Allosterism and signal transfer in DNA

TitleAllosterism and signal transfer in DNA
Publication TypeJournal Article
Year of Publication2018
AuthorsBalaceanu, Alexandra, Pérez Alberto, Dans Pablo D., and Orozco Modesto
JournalNucleic Acids Research
Start Page7554
Pagination7554 - 7565
Date Published2018/09/06
ISBN Number0305-1048

We analysed the basic mechanisms of signal transmission in DNA and the origins of the allostery exhibited by systems such as the ternary complex BAMHI–DNA–GRDBD. We found that perturbation information generated by a primary protein binding event travels as a wave to distant regions of DNA following a hopping mechanism. However, such a structural perturbation is transient and does not lead to permanent changes in the DNA geometry and interaction properties at the secondary binding site. The BAMHI–DNA–GRDBD allosteric mechanism does not occur through any traditional models: direct (protein-protein), indirect (reorganization of the secondary site) readout or solvent-release. On the contrary, it is generated by a subtle and less common entropy-mediated mechanism, which might have an important role to explain other DNA-mediated cooperative effects.

Short TitleNucleic Acids Research