repetitive DNA sequences containing d(TCGTTCCGT) and related repeats. At physiological pH, these sequences fold into i-motif like quadruplexes in which every two repeats a globular structure is stabilized by two hemiprotonated C:C+ base pairs, flanked by two minor groove tetrads resulting from the association of G:C or G:T base pairs. The interaction between the minor groove tetrads and the nearby C:C+ base pairs affords a strong stabilization, which results in effective pHT values above 7.5. Longer sequences with more than two repeats are able to fold in tandem, forming a rosary bead-like structure. Bioinformatics analysis shows that these sequences are prevalent in the human genome, and are present in development-related genes. |
URL | https://dx.doi.org/10.1021/jacs.7b07383 |
Short Title | J. Am. Chem. Soc. |